Solid-State Fermentation with Aspergillus niger GH1 to Enhance Polyphenolic Content and Antioxidative Activity of Castilla Rose (Purshia plicata)

Abstract

This work was performed to study Castilla Rose (Purshia plicata) as a potential source of polyphenols obtained by solid-state fermentation (SSF)-assisted extraction using the microorganism Aspergillus niger GH1 and to evaluate the antioxidant activity of the extracted compounds. First, water absorption capacity (WAC) of the plant material, radial growth of the microorganism, determination of best fermentation conditions, and maximum accumulation time of polyphenols were tested. Then, a larger-scale fermentation, polyphenols isolation by column liquid chromatography (Amberlite XAD-16) and recovered compounds identification by HPLC-MS were made. Finally, the antioxidant activity of the recovered compounds was tested by ABTS, DPPH, and lipid oxidation inhibition assays. The best fermentation conditions were temperature 25 °C and inoculum 2 × 106 spores/g, while the maximum extraction time of polyphenols was 24 h (173.95 mg/g). The HPLC/MS analysis allowed the identification of 25 different polyphenolic compounds, and the antioxidant activity of the obtained polyphenols was demonstrated, showing ABTS assay the most effective with inhibition of 94.34%.

Influence of Ultrasound Application in Fermented Pineapple Peel on Total Phenolic Content and Antioxidant Activity

“…The use of agro-industrial wastes to produce bioactive compounds has been studied in recent years [1][2][3]. Due to the lignocellulosic composition of these substrates, they can be used as sources of macro or micronutrients, and as sources of carbon and nitrogen in fermentation processes [4].…”

“…In recent years organic materials such as Castilla Rose (Purshia plicata), aqueous extracts of wood bio treated with Ceriporiopsis subvermispora and Grape stalks, which have a high lignocellulosic content, have been used as substrates in the SSF. When Castilla Rose is used, it is possible to obtain different functional compounds such as ellagitannins, flavonols, catechins, hydroxycinnamic acids; from grape stalks it is possible to obtain phenolic-oxidizing enzymes such as peroxidases; and finally, from bio-treated wood, several water-soluble phenols are obtained, that exert antioxidant or pro-oxidant effects, converting H2O2 into OH radicals [3,19,20]. In addition, the microorganisms involved in the fermentation process produce enzymes, helping with the extraction and release of these bioactive compounds; and those compounds can also be produced via secondary metabolic pathways [21].…”

Valorization of Mexican Rambutan Peel through the Recovery of Ellagic Acid via Solid-State Fermentation Using a Yeast

“…In a study, Muhtadi et al (2014) [33] evaluated rambutan-peel extracts, employing four different solvents out of which ethyl acetate showed an increase in antioxidant activity. Other polyphenols obtained from an SSF on other kinds of substrates like Castilla rose have shown antioxidant activity; in a study by De León-Medina et al (2020) [34], polyphenols were recovered from Castilla rose using SSF and A. niger. These compounds showed free-radical scavenging activity in ABTS and DPPH with 94.34 and 68.71% inhibition, respectively; this can be directly related to the presence of EA in the polyphenolic extract obtained.…”

Purshia plicata Triggers and Regulates Proteins Related to Apoptosis in HeLa Cancer Cells

“…The same group identified the composition of the extract by high-performance liquid chromatography (HPLC). The most abundant compounds identified in the extract were kaempferol 3,7-Odiglucoside, procyanidin trimer C1, and ellagic acid (Table 1) [12]. Some of the polyphenols found in the extract are listed in Table 1 as well as their families (Table 1).…”

“…While Rosa de Castilla presented a high quantity of bioactive/phytochemical compounds, there is little information on the properties, type, number, and quality of its polyphenol compounds. Early research identified catechin, quercetin (Qu), and ellagic acid as compounds potentially present in Rosa de Castilla [12]. Kaempferol and Qu exhibit potential therapeutic properties against tumour cell lines, but not non-transformed cells, and can enhance apoptosis, induce cell cycle arrest, and reduce reactive oxygen species (ROS), particularly in tumour cells or chemo-resistant cells [3,7,8,13].…”