Solubility and subcellular localization of the three <i>Drosophila </i><scp>RDGC</scp> phosphatase variants are determined by acylation

Strauch, Lisa; Pfannstiel, Jens; Huber, Armin; Voolstra, Olaf

doi:10.1002/1873-3468.13163

Cited by 3 publications

(7 citation statements)

References 31 publications

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“…2M) and supports the validity of the screening. RdgC is a Ser-Thr protein phosphatase, which mediates rhodopsin phosphorylation (Strauch et al, 2018). The loss of RdgC causes retinal degeneration (Vinós et al, 1997;Kiselev et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Activity-dependent endocytosis of Wingless regulates synaptic plasticity in the Drosophila visual system

2020

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Section: Discussionmentioning

confidence: 99%

Activity-dependent endocytosis of Wingless regulates synaptic plasticity in the Drosophila visual system

2020

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“…Supernatants were subjected to SDS-PAGE using 8 or 12% polyacrylamide gels. Blotting, blocking, incubation with antibodies and signal detection was done as described previously [ 13 ]. Quantification of Western blot signals was done with Image Lab 5.2 (Bio-Rad) by integration of the pixel intensities of each band.…”

Section: Methodsmentioning

confidence: 99%

“…The polyclonal α-RH1, α-RDGC, and α-pS936-TRP antibodies were described before [ 9 , 13 , 20 ]. Monoclonal α-RH1 (4C5), α-TRP (Mab83F6), and α-beta-Tubulin (E7), antibodies were obtained from the Developmental Studies Hybridoma Bank.…”

Section: Methodsmentioning

confidence: 99%

“…Monoclonal α-RH1 (4C5), α-TRP (Mab83F6), and α-beta-Tubulin (E7), antibodies were obtained from the Developmental Studies Hybridoma Bank. Secondary antibodies for detection of the ECL signal were as described [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…The Drosophila rdgC gene encodes three protein isoforms, RDGC-S, -M, and -L, that harbor different N-termini. We recently reported that the RDGC-M and RDGC-L protein isoforms are tethered to the plasma membrane due to fatty acylationwhile RDGC-S does not become fatty acylated and is soluble [ 13 ]. In this work, we investigated physiological consequences of the different solubilities and subcellular localizations of the RDGC protein isoforms.…”

Section: Introductionmentioning

confidence: 99%

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Functional characterization of the three Drosophila retinal degeneration C (RDGC) protein phosphatase isoforms

et al. 2018

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Drosophila retinal degeneration C (RDGC) is the founding member of the PPEF family of protein phosphatases. RDGC mediates dephosphorylation of the visual pigment rhodopsin and the TRP ion channel. From the rdgC locus, three protein isoforms, termed RDGC-S, -M, and -L, with different N-termini are generated. Due to fatty acylation, RDGC-M and -L are attached to the plasma membrane while RDGC-S is soluble. To assign physiological roles to these RDGC isoforms, we constructed flies that express various combinations of RDGC protein isoforms. Expression of the RDGC-L isoform alone did not fully prevent rhodopsin hyperphosphorylation and resulted in impaired photoreceptor physiology and in decelerated TRP dephosphorylation at Ser936. However, expression of RDGC-L alone as well as RDGC-S/M was sufficient to prevent degeneration of photoreceptor cells which is a hallmark of the rdgC null mutant. Membrane-attached RDGC-M displayed higher activity of TRP dephosphorylation than the soluble isoform RDGC-S. Taken together, in vivo activities of RDGC splice variants are controlled by their N-termini.

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The Role of Reversible Phosphorylation of Drosophila Rhodopsin

Smylla

Wagner

Huber

2022

IJMS

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Vertebrate and fly rhodopsins are prototypical GPCRs that have served for a long time as model systems for understanding GPCR signaling. Although all rhodopsins seem to become phosphorylated at their C-terminal region following activation by light, the role of this phosphorylation is not uniform. Two major functions of rhodopsin phosphorylation have been described: (1) inactivation of the activated rhodopsin either directly or by facilitating binding of arrestins in order to shut down the visual signaling cascade and thus eventually enabling a high-temporal resolution of the visual system. (2) Facilitating endocytosis of activated receptors via arrestin binding that in turn recruits clathrin to the membrane for clathrin-mediated endocytosis. In vertebrate rhodopsins the shutdown of the signaling cascade may be the main function of rhodopsin phosphorylation, as phosphorylation alone already quenches transducin activation and, in addition, strongly enhances arrestin binding. In the Drosophila visual system rhodopsin phosphorylation is not needed for receptor inactivation. Its role here may rather lie in the recruitment of arrestin 1 and subsequent endocytosis of the activated receptor. In this review, we summarize investigations of fly rhodopsin phosphorylation spanning four decades and contextualize them with regard to the most recent insights from vertebrate phosphorylation barcode theory.

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Solubility and subcellular localization of the three Drosophila RDGC phosphatase variants are determined by acylation

Cited by 3 publications

References 31 publications

Activity-dependent endocytosis of Wingless regulates synaptic plasticity in the Drosophila visual system

Activity-dependent endocytosis of Wingless regulates synaptic plasticity in the Drosophila visual system

Functional characterization of the three Drosophila retinal degeneration C (RDGC) protein phosphatase isoforms

The Role of Reversible Phosphorylation of Drosophila Rhodopsin

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