Solubility enhancement andin vitroevaluation of PEG-b-PLA micelles as nanocarrier of semi-synthetic andrographolide analogue for cholangiocarcinoma chemotherapy

Abstract

Nanoencapsulation of AG 050-P implicated its potential development for clinical use in CCA treatment.

Enhancement of the aqueous solubility and permeability of a poorly water soluble drug ritonavir via lyophilized milk-based solid dispersions

“…These approaches include, and are not limited to salt formation, solid-dispersions, prodrug formation, complexation, particle size modifications, micellization, micro-and nano-technological approaches such as micro-emulsions, micro-suspensions, nanoemulsions, nano-suspensions, solid-lipid nanoparticles, etc. [10][11][12][13][14][15][16][17][18][19][20][21][22] The term solid dispersions (SD) has been described earlier as a dispersion of one or more Active Pharmaceutical Ingredient (API) in an inert carrier or a matrix in solid state, prepared by solvent, melting or solvent-melting method. 23 The API in a SD can be dispersed as separate molecules, amorphous particles or crystalline particles; while the carrier can be in a crystalline or amorphous state.…”

Preparation and Characterizations of RSPP050-Loaded Polymeric Micelles Using Poly(ethylene glycol)-<i>b</i>-Poly(ε-caprolactone) and Poly(ethylene glycol)-<i>b</i>-Poly(D,L-lactide)

“…DMSO (5 mL) was added to the lyophilized sample, and the AG50 content was measured at the wavelength of 260 nm. 26) Moreover, AG50 solubility in PBS containing 10 units of porcine esterase (PLE/PBS) was determined by incubating excess AG50 (1 mg) in 1 mL (n=3) of each esterase solution at 37°C for 24 h with agitation. Samples were centrifuged at 10000 rpm for 20 min to separate a supernatant.…”

“…At desired time intervals, 20 mL release media was taken out and replenished with an equal volume of fresh media and added 2 mL of ethanol to measure the amount of AG50 by UV-Vis spectrophotometer. 26) This experiment was carried out in triplicate and the release curve was fitted with different mathematical models including Zero order, First order, Higuchi, Korsmeyer-Peppas, HixsonCrowell, Hopfenberg, Baker-Lonsale, Makoid-Banakar, Peppas-Sahlin, Quadratic, Weibull and Logistic Model. 33) Cytotoxicity Study of AG50-Loaded Copolymeric Micelles Cytotoxicity test of micelles was carried out against cholangiocarcinoma cells (HuCCT1) and was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay following the standard procedure (Molecular Probes, V-13154).…”

Glucose-installed, SPIO-loaded PEG-b-PCL micelles as MR contrast agents to target prostate cancer cells

“…Polymeric micelles can be prepared from biocompatible amphiphilic block copolymers, forming core-shell architecture. The hydrophobic core acts as a reservoir for water-insoluble compounds resulting in the significant increase in solubility and stability of these compounds (Puntawee et al 2016). The hydrophilic shell protects polymeric micelles from mononuclear phagocytic system (MPS) (Jokerst et al 2011), and reduces renal clearance (Movassaghian et al 2015).…”