Solubility Measurements of Surfactants and Dissolution of Cytochrome c in 1,1,1,2‐Tetrafluoroethane Modified with Organic Solvents

Abstract

The solubilities of four surfactants, sodium bis-2-ethylhexyl sulfosuccinate (AOT), cetyltrimethylammonium chloride, didodecyldimethylammonium bromide, and n-dodecyltrimethylammonium chloride in liquid refrigerant 1,1,1,2-tetrafluoroethane (R134a) modified with methanol, ethanol, and 2-propanol were measured. The measurements were performed under 25 and 50 bar at 25°C. The solubility of AOT was as high as 92 mM in R134a with addition of 2% ethanol. Cytochrome c molecules were then verified to be soluble in the AOT-containing and ethanol-modified R134a solution. Accordingly, liquid R134a is suggested as a potential extraction solvent for hydrophilic compounds.