Solid phase microextraction coupled to mass spectrometry<i>via</i>a microfluidic open interface for rapid therapeutic drug monitoring

Looby, Nikita; Tascón, Marcos; Acquaro, Vinicius Ricardo; Reyes‐Garcés, Nathaly; Vasiljević, Tijana; Gómez‐Ríos, Germán Augusto; Wąsowicz, Marcin; Pawliszyn, Janusz

doi:10.1039/c9an00041k

Cited by 30 publications

(15 citation statements)

References 31 publications

(55 reference statements)

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“…With regard to instrumental analysis, there are many options for the direct coupling of the SPME probe with analytical instrumentation, particularly mass spectrometers [ 61 ]. A strategy which seems to be specifically suitable for this purpose is the microfluidic open interface, presented lately for the fast monitoring of drugs [ 62 , 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Phenotyping of Gliomas: What Can We Get with Simplified Protocol for Intact Tissue Analysis?

Goryńska

Chmara

Kupcewicz

et al. 2022

Cancers

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Glioblastoma multiforme is one of the most malignant neoplasms among humans in their third and fourth decades of life, which is evidenced by short patient survival times and rapid tumor-cell proliferation after radiation and chemotherapy. At present, the diagnosis of gliomas and decisions related to therapeutic strategies are based on genetic testing and histological analysis of the tumor, with molecular biomarkers still being sought to complement the diagnostic panel. This work aims to enable the metabolomic characterization of cancer tissue and the discovery of potential biomarkers via high-resolution mass spectrometry coupled to liquid chromatography and a solvent-free sampling protocol that uses a microprobe to extract metabolites directly from intact tumors. The metabolomic analyses were performed independently from genetic and histological testing and at a later time. Despite the small cohort analyzed in this study, the results indicated that the proposed method is able to identify metabolites associated with different malignancy grades of glioma, as well as IDH and 1p19q codeletion mutations. A comparison of the constellation of identified metabolites and the results of standard tests indicated the validity of using the characterization of one comprehensive tumor phenotype as a reflection of all diagnostically meaningful information. Due to its simplicity, the proposed analytical approach was verified as being compatible with a surgical environment and applicable for large-scale studies.

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Section: Discussionmentioning

confidence: 99%

Metabolomic Phenotyping of Gliomas: What Can We Get with Simplified Protocol for Intact Tissue Analysis?

Goryńska

Chmara

Kupcewicz

et al. 2022

Cancers

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“…The obtained data were then analyzed in the view of their biological significance and compared with up-to-date literature reports to verify findings. It needs to be emphasized here that the instrumental analysis was still performed in the university laboratory; however, the presented sampling approach can be combined directly with a mass spectrometer or other detector for fast quantitative analysis [20][21][22][23].…”

Section: Discussionmentioning

confidence: 99%

Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME)

et al. 2021

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Alterations in the carnitine shuttle system may be an indication of the presence of cancer. As such, in-depth analyses of this pathway in different malignant tumors could be important for the detection and treatment of this disease. The current study aims to assess the profiles of carnitine and acylcarnitines in gliomas with respect to their grade, the presence of isocitrate dehydrogenase (IDH) mutations, and 1p/19q co-deletion. Brain tumors obtained from 19 patients were sampled on-site using solid-phase microextraction (SPME) immediately following excision. Analytes were desorbed and then analyzed via liquid chromatography–high-resolution mass spectrometry. The results showed that SPME enabled the extraction of carnitine and 22 acylcarnitines. An analysis of the correlation factor revealed the presence of two separate clusters: short-chain and long-chain carnitine esters. Slightly higher carnitine and acylcarnitine concentrations were observed in the higher-malignancy tumor samples (high vs. low grade) and in those samples with worse projected clinical outcomes (without vs. with IDH mutation; without vs. with 1p/19q co-deletion). Thus, the proposed chemical biopsy approach offers a simple solution for on-site sampling that enables sample preservation, thus supporting comprehensive multi-method analyses.

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“…This approach allowed analyzing 96 samples within 45 min. Besides, a microfluidic open interface device enabled direct coupling of biocompatible SPME with MSeMS for determining tranexamic acid in plasma, with a sample throughput of 120 h À1 [45].…”

Section: Miniaturization and Automationmentioning

confidence: 99%

Greening sample preparation: An overview of cutting-edge contributions

Pena‐Pereira

Lavilla

Bendicho

2021

Current Opinion in Green and Sustainable Chemistry

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Solid phase microextraction coupled to mass spectrometryviaa microfluidic open interface for rapid therapeutic drug monitoring

Abstract: Tranexamic acid (TXA) is an antifibrinolytic used during cardiac surgery that presents high inter-patient variability.

Cited by 30 publications

References 31 publications

Metabolomic Phenotyping of Gliomas: What Can We Get with Simplified Protocol for Intact Tissue Analysis?

Metabolomic Phenotyping of Gliomas: What Can We Get with Simplified Protocol for Intact Tissue Analysis?

Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME)

Greening sample preparation: An overview of cutting-edge contributions

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