Solubility and transport phenomena in perfluorochemicals relevant to blood substitution and other biomedical applications

Riess, Jean G.; Blanc, Maurice

doi:10.1351/pac198254122383

Cited by 278 publications

(122 citation statements)

References 0 publications

Supporting

Mentioning

119

Contrasting

Unclassified

Order By: Relevance

“…As mentioned, numerous attempts have been made to use perfluorocarbons as an artificial blood substitute or to improve tissue oxygenation (20)(21)(22). Perfluorocarbons are also suggested to find a role in PDT by maintaining tissue oxygenation and thereby enhancing the PDT efficacy after light activation of the photosensitizer (33).…”

Section: Discussionmentioning

confidence: 99%

“…They dissolve approximately 20-fold more oxygen than water or plasma. Since the demonstration of perfluorocarbons as oxygen carrying fluids for mice (19), numerous attempts have been made to use perfluorocarbons as an artificial blood substitute or to improve tissue oxygenation (20)(21)(22). Many perfluorocarbons are relatively non-toxic and, due to their high oxygen-carrying capacity, it is believed that these compounds can be used as oxygen carriers to enhance the PDT efficacy of photosensitizers.…”

Section: Enhancing the Photodynamic Effect Of Hypericin In Human Bladmentioning

confidence: 99%

See 1 more Smart Citation

Enhancing the photodynamic effect of hypericin in human bladder transitional cell carcinoma spheroids by the use of the oxygen carrier, perfluorodecalin

Kamuhabwa¹,

Huygens²,

Roskams³

et al. 2006

Int J Oncol

View full text Add to dashboard Cite

Abstract. In the present study, we evaluated the possibility of enhancing the photodynamic effect of hypericin in transitional cell carcinoma (TCC) spheroids by the use of the oxygen carrier, perfluorodecalin. Following incubation with hypericin, RT-112 TCC spheroids were irradiated in the presence or absence of perfluorodecalin, at light doses of 7 J/cm 2 or 28 J/ cm 2 , delivered at a fluence rate of 15 mW/cm 2 . The photodynamic therapy (PDT) efficacy was evaluated and apoptotic cells were visualized. The results show that, in the absence of perfluorodecalin, spheroidal TCC cells are inadequately sensitive to hypericin PDT. As was shown by fluorescence microscopy, this lack of activity was not due to insufficient photoactive concentrations of hypericin reaching the inner parts of the spheroids. Conversely, enhanced oxygenation of spheroids by perfluorodecalin led to a dramatic enhancement of hypericin PDT efficacy. The detection of nuclear shrinkage or fragmentation with DAPI staining and the assessment of cell morphology by light microscopy indicated that apoptosis was the most prominent response of spheroids to hypericin PDT in the presence of perfluorodecalin. In conclusion, the results of this study suggest that perfluorocarbons, such as perfluorodecalin, are useful in enhancing the oxygenation of tumor tissue, resulting in highly efficient hypericin PDT. Since hypericin becomes concentrated specifically in human bladder urothelial carcinoma lesions and the bladder is very well suited to instillation with a perfluorocarbon, combining the techniques looks very promising for an efficient and selective whole bladder wall photodynamic antitumoral treatment in a urological clinical setting.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Enhancing the Photodynamic Effect Of Hypericin In Human Bladmentioning

confidence: 99%

Enhancing the photodynamic effect of hypericin in human bladder transitional cell carcinoma spheroids by the use of the oxygen carrier, perfluorodecalin

Kamuhabwa¹,

Huygens²,

Roskams³

et al. 2006

Int J Oncol

View full text Add to dashboard Cite

show abstract

“…5A and B), which is in agreement with previously reported results 50,64-66 . For continuous cell microencapsulation, the high oxygen permeability of PDMS microfluidic devices 67 , combined with the high oxygen solubility of the fluorocarbon oil used in these studies 68 necessitates the use of a custom microfluidic design. 38 This device, a nitrogen-jacketed double layer device (Fig.…”

Section: Resultsmentioning

confidence: 99%

A microfluidic-based cell encapsulation platform to achieve high long-term cell viability in photopolymerized PEGNB hydrogel microspheres

et al. 2017

View full text Add to dashboard Cite

Cell encapsulation within photopolymerized polyethylene glycol (PEG)-based hydrogel scaffolds has been demonstrated as a robust strategy for cell delivery, tissue engineering, regenerative medicine, and developing in vitro platforms to study cellular behavior and fate. Strategies to achieve spatial and temporal control over PEG hydrogel mechanical properties, chemical functionalization, and cytocompatibility have advanced considerably in recent years. Recent microfluidic technologies have enabled the miniaturization of PEG hydrogels, thus enabling the fabrication of miniaturized cell-laden vehicles. However, rapid oxygen diffusive transport times on the microscale dramatically inhibit chain growth photopolymerization of polyethylene glycol diacrylate (PEGDA), thus decreasing the viability of cells encapsulated within these microstructures. Another promising PEG-based scaffold material, PEG norbornene (PEGNB), is formed by a step-growth photopolymerization and is not inhibited by oxygen. PEGNB has also been shown to be more cytocompatible than PEGDA and allows for orthogonal addition reactions. The step-growth kinetics, however, are slow and therefore challenging to fully polymerize within droplets flowing through microfluidic devices. Here, we describe a microfluidic-based droplet fabrication platform that generates consistently monodisperse cell-laden water-in-oil emulsions. Microfluidically generated PEGNB droplets are collected and photopolymerized under UV exposure in bulk emulsions. In this work, we compare this microfluidic-based cell encapsulation platform with a vortex-based method on the basis of microgel size, uniformity, post-encapsulation cell viability and long-term cell viability. Several factors that influence post-encapsulation cell viability were identified. Finally, long-term cell viability achieved by this platform was compared to a similar cell encapsulation platform using PEGDA. We show that this PEGNB microencapsulation platform is capable of generating cell-laden hydrogel microspheres at high rates with well-controlled size distributions and high long-term cell viability.

show abstract

“…The desirable characteristics in the second generation PFC, as advocated by Reiss and Le Blanc et al [16] were : 1) large oxygen-dissolving capacity, 2) faster excretion and less tissue retention, 3) lack of significant side effects, 4) increasing purity, and 5) large scale production and availability. The three candidates chosen as per these criteria were PFD, perfluorooctyl bromide (PFOB) and bis (perfluorobutyl) ethylene.…”

Section: Second Generation Perfluorocarbonsmentioning

confidence: 99%

Artifical Blood

Goorha¹,

Deb²,

Chatterjee

et al. 2003

Medical Journal Armed Forces India

View full text Add to dashboard Cite

Elimination of unwanted side-effects, especially transfusion-transmitted diseases (HIV and hepatitis) and leucocyte-mediated allosensitisation, is an important goal of modern transfusion medicine. The problems and high cost factor involved in collecting and storing human blood and the pending world-wide shortages are the other driving forces contributing towards the development of blood substitutes. Two major areas of research in this endeavour are haemoglobin-based oxygen carriers (HBOCs) and perfluorochemicals. Even though they do not qualify as perfect red blood cell substitutes, these 'oxygen carrying solutions' have many potential clinical and non clinical usages. These can reach tissues more easily than normal red cells and can deliver oxygen directly. These are not without adverse effects, and extensive clinical trials are being conducted to test their safety and efficacy. New understandings on the mode of action of these products will help to define their utility and application. Only after successful clinical trials can they be used for patient management, after approval by the FDA. MJAFI 2003; 59 : 45-50Key Words : Blood substitute; Haemoglobin solutions; Perfluorocarbons main advantages include availability in large volumes, storage for prolonged periods, rapid administration (without typing and cross matching) and sterilisation by pasteurisation. Their main known disadvantages are, reduced circulation half-life, haemodynamic and gastrointestinal perturbations, probably related to nitric oxide (NO) scavenging, free radical induction, and alterations of biochemical and haematological parameters (increase in liver enzyme levels, platelet aggregation) [1][2][3]. Purified haemoglobin solutionsThe first step in the pursuit for red cell substitute was

show abstract

Solubility and transport phenomena in perfluorochemicals relevant to blood substitution and other biomedical applications

Abstract: Abstract

Cited by 278 publications

References 0 publications

Enhancing the photodynamic effect of hypericin in human bladder transitional cell carcinoma spheroids by the use of the oxygen carrier, perfluorodecalin

Enhancing the photodynamic effect of hypericin in human bladder transitional cell carcinoma spheroids by the use of the oxygen carrier, perfluorodecalin

A microfluidic-based cell encapsulation platform to achieve high long-term cell viability in photopolymerized PEGNB hydrogel microspheres

Artifical Blood

Contact Info

Product

Resources

About