Solid state fermentation for the synthesis of inulinase from Staphylococcus sp. and Kluyveromyces marxianus

Selvakumar, Ponniah; Pandey, Ashok

doi:10.1016/s0032-9592(99)00008-4

Cited by 98 publications

(49 citation statements)

References 16 publications

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“…The study on the inulinase production by Kluyveromyces marxianus using inulin as substrate obtained 0.47 U/mL of inulinase activity (Selvakumar and Pandey, 1999). Therefore, in this work the inulinase activity optimized achieved 7.3 U/mL using inulin as substrate.…”

Section: Resultsmentioning

confidence: 73%

Parameters optimization for enzymatic assays using experimental design

Burkert

Kalil

Filho

et al. 2006

Braz. J. Chem. Eng.

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-The conditions for maximization enzymatic activity were determined using experimental design and inulinase from Kluyveromyces marxianus ATCC 16045. The effects of substrate concentration (sucrose and inulin), pH and temperature on inulinase activity were verified using four factorial design and surface response analysis. Using sucrose as substrate. It has bean shown that the effects sucrose on enzymatic activity is not statistically significant and the best condition for the highest activity (110 U/mL) was achieved with temperature between 60°C and 68°C and pH between 4.5 and 5.0. Using inulin as substrate it was verified that temperature is the only variable statistically significant and the maximum activity was 7.3 U/mL at temperature between 50°C and 51°C.

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Section: Resultsmentioning

confidence: 73%

Parameters optimization for enzymatic assays using experimental design

Burkert

Kalil

Filho

et al. 2006

Braz. J. Chem. Eng.

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“…Y-85, a mutant of the marine yeast Pichia guilliermondii, Staphylococcus sp. and Kluyveromyces marxianus ATCC 52466 [6][7][8][9][10][11]. However, there was only few reports on inulinases from Streptomyces [12,13].…”

Section: Introductionmentioning

confidence: 99%

Production, Purification and Characterization of Inulinase from a Newly Isolated <i>Streptomyces</i> sp. CP01

Laowklom¹,

Chantanaphan²,

Pinphanichakarn³

2012

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Inulinase is an enzyme catalyzing the hydrolysis of inulin, a plant reserve polysaccharide, into fructoses and fructooligosaccharides which are widely used as food additives. Here we report inulinase from a newly isolated Streptomyces as in the past decade there have been very few reports on inulinases from Streptomyces, especially purification and characterization of these enzymes. Out of 371 Streptomyces isolates, Streptomyces sp. CP01 produced highest inulinase activity of 0.50 U/ml. The enzyme activity was increased to 1.60 U/ml when CP01 was cultivated under the optimal conditions which consisted of using basal medium (Czapek's Dox) containing 1% (w/v) inulin extract from Jerusalem artichoke's root tubers and 0.7% (w/v) tryptone at pH8, shaking at 200 rpm and 28˚C for 24 h. The enzyme was purified from culture filtrate to about 67-fold purity by (NH 4 ) 2 SO 4 precipitation followed by four consecutive column chromatography steps. The purified enzyme is a single peptide with approximate molecular mass of 73 kDa as analyzed by gel filtration and 70.8 kDa as assessed by SDS-PAGE. The enzyme is optimally active at 55˚C and pH 6.0, however it still possesses more than 80% of the maximal activity at pH ranging from 5.5 to 9.0. It is stable at temperature up to 50˚C and at broad range of pH from 5.0 to 9.0 for 30 min. Its K m and V max values for inulin were 2.34 mM and 440 μmol·min -1 ·mg -1 , respectively. This enzyme has potential for industrial application as it is active at moderately high temperature and wide range of pH.

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“…This result is coherent since lower inoculum density cannot produce enough biomass to produce the biomolecules (Sanghi et al 2008;Selvakumar and Pandey 1999). From the PB12 design, the highest herbicidal activity was obtained when fermentation was carried out at 25°C, moisture content of 50 wt%, 10 wt% of corn steep liquor and soybean bran and 15 wt% of inoculum density.…”

Section: Resultsmentioning

confidence: 99%

Solid-state fermentation for production of a bioherbicide from Diaporthe sp. and its formulation to enhance the efficacy

et al. 2017

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In this study, a bioherbicide was produced by solid-state fermentation (SSF) using Diaporthe sp. Adjuvants were employed in a formulation to enhance the herbicidal activity towards the target (Cucumis sativus). The study was divided into two steps: (1) the fermentation condition for bioherbicide production was assessed; (2) evaluation of different formulations containing palm oil, Tween Ò 80 and Span Ò 80, in order to increase phytotoxicity. In step 1, the maximum herbicidal activity (1.23% of the leaves had lesions) was obtained at 25°C, moisture content of 50 wt%, supplemented with 10 wt% of corn steep liquor and soybean bran and inoculum density of 15 wt%. In step 2, the formulation containing 8.2 wt% of palm oil, 8.2 wt% of Tween Ò 80 and Span Ò 80, resulting in an HLB of 12.8 showed the highest phytotoxicity on the leaves. At this condition, dry matter and height of target were reduced about 36% in comparison with control. Diaporthe sp. has the potential to produce molecules with herbicidal activity and the use of adjuvants enhanced three times its efficiency.

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Solid state fermentation for the synthesis of inulinase from Staphylococcus sp. and Kluyveromyces marxianus

Cited by 98 publications

References 16 publications

Parameters optimization for enzymatic assays using experimental design

Parameters optimization for enzymatic assays using experimental design

Production, Purification and Characterization of Inulinase from a Newly Isolated <i>Streptomyces</i> sp. CP01

Solid-state fermentation for production of a bioherbicide from Diaporthe sp. and its formulation to enhance the efficacy

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Solid state fermentation for the synthesis of inulinase from Staphylococcus sp. and Kluyveromyces marxianus

Cited by 98 publications

References 16 publications

Parameters optimization for enzymatic assays using experimental design

Parameters optimization for enzymatic assays using experimental design

Production, Purification and Characterization of Inulinase from a Newly Isolated &lt;i&gt;Streptomyces&lt;/i&gt; sp. CP01

Solid-state fermentation for production of a bioherbicide from Diaporthe sp. and its formulation to enhance the efficacy

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Production, Purification and Characterization of Inulinase from a Newly Isolated <i>Streptomyces</i> sp. CP01