Solid-Phase Microextraction (SPME) Technique for Measurement of Generation of Fresh Cucumber Flavor Compounds

Abstract

Investigations were carried out to determine whether flavor compounds characteristic for fresh cucumbers could be rapidly determined using a solid-phase microextraction (SPME) dynamic headspace sampling method combined with gas chromatography and flame ionization detection. Cucumbers were sampled, during blending, for fresh cucumber flavor compounds (E,Z)-2,6-nonadienal and (E)-2-nonenal. The GC was such that the two target compounds were separated and baseline-resolved. Relative standard deviations for analysis of both (E,Z)-2,6-nonadienal and (E)-2-nonenal using this SPME sampling method were +/-10%. Utility of the analytical method was demonstrated by determining the effect of heat treatments on the ability of cucumbers to produce these flavor impact compounds.

Fresh Cucumber Flavor in Refrigerated Pickles: Comparison of Sensory and Instrumental Analysis

“…The odor threshold for (E,Z)-2,6-nonadienal has been estimated to be 0.01 ppb (5), whereas the threshold for (E)-2-nonenal is somewhat higher at about 0.08 ppb (6). These extremely low thresholds are several 100,000-fold lower than the amount present when fresh cucumbers are blended (7,8). The olfactory sense has trouble accommodating a 100-fold difference between the threshold and the concentration which saturates the receptor (9).…”

Volatile compounds in the peel and flesh of cucumber (Cucumis sativusL.) grafted onto bottle gourd (Lagenaria siceraria) rootstocks

“…Currently, it is believed that the unsaturated aldehydes (E,Z)-2,6-nonadienal and (E)-2-nonenal are the most important VOCs in cucumber, since they are the most abundant VOCs (Kemp et al, 1974;Buescher and Buescher, 2001;Palma-Harris et al, 2001). (E,Z)-2,6-Nonadienal is known as the "cucumber aldehyde" due to its unique odour and relatively high abundance in cucumber (Schieberle et al, 1990).…”

Changes of volatiles' attribute in durian pulp during freeze- and spray-drying process

“…To achieve the greatest accuracy, volatiles' quantification was conducted using standard addition method which modified from previous works (Lambropoulou & Albanis, 2002;Palma-Harris, McFeeters, & Fleming, 2001). Devices including a manual SPME sampling unit and SPME fiber with 50/ 30 mm divinylbenzene/Carboxen on polydimethylsiloxane coating (Supelco, Bellefonte, PA) were applied.…”