Solid-phase extraction technique for the analysis of biological samples

Krishnan, T. R.; Ibraham, I.

doi:10.1016/0731-7085(94)90001-9

Cited by 48 publications

(18 citation statements)

References 73 publications

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“…Because SPE is the number one sample preparation technique, it was reviewed regularly [Krishnan and Ibraham, 1994;Hennion, 1999;Majors, 2007]. These reviews provide a basic understanding of the SPE technology.…”

Section: Solid-phase Extraction (Spe)mentioning

confidence: 99%

Historical review of sample preparation for chromatographic bioanalysis: pros and cons

Chang

Zhang

et al. 2007

Drug Development Research

104

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Sample preparation is a major task in a regulated bioanalytical laboratory. The sample preparation procedure significantly impacts assay throughput, data quality, analysis cost, and employee satisfaction. Therefore, selecting and optimizing an appropriate sample preparation method is essential for successful method development. Because of our recent expertise, this article is focused on sample preparation for high-performance liquid chromatography with mass spectrometric detection. Liquid chromatography with mass spectrometric detection (LC-MS) is the most common detection technique for small molecules used in regulated bioanalytical laboratories. The sample preparation technologies discussed are pre-extraction and post-extraction sample processing, protein precipitation (PPT), liquid-liquid extraction (LLE), offline solid-phase extraction (SPE), and online solid-phase extraction. Since all these techniques were in use for more than two decades, numerous applications and variations exist for each technique. We will not attempt to categorize each variation. Rather, the development history, a brief theoretical background, and selected references are presented. The strengths and the limitations of each method are discussed, including the throughput improvement potential. If available, illustrations from presentations at various meetings by our laboratory are used to clarify our opinion. Drug Dev Res 68: 2007 r2007 Wiley-Liss, Inc.

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Section: Solid-phase Extraction (Spe)mentioning

confidence: 99%

Historical review of sample preparation for chromatographic bioanalysis: pros and cons

Chang

Zhang

et al. 2007

Drug Development Research

104

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“…Both Cloretazine TM and VNP4090CE had a noisy baseline with low recovery (the cps were 20-30% less than the neat samples at the same concentration). Since Cloretazine TM is a basic compound with pK a of approximately 8.0, it can be retained on SPE with a cation exchange stationary phase [15][16][17]. During development of a SPE extraction method for Cloretazine TM , VNP4090CE, and ISTD in plasma, we evaluated two different SPE extracting conditions on Phenomenex Strata-X-CW (weaker cation exchange).…”

Section: Plasma Sample Preparationmentioning

confidence: 99%

Determination of Cloretazine™ (VNP40101M) and its active metabolite (VNP4090CE) in human plasma by liquid chromatography electrospray tandem mass spectrometry (LC–ESI-MS/MS)

Bai

Minkin

Fraga

et al. 2007

Journal of Chromatography B

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“…They are likely to interfere with mass spectrometric detection due to ion suppression effects, and therefore have to be removed. Apart from protein precipitation [25], a well-established approach to this problem is the use of on-line solid phase extraction (SPE) [26]. In order to separate high-molecular biopolymers from low-molecular drug molecules, the so called restricted access materials (RAM) have been proven highly useful for the on-line clean-up of biofluids [27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

On-line solid-phase extraction high-performance liquid chromatography-tandem mass spectrometry for the quantitative analysis of tacrolimus in whole blood hemolyzate

et al. 2012

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Solid-phase extraction technique for the analysis of biological samples

Cited by 48 publications

References 73 publications

Historical review of sample preparation for chromatographic bioanalysis: pros and cons

Historical review of sample preparation for chromatographic bioanalysis: pros and cons

Determination of Cloretazine™ (VNP40101M) and its active metabolite (VNP4090CE) in human plasma by liquid chromatography electrospray tandem mass spectrometry (LC–ESI-MS/MS)

On-line solid-phase extraction high-performance liquid chromatography-tandem mass spectrometry for the quantitative analysis of tacrolimus in whole blood hemolyzate

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