Solid-State Radiation Chemistry of DNA: The Bases

Bernhard, William A.

doi:10.1016/b978-0-12-035409-2.50011-4

Cited by 149 publications

(85 citation statements)

References 146 publications

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“…DNA and DNA constituents, frozen in the solid state, provide excellent model systems in which to investigate direct-type processes (for reviews, see refs. [1][2][3][4][5]. Several variables influence the extent of direct-type damage experienced by DNA, including DNA hydration (6)(7)(8)(9)(10)(11)(12)(13)(14), conformation (6,8,9,11,14) and packing (10)(11)(12)15,16).…”

Section: Introductionmentioning

confidence: 99%

The Influence of Packing on Free Radical Yields in Solid-State DNA: Film Compared to Lyophilized Frozen Solution

Milano¹,

Bernhard²

1999

Radiation Research

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Radiation-induced free radical damage in solid-state DNA arises from direct-type damage mechanisms. In the present study, free radical yields in film and lyophilized Na DNA model systems are compared. The free radical yields in lyophilized samples are significantly greater than those in films. Since DNA conformation cannot account for the differences in free radical yields between different DNA preparations, it is proposed that the intermolecular spacing of DNA is a critical variable. The differences in the hydration dependence of free radical yields between the film and lyophilized DNA model systems are consistent with this thesis. The relatively large free radical yields observed in lyophilized DNA emphasize the fact that DNA is an extremely effective electron and hole scavenger, more so than previously thought.

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Section: Introductionmentioning

confidence: 99%

The Influence of Packing on Free Radical Yields in Solid-State DNA: Film Compared to Lyophilized Frozen Solution

Milano¹,

Bernhard²

1999

Radiation Research

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“…The dose and dose rate could be reduced by redesigning the sample holder and transfer system to avoid the limitations on sample size imposed by the present system. Experiments with larger samples, higher proton energies for greater penetration, and improved EPR detection sensitivity might reveal orientation effects that are not present in our data due to sample heating or other processes that destroy free radicals at high exposure levels (Bernhard, 1981).…”

Section: Discussionmentioning

confidence: 65%

Free-Radical Yields in Proton Irradiation of Oriented DNA: Relationship to Energy Transfer Along DNA Chains

Miller

Frasco

et al. 1993

Biological Effects and Physics of Solar and Galactic Cosmic Radiation

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“…Using a CH H Q value of -72 MHz in the McConnell relation (McConnell and Chesnut, 1958;Bernhard, 1981):…”

Section: The R2 A1 Couplingsmentioning

confidence: 99%

Free radical products in X-irradiated Rochelle salt: low temperature ENDOR and DFT studies

Sagstuen

Hole

Lund

2012

Radiation Physics and Chemistry

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Abstractreactions upon warming. The one-electron gain product was observed and from the hyperfine interaction with a β-proton it was unambiguously centred at the C 4 position of the tartrate moiety. An additional nearly isotropic hyperfine structure of about 21 MHz was tentatively assigned to interaction with a sodium ion exhibiting a close contact to O 3 in the crystal.Evidence was obtained that the one-electron reduced radical had become protonated at one of the C 4 bonded carboxyl oxygens, most probably O 4 . No evidence for the corresponding C 1 -centred reduction product was found. Two resonance lines (R2, A1) were shown by EIE to belong to a species formed by decarboxylation at C 3 , a secondary oxidation product. Two other resonance lines (K1, K2) were assigned to two varieties of another decarboxylation radical, centred at C 2 , distinguished by differences in the potassium ion coordination.Furthermore, one other resonance line (A2) was tentatively ascribed to a third decarboxylation radical, centred at the opposite end of the tartrate moiety. The precursor of these products, that is, the one-electron loss product, was not observed after X-irradiation at 10 K. Thermally induced free radical reactions followed by EPR in the temperature range 12 to 119 K indicate that a water molecule or a hydroxyl ion is eliminated from the one-electron reduction product radical and that a C 3 -centred radical is formed. The reduction and oxidation reaction pathways of hydroxy acid derivatives are discussed.

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Solid-State Radiation Chemistry of DNA: The Bases

Cited by 149 publications

References 146 publications

The Influence of Packing on Free Radical Yields in Solid-State DNA: Film Compared to Lyophilized Frozen Solution

The Influence of Packing on Free Radical Yields in Solid-State DNA: Film Compared to Lyophilized Frozen Solution

Free-Radical Yields in Proton Irradiation of Oriented DNA: Relationship to Energy Transfer Along DNA Chains

Free radical products in X-irradiated Rochelle salt: low temperature ENDOR and DFT studies

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