Solubility of Dimagnesium Phosphate Trihydrate and Trimagnesium Phosphate

Racz, G. J.; Soper, R. J.

doi:10.4141/cjss68-036

Cited by 20 publications

(16 citation statements)

References 8 publications

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“…The equilibrium constant for the formation of soluble MgHPO 4 aggregates (K eq,MgHPO4 ) showed an average value of 1.31 ± 0.06 mM, closely matching the 0.97 ± 0.05 mM value reported in the literature 24 (Fig. 3a).…”

Section: Validation Of Systems Nmr Derived Reaction Constantssupporting

confidence: 87%

“…k ex = linewidth ⋅ π (23) k ex = k ex, un f olding + k ex, base − f lipping + R 2 (24) The contribution of B0 field inhomogeneity (R 2(B0) ) is considered negligible. The combined contribution of base-flipping (k ex,base-flipping ) 31 and transverse relaxation rate (R 2 ) was determined from linewidths of imino signals in purified, SMN2 hairpin additionally stabilized by terminal GCs (13-bp, GGCGGGUUUUGGC-AGAC-GCCAAAAUCCGCC).…”

Section: Rna Folding δG From Imino Signal Lineshape Analysismentioning

confidence: 99%

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Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis

et al. 2019

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Cellular behavior is controlled by the interplay of diverse biomolecules. Most experimental methods, however, can monitor only a single molecule class or reaction type at a time. We developed an in vitro Nuclear Magnetic Resonance spectroscopy (NMR) approach, which permitted dynamic quantification of an entire "heterotypic" network -simultaneously monitoring three distinct molecule classes (metabolites, proteins, RNA) and all elementary reaction types (bimolecular interactions, catalysis, unimolecular changes). Focusing on an 8-reaction cotranscriptional RNA folding network, in a single sample we recorded over 35 time-points with over 170 observables each, and accurately determined 5 core reaction constants in multiplex. This reconstruction revealed unexpected cross-talk between the different reactions. We further observed dynamic phase-separation in a system of five distinct RNA binding domains in the course of the RNA transcription reaction. Our Systems NMR approach provides a deeper understanding of biological network dynamics by combining the dynamic resolution of biochemical assays and the multiplexing ability of "omics".Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:

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Section: Validation Of Systems Nmr Derived Reaction Constantssupporting

confidence: 87%

Section: Rna Folding δG From Imino Signal Lineshape Analysismentioning

confidence: 99%

Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis

et al. 2019

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“…Solutions buffered with HEPES and Tris‐HCl were evaluated for the separation of the substrates and products. Although phosphate buffer worked for the bacterial holo‐ACC assay , the interaction of phosphate and Mg 2+ could negatively impact assay reproducibility due to the low solubility products of Mg 2+ with phosphate in aqueous solution . HEPES (pK a 7.55) and Tris‐HCl (pK a 8.3) were tested as alternatives to phosphate for the ACC enzyme assay based on previous use .…”

Section: Resultsmentioning

confidence: 99%

Capillary electrophoretic assay of human acetyl‐coenzyme A carboxylase 2

2019

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Human acetyl‐coenzyme A carboxylase 2 catalyzes the carboxylation of acetyl coenzyme A to form malonyl coenzyme A, along with the conversion of magnesium‐adenosine triphosphate complex to magnesium‐adenosine diphosphate complex. A simple off‐column capillary electrophoresis assay for human acetyl‐coenzyme A carboxylase 2 was developed based on the separation of magnesium‐adenosine triphosphate complex, magnesium‐adenosine diphosphate complex, acetyl coenzyme A and malonyl coenzyme A with detection by ultraviolet absorption at 256 nm. When Mg2+ was absent from the separation buffer, the zones due to magnesium‐adenosine triphosphate complex and magnesium‐adenosine diphosphate complex both split and migrated as two separate peaks. With Mg2+ added to the separation buffer, magnesium‐adenosine triphosphate complex and magnesium‐adenosine diphosphate complex produced single peaks, and the reproducibility of peak shape and area improved for human acetyl‐coenzyme A carboxylase 2 assay components. The final separation buffer used was 30.0 mM HEPES, 3.0 mM MgCl2, 2.5 mM KHCO3, and 2.5 mM potassium citrate at pH 7.50. The same buffer was used for the enzyme‐catalyzed reaction (off‐column). Inhibition of human acetyl‐coenzyme A carboxylase 2 by CP‐640186, a known inhibitor, was detected using the capillary electrophoresis assay.

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“…Results from XRD show that P exists in the form of hydrated Mg 3 (PO 4 ) 2 and MgHPO 4 (Figure 4). Since MgHPO 4 .3H 2 0 is more soluble (pK SP = 5.83) than Mg 3 (PO 4 ) 2 •22H 2 O (pK SP = 23.7) and Mg 3 (PO 4 ) 2 (pK SP = 23.28) (Recz & Soron, 1968), it is therefore likely that enrichment of B with potassium phosphate solution enhanced the content of more soluble MgHPO 4 in B50, B100 and B150. The increased solubility of MgHPO 4 in B50, B100 and B150 is probably responsible for their better P availability compared with B.…”

Section: Magnesium (Mg) and Phosphorus (P) Concentrations Of Biocharmentioning

confidence: 99%

Phosphorus availability from magnesium‐modified P‐enriched Douglas fir biochar as a controlled release fertilizer

Arwenyo

Varco

Dygert

et al. 2021

Soil Use and Management

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Phosphorus (P) is one of the essential elements required for plant growth and development. However, worldwide, many agricultural soils can be deficient in P.The use of fertilizers and manures as a source of P can be costly, limited in supply in some regions of the world and ecologically unfavourable. Nutrient-enriched biochar has been suggested as a relatively cost-effective and eco-friendly P source.This study investigated P availability from Douglas fir biochar modified with magnesium chloride and potassium hydroxide solutions only (B), and modified P-

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Solubility of Dimagnesium Phosphate Trihydrate and Trimagnesium Phosphate

Abstract: The solubilities in water "t .tlJJfif1ho,ohr." trihydrate O4cFPo: 3 HrO ). trimagnesium phosphatc trvenry-two hydratc (.\lgr ( PO, )r' 22HrO ). and anhydrous triinagnesium phosphatc (lig"(PO,)=l were diicrmined. The solubiliiics of these piosphates (pii.sp1*..e"?ounl'to be 5.81i0.01, 21

Cited by 20 publications

References 8 publications

Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis

Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis

Capillary electrophoretic assay of human acetyl‐coenzyme A carboxylase 2

Phosphorus availability from magnesium‐modified P‐enriched Douglas fir biochar as a controlled release fertilizer

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Solubility of Dimagnesium Phosphate Trihydrate and Trimagnesium Phosphate

Abstract: The solubilities in water "t .t*lJJfif1ho,ohr." trihydrate O4cFPo*: 3 HrO ). trimagnesium phosphatc trvenry-two hydratc (.\lgr ( PO, )r' 22HrO ). and anhydrous triinagnesium phosphatc (lig"(PO,)=l were diicrmined. The solubiliiics of these piosphates (pii.sp1*..e"?ounl'to be 5.81i0.01, 21

Cited by 20 publications

References 8 publications

Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis

Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis

Capillary electrophoretic assay of human acetyl‐coenzyme A carboxylase 2

Phosphorus availability from magnesium‐modified P‐enriched Douglas fir biochar as a controlled release fertilizer

Contact Info

Product

Resources

About

Abstract: The solubilities in water "t .tlJJfif1ho,ohr." trihydrate O4cFPo: 3 HrO ). trimagnesium phosphatc trvenry-two hydratc (.\lgr ( PO, )r' 22HrO ). and anhydrous triinagnesium phosphatc (lig"(PO,)=l were diicrmined. The solubiliiics of these piosphates (pii.sp1*..e"?ounl'to be 5.81i0.01, 21