Solid state 13C NMR analysis of human gallstones from cancer and benign gall bladder diseases

Jayalakshmi, K.; Sonkar, Kanchan; Behari, Anu; Kapoor, Vinay K.; Sinha, Neeraj

doi:10.1016/j.ssnmr.2009.06.001

Cited by 21 publications

(14 citation statements)

References 29 publications

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“…Precipitation of cholesterol from supersaturated bile leads to the gallstone formation and is related to several different pathways, which in turn include nucleation, crystallization, growth, and agglomeration of cholesterol crystals. [7] The fourth gallstone is approximately 3 cm long with a pale color and a pebble-like appearance. An ideal concentric structure is shown in the crosssection (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…[6] Such analytical studies, incorporating both nondestructive and destructive analytical methodologies, have been conducted worldwide, providing significant information on the formation and composition of gallbladder stones and helping physicians to take prophylactic measures for the patients. [2,3,5] Following the classification of gallbladder stones by Grunhage and Lammert, [5] there exist yellowcolored pure cholesterol stones, black pigment stones, and brown pigment stones, while Jayalakshmi et al [7] refer to three morphological classes: cholesterol, pigment, and mixed stones. Considering the above-mentioned categorization, this study focuses on characteristic bladder calculi with distinctive morphology and structure.…”

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confidence: 99%

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Characterization of Gallbladder Stones From Cholelithiasis Patients of Northern Greece, Using Complementary Techniques

Iordanidis

García-Guinea

Giousef

et al. 2013

Spectroscopy Letters

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The present study aims to characterize gallbladder stones surgically removed from patients dwelling in northern Greece. Combined techniques were employed, such as environmental scanning electron microscopy coupled with an energy dispersive system, l Raman spectroscopy, X-ray diffraction, and cathodoluminescence for the determination of morphological, microstructural, and compositional (chemical and mineralogical) characteristics. Four characteristic gallstones were determined: a black pigment, consisting of aragonite and bilirubin; a mixed stone, composed of calcite, oxalates, and phosphates; a cholesterol-rich stone; and a large stone, consisting of uric acid. The importance of using such mineralogical complementary techniques is emphasized in this work. It should be noted that this is the first published analytical characterization of gallbladder stones from northern Greece.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Characterization of Gallbladder Stones From Cholelithiasis Patients of Northern Greece, Using Complementary Techniques

Iordanidis

García-Guinea

Giousef

et al. 2013

Spectroscopy Letters

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“…Elevated level of cholesterol, Ca and Mg in GS was also reported in NMR based study performed by Srivastava et al [11]. In yet another, analysis by NMR have characterized that cholesterol polymorphs was found to be different in different GBC samples [12]. One study executed by FTIR, found mainly cholesterol and bilirubin as major component in GS samples.…”

Section: Resultsmentioning

confidence: 63%

Gallstones: A Worldwide Multifaceted Disease and Its Correlations with Gallbladder Carcinoma

et al. 2016

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BackgroundGallstones (GS) associated diseases are among the most recurrent and frequent diseases delineated in India and United Arab Emirates. Several reports suggest that the association of GS with gallbladder cancer (GBC) is very high in Northern part of India; however, its occurrence in UAE and Southern part of India is notably low. Therefore, in the present study, we aimed to perform compositional analysis of GS in three different geographical areas by Solid State Nuclear Magnetic Resonance and Fourier Transformed Infrared spectroscopy.MethodsNatural abundance 13C cross polarization magic angle spinning Nuclear Magnetic Resonance and Fourier Transform Infrared spectroscopy is employed for the analysis of human gallstone.ResultsCholesterol, bilirubin and calcium carbonate were present in variant concentrations in GS obtained from three different geographical regions. Cholesterol was present predominantly in gallstones from North India. Bilirubin was found to be a main constituent in gallstones pertaining to South India. Whereas GS from UAE showed both cholesterol and bilirubin as their major constituents. Calcium carbonate was found in varying concentrations in gallstones acquired from different regions.ConclusionVariation in environmental condition and dietary habits may contribute and affect the GS formation. Alterations in bile composition influence the GB and augment the crystallization of cholesterol. Analysis of different geographical regions GS could be an important stride to understand the etiology of GS diseases.

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“…The XRD pattern of the same sample provided information of a mixture of cholesterol crystalline structures (characteristic peaks of d-spacing at 5.21, 4.11 and 4.89 Å) and organic-rich phases, most probably assigned to oxalic acids (peak at 5.56 Å). Precipitation of cholesterol from supersaturated bile leads to the gallstone formation and is related to several different pathways, which in turn include nucleation, crystallization, growth and agglomeration of cholesterol crystals (Jayalakshmi et al, 2009;Athanasiadou et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Analytical geochemistry in the service of medicine: An experimental study of urinary stones from Northern Greece

Iordanidis¹,

García-Guinea

2017

geosociety

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Knowledge of the precise human biomineral composition may allow physicians to recommend an appropriate prophylactic therapy for the patient and thus prevent or delay the stone recurrence. The present study focuses on the application of complementary analytical techniques to the characterization of human urinary stones. Several gallbladder and renal stone samples were obtained from patients dwelling in areas of northern Greece. A comprehensive analytical study took place, employing the following, common in analytical geochemistry, techniques: Environmental Scanning Electron Microscopy (ESEM) coupled to Energy Dispersive System (EDS), X-Ray Diffraction (XRD), thermogravimetry (TG), μRaman spectroscopy and Cathodoluminescence (CL). A detailed determination of morphological, micro-structural, molecular, chemical and mineralogical characteristics of the urinary stone samples was achieved. It was evident by our study the application of powerful analytical techniques could substantially help the medical advisors to ascribe a medical treatment of diseases related to stone formation.

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Solid state 13C NMR analysis of human gallstones from cancer and benign gall bladder diseases

Cited by 21 publications

References 29 publications

Characterization of Gallbladder Stones From Cholelithiasis Patients of Northern Greece, Using Complementary Techniques

Characterization of Gallbladder Stones From Cholelithiasis Patients of Northern Greece, Using Complementary Techniques

Gallstones: A Worldwide Multifaceted Disease and Its Correlations with Gallbladder Carcinoma

Analytical geochemistry in the service of medicine: An experimental study of urinary stones from Northern Greece

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