Solidification Processes of Solutions

Viskanta, R.; Bianchi, Marcus; Critser, John K.; Gao, Dayong

doi:10.1006/cryo.1997.2015

Cited by 20 publications

(4 citation statements)

References 28 publications

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“…In particular, the width of the expansion region decreases leading to a thin, single peak with the disappearance of the double peak in the dilatation rate as cell concentration is increased. Two different expansions may be represented by the double peak, the formation of ice as well as water transport that occurs within the mushy zone [39] of the frozen zone during the cooling process. The reduction of the second peak may be explained by the cell-matrix interaction present between the MCF7 cells and ECM, which although limited, may play a role in confining this expansion as the cellular concentration quadruples.…”

Section: Discussionmentioning

confidence: 99%

Role of Cells in Freezing-Induced Cell-Fluid-Matrix Interactions Within Engineered Tissues

Seawright

Özçelikkale

Dutton

et al. 2013

Journal of Biomechanical Engineering

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During cryopreservation, ice forms in the extracellular space resulting in freezing-induced deformation of the tissue, which can be detrimental to the extracellular matrix (ECM) microstructure. Meanwhile, cells dehydrate through an osmotically driven process as the intracellular water is transported to the extracellular space, increasing the volume of fluid for freezing. Therefore, this study examines the effects of cellular presence on tissue deformation and investigates the significance of intracellular water transport and cell-ECM interactions in freezing-induced cell-fluid-matrix interactions. Freezing-induced deformation characteristics were examined through cell image deformetry (CID) measurements of collagenous engineered tissues embedded with different concentrations of MCF7 breast cancer cells versus microspheres as their osmotically inactive counterparts. Additionally, the development of a biophysical model relates the freezing-induced expansion of the tissue due to the cellular water transport and the extracellular freezing thermodynamics for further verification. The magnitude of the freezing-induced dilatation was found to be not affected by the cellular water transport for the cell concentrations considered; however, the deformation patterns for different cell concentrations were different suggesting that cell-matrix interactions may have an effect. It was therefore determined that intracellular water transport during freezing was insignificant at the current experimental cell concentrations; however, it may be significant at concentrations similar to native tissue. Finally, the cell-matrix interactions provided mechanical support on the ECM to minimize the expansion regions in the tissues during freezing.

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Section: Discussionmentioning

confidence: 99%

Role of Cells in Freezing-Induced Cell-Fluid-Matrix Interactions Within Engineered Tissues

Seawright

Özçelikkale

Dutton

et al. 2013

Journal of Biomechanical Engineering

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“…Here we use a rapid freezing process to investigate the bundling formation during water spreading on a nanorod array surface. Rapid freezing or solidification behavior is fundamental to crystal growth, refrigeration, frost formation, biomedical research and applications such as cryomicroscopy, cryopreservation, and cryosurgery [11][12][13][14][15][16][17]. In most cases, ultra-rapid freezing prevents water motion inside structures like tissues, soils, and foods.…”

Section: Introductionmentioning

confidence: 99%

Freezing a water droplet on an aligned Si nanorod array substrate

Zhao²

2008

Nanotechnology

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When a water droplet is dried on a vertically aligned Si nanorod array surface, the nanorods are bundled together. To understand how bundles are formed, a water droplet is frozen rapidly on a Si nanorod array surface observed under a cryo-SEM (scanning electron microscope). The nanorods in the precursor film form similar bundles as those dried in air. But the nanorods under the apparent frozen water droplet are only slightly deformed. We propose that the bundling of nanorods is caused by non-uniform water-nanorod interaction, which could happen either during the water spreading or drying process. Therefore, controlling the liquid-nanostructure interaction could minimize the bundling. In addition, the rapid freezing process does not preserve the water inside the nanochannels, and almost all the water forms ice on top of the nanorod surface, either as a planar interface or as particles, depending on the locations. The separated ice-nanorod interface will have potential applications in chemical separation and crystal growth.

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“…Our numerical results suggest that the cell response is greatly influenced by the location of the cells in the sample, therefore affecting the survival rate of the cells in the cell suspension. The influence of cell location on the cell water loss has been studied by Viskanta et al [44]. In his work, the freezing of a sodium chloride solution without cells was simulated.…”

Section: Application To the Interaction Of One Cell With An Ice Frontmentioning

confidence: 99%

The Front-Tracking ALE Method: Application to a Model of the Freezing of Cell Suspensions

Jaeger¹,

Carin²

2002

Journal of Computational Physics

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Solidification Processes of Solutions

Cited by 20 publications

References 28 publications

Role of Cells in Freezing-Induced Cell-Fluid-Matrix Interactions Within Engineered Tissues

Role of Cells in Freezing-Induced Cell-Fluid-Matrix Interactions Within Engineered Tissues

Freezing a water droplet on an aligned Si nanorod array substrate

The Front-Tracking ALE Method: Application to a Model of the Freezing of Cell Suspensions

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