Solid-State Nanopore Detection of Epigenetic DNA Modifications

Abstract

different pattern sizes and variations in the microstructure, respectively. Here we report on the advantages of PDMS-alternatives for an improved and fast micro-contact printing process. Using those materials we could print proteins in various structures (including pillars, lines and grids) and sizes down to 100 nm. For characterization of pattern quality we used TIRF and super-resolution microscopy and focused on the validation of well-known protein-protein interactions including the one of EGF-, Insulin/IGF1-or betaadrenergic receptors with intracellular binding partners. As TIRF microscopy requires homogenously adhered cells on the micro-patterned surface, we especially focused on the verification of cell adhesion efficiency when using different pattern shapes. An important issue which we are currently addressing is the production of a micro-structured and functionalized multiwell plate (96 and 384 well design). This development step will set a milestone concerning the throughput rates of the micro-patterning assay and increase the number of potential users interested in this methodology.