Solid-phase polymerase chain reaction: applications for direct detection of enteric pathogens in waters

Toranzos, Gary A.; Alvarez, Abdiel J.

doi:10.1139/m92-062

Cited by 23 publications

(17 citation statements)

References 18 publications

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“…Bej et al (1991a) reported that PCR was not inhibited by the presence of PTFE and polyvinylidene difluoride filters, with PTFE giving the greatest sensitivity, but was inhibited by polycarbonate, nitrocellulose, and cellulose acetate filters. Both Nytran (Alvarez et al 1994) and nitrocellulose (Toranzos and Alvarez 1992) filters have been successfully used in solidphase PCR, where cell lysis and PCR amplification are performed on the membrane.…”

Section: Concentration and Filter Elutionmentioning

confidence: 99%

Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

Dungan

Leytem

2009

World J Microbiol Biotechnol

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The generation of airborne microorganisms from concentrated animal-feeding operations (CAFOs) is a concern from a human and animal health perspective. To better understand the airborne microorganisms found in these environments, a number of collection and analytical techniques have been utilized and will be discussed in this review. The most commonly used bioaerosol collection method is the liquid impingement format, which is suitable with a number of culture-based and non-culture molecularbased approaches, such as polymerase chain reaction. However, the vast majority of airborne microorganism studies conducted at CAFOs utilize culture-based analyses. Because of the limitations often associated with culturebased analyses, we focused our discussion on the application of molecular-based techniques to identify and/or quantify microorganisms, as they have promising application in bioaerosol research. The ability to rapidly characterize airborne microorganisms will help to ensure protection of public and environmental health.

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Section: Concentration and Filter Elutionmentioning

confidence: 99%

Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

Dungan

Leytem

2009

World J Microbiol Biotechnol

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“…Microparticles come in all shapes and sizes, from soft spheres to porous structures to irregularly shaped rocks, in paramagnetic or non-magnetic varieties. Relative to the entire bio/chemical process, however, we find that microparticles are also compatible with each of the functions outlined in Figure 1: whole cell concentration (29,30), cell lysis (31), nucleic acid purification (32), solidphase PCR amplification (33), and detection (34)(35)(36). This is not to say that a particle (or more generally, a reactive surface) is required to perform each of the functions involved in any particular biodetection process, only that many of the selective chemistries and functional attributes required to complete innumerable biodetection processes are compatible with microparticle-based systems and selective-chemistry.…”

Section: Bio/chemistrymentioning

confidence: 76%

Advances Towards Integrated Biodetection Systems for Environmental Molecular Microbiology

Chandler

2002

Current Issues in Molecular Biology

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To overcome many of the limitations associated with indirect detection methods, new techniques for the sensitive, specific, and direct detection of nucleic acids are required in order to accurately and quantitatively ascribe phenotype/function to uncultivated microorganisms. However, if advanced diagnostic and detection systems are going to be applied in environmental microbiology, future "biodetection" technologies and systems must be developed not from the point of view of the detector, but from the unique aspects of the environmental sample and the entire analytical process. This article highlights recent advances in nucleic acid-based technologies, and looks towards future advances that may address the broad needs and conditions imposed by environmental molecular microbiology.

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“…154,155 In recent years, the use of immunomagnetic beads attached to specific antibodies have become a popular approach in facilitating the capture, concentration, and purification of target bacteria prior to DNA extraction. 156 Several assays based on the direct detection of indicator and pathogenic bacterial cells in environmental water samples by filtration and PCR [157][158][159][160] have been developed. The use of combinations of methods such as immunomagnetic capturing of strains, PCR, and detection of immobilized amplified nucleic acids by hybridization have been proven to be useful for the detection of waterborne pathogenic bacteria that are difficult to culture from environmental sources, for example, Y. enterocolitica, 161,162 and H. pylori.…”

Section: Nucleic Acid-based Detection Methodsmentioning

confidence: 99%

Emerging Waterborne Infections: Contributing Factors, Agents, and Detection Tools

Theron

Cloete

2002

Critical Reviews in Microbiology

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Because microorganisms are easily dispersed, display physiological diversity, and tolerate extreme conditions, they are ubiquitous and may contaminate and grow in water. The presence of waterborne enteric pathogens (bacteria, viruses, and protozoa) in domestic water supplies represents a potentially significant human health risk. Even though major outbreaks of waterborne disease are comparatively rare, there is substantial evidence that human enteric pathogens that are frequently present in domestic water supplies are responsible for low-level incidence of waterborne microbial disease. Although these diseases are rarely debilitating to healthy adults for more than a few hours to a few days, enteric pathogens can cause severe illness, even death, for young children, the elderly, or those with compromised immune systems. As the epidemiology of waterborne diseases is changing, there is a growing global public health concern about new and reemerging infectious diseases that are occurring through a complex interaction of social, economic, evolutionary, and ecological factors. New microbial pathogens have emerged, and some have spread worldwide. Alternative testing strategies for waterborne diseases should significantly improve the ability to detect and control the causative pathogenic agents. In this article, we provide an overview of the current state of knowledge of waterborne microbial pathogens, their detection, and the future of new methods in controlling these infectious agents.

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Solid-phase polymerase chain reaction: applications for direct detection of enteric pathogens in waters

Cited by 23 publications

References 18 publications

Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

Advances Towards Integrated Biodetection Systems for Environmental Molecular Microbiology

Emerging Waterborne Infections: Contributing Factors, Agents, and Detection Tools

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