Solid phase synthesis of a thrombin binding aptamer on macroporous silica for label free optical quantification of thrombin

Terracciano, Monica; Stefano, Luca De; Borbone, Nicola; Politi, Jane; Oliviero, Giorgia; Nici, Fabrizia; Casalino, Maurizio; Piccialli, Gennaro; Dardano, Principia; Varra, Michela; Rea, Ilaria

doi:10.1039/c6ra18401d

Cited by 41 publications

(37 citation statements)

References 59 publications

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“…The first parameter we examine is the capture probe surface density, which is considered of high importance for surface-based biosensors, in general, 77 − 80 and PSi-based biosensors, in particular. 22 , 23 , 81 , 82 Although for both planar and porous surfaces, an overall similar capture probe density may be attained, the large surface area of PSi allows immobilizing larger amounts of capture probes and their concentration within the nanoscale pores. This in turn reduces the binding time, affects the apparent off rate of the probe–analyte complexes, and enhances the biosensor sensitivity, as has been previously suggested.…”

Section: Resultsmentioning

confidence: 99%

Mass Transfer Limitations of Porous Silicon-Based Biosensors for Protein Detection

et al. 2020

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Porous silicon (PSi) thin films have been widely studied for biosensing applications, enabling label-free optical detection of numerous targets. The large surface area of these biosensors has been commonly recognized as one of the main advantages of the PSi nanostructure. However, in practice, without application of signal amplification strategies, PSi-based biosensors suffer from limited sensitivity, compared to planar counterparts. Using a theoretical model, which describes the complex mass transport phenomena and reaction kinetics in these porous nanomaterials, we reveal that the interrelated effect of bulk and hindered diffusion is the main limiting factor of PSi-based biosensors. Thus, without significantly accelerating the mass transport to and within the nanostructure, the target capture performance of these biosensors would be comparable, regardless of the nature of the capture probe–target pair. We use our model to investigate the effect of various structural and biosensor characteristics on the capture performance of such biosensors and suggest rules of thumb for their optimization.

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Section: Resultsmentioning

confidence: 99%

Mass Transfer Limitations of Porous Silicon-Based Biosensors for Protein Detection

et al. 2020

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“…Before the etching process, the silicon substrate was immersed in HF solution for 2 min to remove the oxide native layer. A current density of 20 mA cm −2 for 90 s, was applied to obtain a single layer of PSi with a porosity of 61% (n PSi = 1.83 at λ = 1.2 μm), a thickness L of 2.1 μm and a pores dimension between 50 and 250 nm, determined by ellipsometry and SEM imaging (Terracciano et al, 2016 ). The as-etched PSi was placed in a Schlenk tube containing deoxygenated neat UDA (99% v/v) and allowed to react at 110°C for 18 h under a stream of argon.…”

Section: Methodsmentioning

confidence: 99%

Toward Multi-Parametric Porous Silicon Transducers Based on Covalent Grafting of Graphene Oxide for Biosensing Applications

Moretta¹,

Terracciano

Dardano

et al. 2018

Front. Chem.

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Graphene oxide (GO) is a two-dimensional material with peculiar photoluminescence emission and good dispersion in water, that make it an useful platform for the development of label-free optical biosensors. In this study, a GO-porous silicon (PSi) hybrid device is realized using a covalent chemical approach in order to obtain a stable support for biosensing applications. Protein A, used as bioprobe for biosensing purposes, is covalently linked to the GO, using the functional groups on its surface, by carbodiimide chemistry. Protein A bioconjugation to GO-PSi hybrid device is investigated by atomic force microscopy (AFM), scanning electron microscopy (SEM), water contact angle (WCA) measurements, Fourier transform infrared (FTIR) spectroscopy, steady-state photoluminescence (PL), and fluorescence confocal microscopy. PSi reflectance and GO photoluminescence changes can thus be simultaneously exploited for monitoring biomolecule interactions as in a multi-parametric hybrid biosensing device.

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“…Some electron microscopy images are compared in Figure . Macroporous silica, obtained by electrochemical etching of crystalline silicon followed by a thermal oxidation at 900 °C, was demonstrated as platform for in situ synthesis of Thrombin Binding Aptamer for optical biosensing purpose: its morphology is surprisingly similar to that of diatom Coscinodiscus wailesii , as shown in Figures a,b . Figures c,d report SEM images of a chirped photonic‐crystal fiber and the valve of diatom Arachodiscus wailesii , respectively; both the structures have the property to focus the light .…”

Section: Bioderived Nanostructured Silicamentioning

confidence: 99%

Section: Bioderived Nanostructured Silicamentioning

confidence: 99%

Synthetic vs Natural: Diatoms Bioderived Porous Materials for the Next Generation of Healthcare Nanodevices

Rea¹,

Terracciano²,

Stefano³

2016

Adv Healthcare Materials

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Nanostructured porous materials promise a next generation of innovative devices for healthcare and biomedical applications. The fabrication of such materials generally requires complex synthesis procedures, not always available in laboratories or sustainable in industries, and has adverse environmental impact. Nanosized porous materials can also be obtained from natural resources, which are an attractive alternative approach to man-made fabrication. Biogenic nanoporous silica from diatoms, and diatomaceous earths, constitutes largely available, low-cost reservoir of mesoporous nanodevices that can be engineered for theranostic applications, ranging from subcellular imaging to drug delivery. In this progress report, main experiences on nature-derived nanoparticles with healthcare and biomedical functionalities are reviewed and critically analyzed in search of a new collection of biocompatible porous nanomaterials.

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Solid phase synthesis of a thrombin binding aptamer on macroporous silica for label free optical quantification of thrombin

Cited by 41 publications

References 59 publications

Mass Transfer Limitations of Porous Silicon-Based Biosensors for Protein Detection

Mass Transfer Limitations of Porous Silicon-Based Biosensors for Protein Detection

Toward Multi-Parametric Porous Silicon Transducers Based on Covalent Grafting of Graphene Oxide for Biosensing Applications

Synthetic vs Natural: Diatoms Bioderived Porous Materials for the Next Generation of Healthcare Nanodevices

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