Solid-state fermentation of groundnut (Arachis hypogaea) shell using Trichoderma sp., tape yeast, and tempeh yeast to produce cellulase

Abduh, Muhammad Yusuf; Ramadhan, Chalil Rizqullah; Fadhlilah, Alfanny Putri; Abdul, Siti Dhiffah Nabilah; Burhan, Khairul Hadi

doi:10.7324/jabb.2022.100421

Cited by 6 publications

(1 citation statement)

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“…Lignocellulolytic biomass waste is commonly used as the readily available substrate or carbon source for fungal fermentation activity. As established by the previous reports, various kinds of biomass waste such as sugarcane bagasse [12], wheat bran [12,13], corn straw [7], groundnut shell [14], and oil palm biomass [8,9,15,16] would result in enzyme production showing different enzymatic activities. The higher cellulose content in oil palm leaves (OPL) (40%) than other biomass such as trunks (34%) and empty fruit bunches (23%) [17] shows its potential to produce cellulolytic and xylanolytic enzymes.…”

Section: Introductionmentioning

confidence: 93%

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Teo¹,

Wahab

2023

J.Trop.Life.Science

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The oil palm biomass produced by plantations and mills in Malaysia is the largest contributor to the nation’s agro-waste, with the oil palm leaves (OPL) topping the list. Nevertheless, the surplus of OPL might have applicability as the substrate for cultivating lignocellulolytic bio-degraders. Ipso facto, this study employed raw OPL as the carbon source for cultivating Trichoderma harzianum under solid-state fermentation (SSF). Optimization of the SSF process using the Taguchi orthogonal design to produce endoglucanase (CMCase) successfully established the optimal fermentation conditions as the following: 7.00 × 108 spore/g inoculum size, 50% moisture content, pH 12 Mandel’s medium, with 3-day incubation at 40°C. The crude enzyme cocktail exhibited the corresponding maximum activity of 417.49 U/g CMCase. The enzymatic activities were significantly affected by factors, moisture content, inoculum size, and initial pH (p-value < 0.05). In short, the high extracellular CMCase activity of the T. harzianum crude enzyme cocktail may prove valuable in accelerating the saccharification of cellulose for biofuel-and nanocellulose production.

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