Solid state bioconversion of lignocellulosic residues by Inonotus obliquus for production of cellulolytic enzymes and saccharification

Xu, Xin; Lin, Mengmeng; Zang, Qiang; Shi, Song

doi:10.1016/j.biortech.2017.08.192

Cited by 80 publications

(38 citation statements)

References 34 publications

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“…Most literature data concerning the influence of inoculum concentration on fungal cellulases production by SSF cites the use of spores (Zhang and Sang, 2012;Xu et al, 2018). Therefore, it is difficult to compare these data to the results of the present study.…”

Section: Influence Of the Inoculum Sizementioning

confidence: 62%

Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse

Marques

Pereira

Gomes

et al. 2018

Industrial Crops and Products

110

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A B S T R A C TEndophytic fungi are widely studied as producers of secondary metabolites of biotechnological interest. In recent years, the interest in these fungi as new sources of enzymes, especially hydrolytic, has increased. In the present study, 14 strains of endophytic fungi not yet explored as enzymes sources were randomly chosen and prospected for cellulases and xylanases production by solid-state fermentation. Initially, fungi were cultivated in a mixture (1:1 w/w) of sugarcane bagasse and wheat bran for 7 days, at 28°C. In this initial screening, 4 fungi excelled in endoglucanase activity (U/g): Cladosporium cladosporioides PAJ 03 (88.51 ± 1.0), Phomopsis stipata SC 04 (83.44 ± 7.7), Trichoderma viridae PAJ 01 (64.56 ± 4.0) and Botryosphaeria sp. AM 01 (42.79 ± 1.6). On the other hand, the following 4 fungi stood out in relation to β-glucosidase activity (U/g): Saccharicola sp. EJC 04 (51.56 ± 2.7), Paecilomyces sp. SF 021 (33.19 ± 9.2), Ustilaginoidea sp. CV 04 (29.75 ± 0.8) and Ustilaginoidea sp. XYA 04 (21.72 ± 3.05). Among these fungi, P. stipata SC 04 and Botryosphaeria sp. AM 01 were the best producers of xylanase and β-xilosidase (694,33 and 4,87 U/g, respectively). These 8 fungi were then cultured in new mixtures (1:1 w/w) of lignocellulosic substrates. Botryosphaeria sp. AM01and Saccharicola sp. EJC04 stood out regarding endoglucanase and β-glucosidase activities (184.74 ± 6.0 and 92.28 ± 9.57 U/g, respectively) when cultivated on cotton seed meal and wheat bran and were selected to continue the study. The influence of time cultivation, inoculum amount and substrate initial moisture content was evaluated and the best condition for cellulases production was 192 h, six mycelial plugs and 65%, respectively, for both fungi. Cellulases and xylanases produced under these conditions were characterized and optimum pH and temperature values were between 4.5-6 and 60-75°C, respectively. The enzymes were stable over a wide pH range and under 30-70°C. β-glucosidase from both isolates retained about 75-80% of their activity in the presence of glucose at 6 mM. The presence of ethanol stimulated β-glucosidase activity from Botryosphaeria sp. AM01 (about 60% higher in the presence of ethanol at 15%). On the other hand, the activity of β-glucosidase produced by Saccharicola sp. EJC 04 was reduced at ethanol concentrations above 15%. A blend of the enzymatic extracts was used to saccharify pretreated sugarcane bagasse and a face-centered central composite design was used to find the best conditions. Under the predicted optimum condition (50°C, 5% of sugarcane bagasse, 150 U g −1 of endoglucanase and 20 h), glucose and xylose concentrations obtained were 3.56 and 1.66 mg mL −1 , respectively. These results show that the 14 endophytic fungi studied have potential to be explored as producers of plant material degrading enzymes. Botryosphaeria sp. AM01 and Saccharicola sp. EJC 04 are promising in relation

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Section: Influence Of the Inoculum Sizementioning

confidence: 62%

Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse

Marques

Pereira

Gomes

et al. 2018

Industrial Crops and Products

110

View full text Add to dashboard Cite

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“…Previous reports showed that soft rot fungi, such as Aspergillus niger and Trichoderma reesei (Xue et al 2017), and the white rot fungus Phanerochaete chrysosporium produce large amounts of cellulase (Manavalan et al 2015). A recent study showed that some of the white rot fungi are the most promising fungi in terms of biomass degradation and delignification because of their potential to synthesize cellulolytic, hemicellulolytic, and ligninolytic enzymes (Xu et al 2018). Hydrolysis zone diameter was correlated with colony diameter on CMC and xylan agar media.…”

Section: Screening Of Cellulase and Xylanase Activitymentioning

confidence: 91%

Characterization of thermophilic fungi producing extracellular lignocellulolytic enzymes for lignocellulosic hydrolysis under solid-state fermentation

Saroj

Manasa

Narasimhulu

2018

Bioresour. Bioprocess.

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Background: Thermotolerant lignocellulolytic enzymes have become a subject of interest in industrial processes due to their ability to degrade lignocellulosic polysaccharides. Development of cost-effective, large-scale screening for production of desirable enzymes by thermophilic fungi is a challenge. The present investigation focused on isolating, screening, and identifying industrially relevant thermophilic producers of lignocellulolytic enzymes from various locations in the Warangal district, Telangana, India. Results: Fifteen thermophilic fungi were isolated from soil on their ability to grow at 50 °C and were screened for their activity of cellulase, hemicellulase, and lignin degradation based on holo zone around colonies. The appearance of the black color zone of diffusion in esculin agar is a positive indication for the β-glucosidases activity test. Out of fifteen isolates, Aspergillus fumigatus JCM 10253 have shown as a potential producer of extracellular enzymes for lignocelluloses degradation showing higher activity for cellulase (EI 1.50) as well as β-glucosidase (4 mg/mL), simultaneously for xylanase (EI 1.18) by plate assay methods. A. fumigatus JCM 10253 was selected for extracellular hydrolytic enzymes production under solid-state fermentation. Maximum CMCase (26.2 IU/mL), FPase (18.2 IU/mL), β-glucosidase (0.87 IU/mL), and xylanase (2.6 IU/mL) activities were obtained after incubation time of 144 h at 50 °C. The thermostability of crude cellulase showed the optimum activity at 60 °C and for FPase, β-glucosidase, and xylanase at 50 °C which recommended that the enzymes have a potentially significant role in the biofuel industries. Conclusion: The high titer production of active enzymes that cleave different β-1,4-glycosidic bonds still remains a challenge and is the major bottleneck for the lignocellulosic conversion. In particular, the finding of thermostable enzymes which would allow the development of more robust processes is a major goal in this field.

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“…In fact, the GH enzyme family is the enzyme of choice to degrade biomass. The process is simple in which the biomass serves as the main carbon source for microbial growth [30][31][32]. The result of the fermentation process produces sugars and then proceeds to enzymatic saccharification to yield bioethanol [31,33].…”

Section: Cellulosementioning

confidence: 99%

On the Renewable Polymers from Agro-industrial Biomass: A Mini Review

Wahab

Gowon²,

Elias

2019

J. Idn. Chem. Soc.

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Plant biomass is the most abundant natural resources on earth. However, current strategies for the utilization of agricultural biomass is far from efficient, thus environmental issues related to incompetent management of biomass prevail. Innovative handling of surplus biomass can yield several rewards, which includes renewability and sustainability of the commodity as feedstock for industrial and energy purposes. In fact, an array of different parts of a plant or agro-industrial biomass, for instance, shell, husks, wood, and leaves were successfully converted into advanced carbon materials, for use as absorbent, catalyst enzyme support, electrode, etc. In this review, an extensive literature survey related to areas of renewable sources of biopolymer in both the agricultural and industrial sectors were performed. Information on their industrial value and uses, the fundamentals of their extraction alongside the benefits and major drawbacks of their utilization, are also highlighted. We aim to show that the smart utilization of unwanted agro-industrial biomass encompasses a portion of a bigger scheme that intelligently uses biomass to complement current agricultural advancements that create smart crops and growing them using cleverly designed technology. The best part of this “Waste to Wealth” concept is that every part of the crop is fully utilized. However, a set of clear criteria must be in place to ensure a sustained momentum, so that the green approach of responsible biomass utilization will be fully embraced by nations worldwide.

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Solid state bioconversion of lignocellulosic residues by Inonotus obliquus for production of cellulolytic enzymes and saccharification

Cited by 80 publications

References 34 publications

Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse

Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse

Characterization of thermophilic fungi producing extracellular lignocellulolytic enzymes for lignocellulosic hydrolysis under solid-state fermentation

On the Renewable Polymers from Agro-industrial Biomass: A Mini Review

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