Solidification of floating organic drop liquid‐phase microextraction cell fishing with gas chromatography–mass spectrometry for screening bioactive components from Amomum villosum Lour

Abstract

In this study, a novel solidification of floating organic drop liquid-phase microextraction cell fishing with gas chromatography-mass spectrometry (SFOD-LPME-CF-GC-MS) method was established and used to screen, isolate and analyze bioactive components from Amomum villosum Lour. extract. Through comparision of its effect on the models of normal cell and inflammatory cells, anti-inflammatory active components of essential oil from A. villosum Lour. were readily screened, and the components obtained are in agreement with related pharmacological articles. SFOD-LPME-CF-GC-MS was used to analyze the interaction of A. villosum Lour. extracts with normal and lipopolysaccharide-stimulated RAW264.7 macrophage cells. The effect of A. villosum Lour. essential oil extracts in the LPS-stimulated RAW264.7 model were also assessed in terms of cytotoxicity and nitric oxide production as an indication of bioactivity. Three potentially bioactive components were identified, demonstrating that SFOD-LPME-CF-GC-MS can be used successfully in the drug-screening process. This approach avoids the requirement for protein precipitation, but more importantly, generates a high concentration ratio, allowing analysis of trace components in traditional Chinese medicines. SFOD-LPME-CF-GC-MS is a simple, fast, effective and reliable method for the screening and analysis of bioactive components, and it can be extended to screen other bioactive components from TCMs.

An Integrative Volatile Terpenoid Profiling and Transcriptomics Analysis for Gene Mining and Functional Characterization of AvBPPS and AvPS Involved in the Monoterpenoid Biosynthesis in Amomum villosum

“…The desired characteristics of Fructus Amomi in medicine and cuisine are mainly attributed to its essential oil, in which three potentially bioactive components were identified: bornyl acetate, borneol, and camphor ( Xue et al, 2015 ). Its most active ingredient, bornyl acetate, has been reported to reduce 5-fluorouracil-induced intestinal mucositis ( Zhang et al, 2017 ).…”

“…In recent years, integration of metabolomics and transcriptomics has been developed to explore the biosynthesis pathway of the active ingredients, such as artemisinin, tanshinone, and salidroside in medicinal plants ( Gao et al, 2014 ; Ma et al, 2015 ; Wei et al, 2016 ; Torrens-Spence et al, 2018 ). Over the recent years, dried fruit has been used for the metabolic profiling of A. villosum ( Deng et al, 2005 ; Kang et al, 2013 ; Xue et al, 2015 ; Zhang et al, 2017 ). Little is known about the metabolic profiling of fresh tissues (e.g., roots, stems, leaves, seeds, and pericarp) of A. villosum growing in its native habitat, Yangchun.…”

Magnetic Solid Phase Extraction Applications Combined With Analytical Methods for Determination of Drugs in Different Matrices Review

“…Magnetic ethylendiamine-functionalized graphene oxide nanocomposite was used as the extraction sorbent for the extraction of cow milk, human urine, river, and water samples for the determination of two of the NSAIDs, naproxen and ibuprofen. This study is related to all the subsections of this review article with different matrices applyingultrasound-assisted magnetic dispersive solidphase microextraction (UAMDSPME) technique (62,63). In this technique the MNPs were dispersed in water by a compact hand-held ultrasonic and urgently injected into the sample solution for extraction.…”

Ultrasound‐assisted magnetic dispersive solid‐phase microextraction: A novel approach for the rapid and efficient microextraction of naproxen and ibuprofen employing experimental design with high‐performance liquid chromatography

“…It is clear that the choice of an appropriate and selective samplepreparation procedure is essential for accurate determination of these drugs [3][4][5][6][7]. SPME is a sample-preparation method that has been applied to a range of applications such as environmental, industrial, clinical, forensic, drug and food analysis [8][9][10].…”