Solid phase extraction of DNA from biological samples in a post-based, high surface area poly(methyl methacrylate) (PMMA) microdevice

Reedy, Carmen R.; Price, Carol W.; Sniegowski, J.J.; Ferrance, Jerome P.; Begley, Matthew R.; Landers, James P.

doi:10.1039/c0lc00597e

Cited by 63 publications

(50 citation statements)

References 29 publications

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“…Stainless steel is the most common material used in industrial processes (Zacheus et al, 2000). Polymethyl methacrylate (PMMA) is widely used in laboratory-scale biore-actors (Ferreira et al, 2010;Manuel et al, 2007;Matsuura et al, 2013;Reedy et al, 2011). To understand the microbial behaviour in the first steps of the biofilm formation process, the effects of the surface type and the exposure to BDMDAC was assessed on the bacterial physicochemical surface properties and on the initial adhesion to the selected surfaces.…”

Section: Introductionmentioning

confidence: 99%

The effects of surface type on the removal of Bacillus cereus and Pseudomonas fluorescens single and dual species biofilms

Lemos

Gomes

Mergulhão

et al. 2015

Food and Bioproducts Processing

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The aim of this work was to assess the effectiveness of the biocide benzyldimethyldodecyl ammonium chloride (BDMDAC) on the removal of single and dual species biofilms of Bacillus cereus and Pseudomonas fluorescens formed in a rotating cylinder reactor (RCR), using AISI316 stainless steel (SS) and polymethyl methacrylate (PMMA) as adhesion surfaces. Additional tests were performed to understand the adhesion of B. cereus and P. fluorescens to the selected surfaces.Predictions of the adhesion potential according to the thermodynamic theory showed more favourable adhesion on SS than on PMMA, for both species.Thermodynamically, adhesion was more favourable for B. cereus. After BDMDAC treatment, thermodynamic adhesion ability was favoured for P. flurescens and decreased for B. cereus, mainly on PMMA. Both bacteria had negative surface charge and the exposure to BDMDAC increased the charge to less negative values. In vitro adhesion results were, for most cases, contradictory to those predicted by the thermodynamic theory. Single and dual species biofilms were formed in the RCR for 7 days. Afterwards, the biofilms were exposed to the chemical (use of BDMDAC) and to hydrodynamic stresses (use of increasing Reynolds number of agitation), alone and combined. The applications of BDMDAC or hydrodynamic stress, when applied alone, were insufficient to remove the biofilms from the surfaces. The combined effects of BDMDAC with a series of increasing Reynolds number of agitation promoted additional biofilm removal. This effect was dependent on the surface used. For PMMA, the hydrodynamic stress was more effective on the removal of BDMDAC-treated dual species biofilms. For SS, the synergy of the chemical and hydrodynamic stresses removed more B. cereus and dual species biofilms. The overall results 2 demonstrate that the species association was not advantageous in biofilm resistance to removal when compared with the single species biofilms, particularly those of P. fluorescens. In general, removal by hydrodynamic stress, alone and preceded by the BDMDAC treatment, was higher for biofilms formed on SS. However, even the combined action of BDMDAC and the exposure to a series of increasing Reynolds number of agitation were not effective to obtain biofilm-free surfaces.

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Section: Introductionmentioning

confidence: 99%

The effects of surface type on the removal of Bacillus cereus and Pseudomonas fluorescens single and dual species biofilms

Lemos

Gomes

Mergulhão

et al. 2015

Food and Bioproducts Processing

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“…20 µl of a mixture of 33% 1,2-dichloro ethane in 67% v/v ethanol in between the layers. 21,22 The chips were then clamped between microscope slides using bulldog clips, and were left for 24h at 65 • C. The fluidic channels were cautiously flushed with DI water prior to heat treatment, which prevented them from clogging. Bonded chips were flushed with 200 µl 100% ethanol (to minimise the formation of bubbles in the sample chamber) followed by 400 µl DI water prior to filling with 200 µl tap water or europium chloride solution for NMR.…”

Section: Microfluidic Chipsmentioning

confidence: 99%

Structural shimming for high-resolution nuclear magnetic resonance spectroscopy in lab-on-a-chip devices

2014

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High-resolution proton NMR spectroscopy is well-established as a tool for metabolomic analysis of biological fluids at the macro scale. Its full potential has, however, not been realised yet in the context of microfluidic devices. While microfabricated NMR detectors offer substantial gains in sensitivity, limited spectral resolution resulting from mismatches in the magnetic susceptibility of the sample fluid and the chip material remains a major hurdle. In this contribution, we show that susceptibility broadening can be compensated even in the presence of substantial mismatch by including suitably shaped compensation structures into the chip design. An efficient algorithm for the calculation of field maps from arbitrary chip layouts based on Gaussian quadrature is used to optimise the shape of the compensation structure to ensure a flat field distribution inside the sample area. Previously, the complexity of microfluidic NMR systems has been restricted to simple capillaries to avoid susceptibility broadening. The structural shimming approach introduced here can be adapted to virtually any shape of sample chamber and surrounding fluidic network, thereby greatly expanding the design space and enabling true lab-on-a-chip systems suitable for high-resolution NMR detection.

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“…Recently, the use of polymethyl methacrylate (PMMA) has become a prevalent substrate for use in microfluidics (Nugen et al 2009;Reedy et al 2011;Wang et al 2003), due to its low cost, chemical inertness and manufacturability.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of a capillary-flow microfluidic device for nucleic acid detection

Jin

Dai

et al. 2012

Microsyst Technol

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We have developed a capillary flow-driven microfluidic biosensor to meet the needs of diagnostics for resource-limited areas. The device combined elements of lateral flow assays and microfluidic technology resulting in a hybrid with benefits of both formats. The biosensor was achieved by bonding two pieces of polymethyl methacrylate with channels ablated by a CO 2 laser, and enclosing an absorbent pad. The channels were UV/ozone treated to increase hydrophilicity which enabled capillary flow. The absorbent pad allowed for continuous flow in the channels once filled. The application of biosensor was demonstrated by detection of DNA with a sandwich assay. The target DNA was hybridized with nucleic acid modified magnetic beads as well as Ru(bpy) 3 2? doped silica nanoparticles. Fluorescent signals were quantified in a holder fabricated to fit in a fluorescent microtiter plate reader. The capillary flow microfluidic was capable to detect 1 pmol target. The assay format which features rapid analysis and does not require the use of pumps could allow for inexpensive point of care diagnostics in the future.

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Solid phase extraction of DNA from biological samples in a post-based, high surface area poly(methyl methacrylate) (PMMA) microdevice

Cited by 63 publications

References 29 publications

The effects of surface type on the removal of Bacillus cereus and Pseudomonas fluorescens single and dual species biofilms

The effects of surface type on the removal of Bacillus cereus and Pseudomonas fluorescens single and dual species biofilms

Structural shimming for high-resolution nuclear magnetic resonance spectroscopy in lab-on-a-chip devices

Development and characterization of a capillary-flow microfluidic device for nucleic acid detection

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