Solid-state nanopores towards single-molecule DNA sequencing

Capture and translocation of a rod-like molecule by a nanopore: orientation, charge distribution and hydrodynamics

“…The voltage-driven translocation of analytes through a nanopore has attracted a lot of attention due to its potential application to molecular detection in general, 1–3 and DNA gene sequencing 4–6 in particular. In short, an electric field is used to force the analyte through the nanopore (or nanochannel); during this translocation process, the analyte blocks part of the ionic current through the channel.…”

“…A wide range of experimental, theoretical, and computational studies have been conducted to understand the mechanisms of translocation. 6–9…”

DNA sequencing based on electronic tunneling in a gold nanogap: a first-principles study

“…The resulting signal is then decoded to provide the specific DNA sequence. 2,24–27 The parallel use of pores and the ability to repeatedly read a DNA fragment have enabled the nanopore technology to reach single base resolution. 12,25,28 In recent years, it has matured to an extent that it is now being commercialized and used in the industry as a long-read, single base resolution and label-free detection platform.…”

High-yield purification of exceptional-quality, single-molecule DNA substrates

“…RNA molecules can be similarly modified or can be placed between appropriately-modified DNA handles [12,13]. Regardless of whether single molecules of DNA or RNA are being studied, a simple, reproducible method that results in high yields of exceptional quality, nucleic acid substrates is essential [14,15].…”