Solid-State Fermentation vs Submerged Fermentation for the Production of l-Asparaginase

Doriya, Kruthi; Jose, Neethu Maria; Gowda, Manjunath; Kumar, Devarai Santhosh

doi:10.1016/bs.afnr.2016.05.003

Cited by 56 publications

(34 citation statements)

References 57 publications

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“…The evaluation of the production of biocompounds by SSF and SmF has been studied, and certain bioactive compounds have been found to be produced in higher quantities in SSF, whereas other compounds have been extracted using SmF [ 38 ]. Higher production of biomolecules, such as enzymes [ 39 , 40 ] and organic acids [ 41 ], is generally obtained through SSF fermentation systems. However, this fermentation technique and the SSF bioreactors are not easily scaled up.…”

Section: Resultsmentioning

confidence: 99%

Gibberellic Acid Production by Different Fermentation Systems Using Citric Pulp as Substrate/Support

Oliveira

Rodrigues

Vandenberghe

et al. 2017

BioMed Research International

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Gibberellic acid (GA3) is an important phytohormone, a member of gibberellins family, which acts as a promoter and regulator of plant growth. This study aimed to evaluate GA3 production by Fusarium moniliforme LPB03 and Gibberella fujikuroi LPB06 using different techniques of fermentation, solid state fermentation (SSF), submerged fermentation (SmF), and semisolid state fermentation (SSSF), and different types of bioreactors. In all techniques, citric pulp (CP), a subproduct obtained from the extraction of orange juice, was employed as the substrate/support. GA3 production by SSF reached 7.60 g kg−1 and 7.34 g kg−1 in Erlenmeyer flasks and column bioreactors, respectively. For SmF, the highest concentration of GA3 obtained was 236.00 mg L−1 in Erlenmeyer flasks, 273.00 mg L−1 in a 10 L stirred tank reactor (STR), and 203.00 mg L−1 in a 1.5 L bubble column reactor (BCR). SSSF was conducted with a CP suspension. In this case, GA3 concentration reached 331.00 mg L−1 in Erlenmeyer flasks and 208 mg L−1 in a BCR. The choice of the fermentation technique is undoubtedly linked to the characteristics and productivity of each process. The methods studied are inexpensive and were found to produce good proportions of GA3, making them suitable for several applications.

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Section: Resultsmentioning

confidence: 99%

Gibberellic Acid Production by Different Fermentation Systems Using Citric Pulp as Substrate/Support

Oliveira

Rodrigues

Vandenberghe

et al. 2017

BioMed Research International

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“…The commercialized pectinase enzymes are produced by either submerged fermentation (SmF) or solid-state fermentation (SSF) that varies in the use of selected fungus or bacteria ( 20 – 22 ). The SmF technique has been adopted since 1940s when the microorganisms were cultivated on liquid broth with constant agitation for the large-scale production of antibiotics and later widely applied in various industries for the production of a huge assortment of microbial metabolites ( 23 , 24 ). On the other hand, the SSF is also applicable in various sectors for enzyme production where microorganisms are cultured on solid materials which are eco-friendly, cost-effective, and highly productive and have high biomass yield, dwindled proteolytic impact, higher resistance to catabolic repression, better product quality, etc.…”

Section: Production Methods Of Selected Pectinase From Microorganismsmentioning

confidence: 99%

Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing

et al. 2020

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Both pectin and pectinase are vitally imperative biomolecules in the biotechnological sector. These molecules are a feasible non-toxic contrivance of nature with extensive applicative perception. Understanding pectic substances and their structure, unique depolymerization, and biochemical properties such as a catalytic mechanism and the strong interrelationship among these molecules could immensely enhance their applicability in industries. For instance, gaining knowledge with respect to the versatile molecular heterogeneity of the compounds could be considered as the center of concern to resolve the industrial issues from multiple aspects. In the present review, an effort has been made to orchestrate the fundamental information related to structure, depolymerization characteristics, and classification of pectin as well as the types and biochemical properties of pectinase. Furthermore, various production methods related to the optimization of the product and its significant contribution to the pharmaceutical industry (either pectinase or derived pectic substances) are described in this article.

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“…The basic difference between SSF and SmF is the type of substrate that is used, solid in case of SSF and liquid broth in case of SmF, the optimization techniques that can be used to study each of these variations and if possible a better technique to be used can be suggested so as to reduce the redundancy time in the process (Subramaniyam and Vimala 2012;Doriya et al 2016). Evolutionary techniques have the process methodology which includes selection, crossover, and mutation as essential parts of the process.…”

Section: Application Of Optimization Techniques In Ssf and Smfmentioning

confidence: 99%

“…Figure 5c shows the methodology that will be run using ACO technique of optimization where a particular path will be set after multiple experimentations and that path will be used as the optimum for all further runs. These techniques are expected to have a better understanding of the processes associated with SSF; although its applicability in SmF processes cannot be unseen, the amount of time that it takes compared to other techniques that are used to study the SmF process allows these techniques to be limited for the study of SSF process (Roy et al 2006;Doriya et al 2016). SmF has been scaledup to the industrial level and the difficulties that lie with the scaling up of the SSF process makes it important to have a very precise calculation when dealing with the processes involving the SSF studies (Mitchell et al 2000).…”

Section: Swarm Optimizationmentioning

confidence: 99%

Different methodologies for sustainability of optimization techniques used in submerged and solid state fermentation

Ashok

Kumar

2017

3 Biotech

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Optimization techniques are considered as a part of nature's way of adjusting to the changes happening around it. There are different factors that establish the optimum working condition or the production of any value-added product. A model is accepted for a particular process after its sustainability has been verified on a statistical and analytical level. Optimization techniques can be divided into categories as statistical, nature inspired and artificial neural network each with its own benefits and usage in particular cases. A brief introduction about subcategories of different techniques that are available and their computational effectivity will be discussed. The main focus of the study revolves around the applicability of these techniques to any particular operation such as submerged fermentation (SmF) and solid state fermentation (SSF), their ability to produce secondary metabolites and the usefulness in the laboratory and industrial level. Primary studies to determine the enzyme activity of different microorganisms such as bacteria, fungi and yeast will also be discussed. l-Asparaginase, the most commonly used drugs in the treatment of acute lymphoblastic leukemia (ALL) shall be considered as an example, a short discussion on models used in the production by the processes of SmF and SSF will be discussed to understand the optimization techniques that are being dealt. It is expected that this discussion would help in determining the proper technique that can be used in running any optimization process for different purposes, and would help in making these processes less time-consuming with better output.

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Solid-State Fermentation vs Submerged Fermentation for the Production of l-Asparaginase

Cited by 56 publications

References 57 publications

Gibberellic Acid Production by Different Fermentation Systems Using Citric Pulp as Substrate/Support

Gibberellic Acid Production by Different Fermentation Systems Using Citric Pulp as Substrate/Support

Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing

Different methodologies for sustainability of optimization techniques used in submerged and solid state fermentation

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