Solid state fermentation pilot-scaled plug flow bioreactor, using partial least square regression to predict the residence time in a semicontinuous process

Abstract

Semicontinuous production of fungal compounds was obtained with a Plug Flow Bioreactor (PFB) prototype. • Productions of same metabolites obtained over time at lab scale were used to build a Partial Least Square (PLS) model. • A residence time of solid particles was associated to each PFB compartment using the PLS model. • The PFB prototype was suitable to continuously produce fungal secondary metabolites.• PLS was an innovative approach to evaluate the scale-up of the metabolite production. A R T I C L E I N F O Keywords: Plug flow bioreactor Solid state fermentation Partial least square regression Residence time Aspergillus niger A B S T R A C T Plug flow bioreactor (PFB) used in solid state fermentation gives the possibility to have semicontinuous culture.However, it is complicated to follow a single particle during its residence inside the bioreactor and to therefore study precisely the culture process all along the device. In this study, semicontinuous production of fungal compounds was successfully obtained by cultivating Aspergillus niger with a PFB prototype. Kinetic productions of the same metabolites along the bioreactor were obtained at laboratory scale to predict the residence time of the particles inside the PFB from Partial Least Square (PLS). This original methodology allowed (1) the comparison between the production kinetics at the two production scales (pilot and laboratory) and (2) the observation of eventual changes following the scale-up of the process offering a good overall insight of the PFB performance.

Statistical approach to evaluate effect of temperature and moisture content on the production of antioxidant naphtho-gamma-pyrones and hydroxycinnamic acids by Aspergillus tubingensis in solid-state fermentation

“…Indeed, the cultures have been performed in flasks at laboratory scale and it is notorious in SSF; an increase of production scale leads to the formation of important thermic and water gradients in the medium bed that can negatively affect the production [41]. However, encouraging results have been obtained at pilot scale for NγP production [42].…”

Statistical Experimental Design as a New Approach to Optimize a Solid-State Fermentation Substrate for the Production of Spores and Bioactive Compounds from Trichoderma asperellum

“…The cultures were incubated at 29 • C ± 1 • C for 5 days. Flasks were not hermetically closed to allow some oxygen flow by diffusion [4][5][6][7].…”

“…One possible valorization of agro-industrial wastes is solid-state fermentation (SSF). SSF is a biotechnological process where microorganisms are cultured in a solid medium in the absence (or near absence) of free water, although some moisture is present to support the microorganism growth and metabolism [4,5]. Many countries in Africa and Europe have published supporting evidence of the use of SSF in agricultural waste valorization, particularly to induce fungal development that can lead to the formation of secondary metabolites, spore production, and the production of enzymes of industrial interest like cellulases, chitinases, amylases, etc.…”

Biosurfactants: The green generation of speciality chemicals and potential production using Solid-State fermentation (SSF) technology

“…So, it is often necessary to find a compromise between mass and energy transfers qualities and mycelial damages due to agitation. Some studies have reported good productions of spores and enzymes with filamentous fungi belonging to the genera Aspergillus and Trichoderma in agitated SSF systems with lower rotational speedsranging from 1 to 6 rpmand/or intermittent mixings (Carboué et al, 2019;Finkler et al, 2017;Lopez-Ramirez et al, 2018).…”