Solid-phase microextraction with on-fibre derivatisation applied to the analysis of volatile carbonyl compounds

Stashenko, Elena; Puertas, Miguel A.; Salgar, William; Delgado, Wilman; Martı́nez, Jairo René

doi:10.1016/s0021-9673(00)00479-9

Cited by 75 publications

(48 citation statements)

References 18 publications

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“…A similar result has been reported by Stashnko et al using SPME 10) . In conclusion, the use of the SPME fiber and a gas chromatograph equipped with a septum-free injector would be useful for the analysis of aldehydes as an indicator of oxidation and as a biomarker for disease.…”

supporting

confidence: 91%

“…It has been reported that both the affinity of aldehydes for the headspace and the ability of aldehydes to access the SPME fiber coating affect the aldehyde peak area 9) . In the GC analysis of the 2,4-dinitrophenylhydradine derivatives of low-molecular (C 1 -C 10 ) aldehydes 10) , hexanal showed the largest peak area, followed by pentanal, butanal, nonanal, propanal, and decanal. The different peak areas found in this study ( ) would depend on the different affinities of the aldehydes for the SPME fiber coating.…”

mentioning

confidence: 99%

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A Facile Method for the Detection of Aldehydes in Oxidized Lipids Using Solid-Phase Microextraction Fiber and Gas Chromatograph Equipped with a Septum-Free Injector

et al. 2009

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A facile method is described for the detection of volatile odor components from oxidized lipids. The method is based on headspace solid-phase microextraction (HS-SPME) of the compounds in the vial head space and the subsequent analysis of the volatiles by gas chromatography (GC) using a septum-free sample injector. The extraction was done using a 85 mm carboxen-poly(dimethylsiloxane) SPME fiber and the volatiles were desorbed onto a GC instrument with the septum-free injection port. The septum free injector made it possible to analyze the samples without using a conventional silicone rubber septum, that is easily damaged by the repetitive stubs of a relatively thick blunt needle into the septum and a long desorption time after injection. The method was standardized with authentic C 3 -C 10 aldehydes and was applied to the detection of aldehyde components from oxidized fish oil. Several volatiles, including propanal and hexanal, were clearly separated on a polar capillary column (60 m) with reproducible retention times. The present study demonstrates that the method using the GC septum-free injector and the HS-SPME fiber would be useful for the determination of volatile components derived from edible oils.

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confidence: 91%

mentioning

confidence: 99%

A Facile Method for the Detection of Aldehydes in Oxidized Lipids Using Solid-Phase Microextraction Fiber and Gas Chromatograph Equipped with a Septum-Free Injector

et al. 2009

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“…The volatiles were isolated by in vivo HS-SPME (PDMS/DVB, 65 lm), placing the fiber (30 min) into the open flower without touching flower walls or pestle. In vivo HS-SPME/GC-MS analysis of flower volatiles was performed with the fiber (PDMS, 100 lm) previously saturated with pentafluorophenylhydrazine (PFPH), used as an aldehyde and ketone derivatizing agent [87], in order to selectively detect some volatile aliphatic aldehydes [88], present as traces in the B. suaveolens flower scent (barely detected by HS-SPME with mass spectrometric or flame ionization detection). The determination of aldehydes and ketones, as PFPH-derivatives, was performed by capillary GC with electron capture detection (ECD), as described elsewhere [89].…”

Section: Example Of Hs-spme and On-fiber Derivatizationmentioning

confidence: 99%

Sampling flower scent for chromatographic analysis

Stashenko¹,

Martı́nez

2008

J of Separation Science

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The analysis of flower volatiles requires special methods for their isolation with enrichment. Living flowers show a continuous change in their volatile profile that depends on intrinsic (genetic) and external (light, temperature, hydric stress) factors. Excised flowers suffer rapid deterioration and loss of volatiles. While industrial isolation methods for flower volatiles are well established, those at the laboratory-scale experience progressive development, in the search for higher sensitivity, reproducibility, and simplicity. This review covers the flower scent sampling methods most commonly employed during the last decade, and includes comments on their strengths and limitations. The strengths of headspace solid-phase microextraction (HS-SPME) for in vivo monitoring are emphasized with the examples of monitoring the circadian variation of Brugmansia suaveolens flower scent and of volatile aldehyde detection in flower scent using on-fiber derivatization.

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“…Solid-phase microextraction SPME is an alternative extraction technique that combines sampling and sample preparation in one step, which was invented by Pawliszyn 12 . SPME is widely applied in analytical practice, and it has many advantages that include simplicity of operation, a short extraction time, solvent-free operation, the possibility of automation, straightforward linkup with GC, and relatively good results of the isolation of trace amounts of analytes 13 . The headspace SPME HS-SPME analysis provides an actual sensory, that sampling of the volatiles was fast and easy way been widely applied to many types of food material 14 19 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of Volatile Compounds with HS-SPME from Oxidized n-3 PUFA Rich Oils via Rancimat Tests

et al. 2017

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Solid-phase microextraction with on-fibre derivatisation applied to the analysis of volatile carbonyl compounds

Cited by 75 publications

References 18 publications

A Facile Method for the Detection of Aldehydes in Oxidized Lipids Using Solid-Phase Microextraction Fiber and Gas Chromatograph Equipped with a Septum-Free Injector

A Facile Method for the Detection of Aldehydes in Oxidized Lipids Using Solid-Phase Microextraction Fiber and Gas Chromatograph Equipped with a Septum-Free Injector

Sampling flower scent for chromatographic analysis

Characterization of Volatile Compounds with HS-SPME from Oxidized n-3 PUFA Rich Oils via Rancimat Tests

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