Solid Phase Extraction of Cd, Cu, and Ni from Leafy Vegetables and Plant Leaves Using Amberlite XAD-2 Functionalized with 2-Hydroxy-acetophenone-thiosemicarbazone (HAPTSC) and Determination by Inductively Coupled Plasma Atomic Emission Spectroscopy

Rao, G. Purna Chandra; Seshaiah, K.; Rao, Yerra Koteswara; Wang, M.C.

doi:10.1021/jf0600049

Cited by 72 publications

(19 citation statements)

References 20 publications

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“…Accurate analytical methods have been developed to detect metal ions in different matrixes or samples [2][3][4], but they require complex equipments and sophisticated lab manipulations. So, the development of low-cost, user-friendly, quantitative and qualitative detection systems for metal revealing is of paramount importance.…”

Section: Introductionmentioning

confidence: 99%

Development of a Biosensor for Copper Detection in Aqueous Solutions Using an Anemonia sulcata Recombinant GFP

Masullo

Puccio²,

Pierro³

et al. 2013

Appl Biochem Biotechnol

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Fluorescent proteins from marine organisms represent potential candidates for biosensor development. In this paper, we described the isolation of a native green fluorescent protein from Anemonia sulcata and the cloning and purification of its equivalent as a recombinant protein in Escherichia coli. Furthermore, the spectroscopic behaviours of the native and recombinant GFPs were investigated as a function of Cu concentration. Our results suggest the high selectivity of both proteins at copper than the other metals and, for the recombinant protein, a great sensitivity at a very low concentration (0.1-1 μM). Moreover, starting from these data, using the combination of molecular biology Appl Biochem Biotechnol (2014) techniques and optical setup, we developed a device for the detection of Cu 2+ in water solutions. The quenching effect detected with the device showed that the relative attenuation of the signal (0.46±0.02 AU) was slightly larger than the data measured by fluorescence spectra (0.65±0.03 AU). The good sensitivity in the span of two orders of the magnitude of Cu 2+ concentration, the fact that the instrument is made up of low-cost and sturdy parts and the selective quenching of rAsGFP to copper ions make this setup suited as a low cost, on-thefield, copper ion-specific biosensor.

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Section: Introductionmentioning

confidence: 99%

Development of a Biosensor for Copper Detection in Aqueous Solutions Using an Anemonia sulcata Recombinant GFP

Masullo

Puccio²,

Pierro³

et al. 2013

Appl Biochem Biotechnol

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“…They included atomic absorption spectrometry [4,5], fast neutron activation analysis [6], inductively coupled plasma atomic emission spectrometry [7], and electrochemical methods [8][9][10]. Among these methods, some of them needed relatively high cost apparatus and were not easily adaptable for online monitoring.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive fluorescent sensor for copper (II) based on amplified fluorescence quenching of a water-soluble NIR emitting conjugated polymer

et al. 2012

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We have synthesized the near-infrared watersoluble conjugated polymer poly[2,5-di(propyloxysulfonate)-1,4-phenylene-ethynylene-9,10-anthrylene (referred to as PPEASO3). Its fluorescence (at wavelengths between 650 and 800 nm following photoexcitation at 550 nm) is efficiently quenched by Cu(II) ions, while other physiologically relevant metal ions do not cause significant quenching at the same concentrations. Under optimum conditions, fluorescence intensity is inversely proportional to the concentration of Cu (II). The calibration curve displays two linear regions over the range of 0-3.2×10 −7 mol L −1 and 3.2×10 −7 mol L −1 to 1.0× 10 −4 mol L −1 of Cu(II), respectively. The long-wavelength excitation and emission can substantially reduce interferences by the autofluorescence and light scattering of biological matter under UV excitation. The method was successfully applied to the determination of Cu(II) in synthetic and tea samples.

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“…[9][10][11][12][13][14] However, most of these methods still suffer from shortcomings including complex operation process, expensive instruments, or time-consuming pretreatment, which limit their uses for analysis of Cu 2+ or hemin. Fluorescence spectroscopy is one of the most common techniques used for routine analysis.…”

Section: 4mentioning

confidence: 99%

Cytidine-stabilized copper nanoclusters as a fluorescent probe for sensing of copper ions and hemin

et al. 2018

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We reported a sensitive and selective fluorescence "turn on-off" strategy for detection of Cu 2+ and hemin, respectively. The fluorescence "turn on" sensor for Cu 2+ detection had a wide linear range of 0.05-2.0 mM with a limit of detection (LOD) of 0.032 mM, and the fluorescence "turn off" sensor for hemin detection possessed a wide linear range of 0.05-4.0 mM with an LOD of 0.045 mM. The sensor for Cu 2+ or hemin exhibited high selectivity over other possible substances. In addition, it was demonstrated by using various analytical characterization techniques that the fluorescence "turn on" sensor for Cu 2+ was constructed on the basis of the formation of water-soluble fluorescent copper nanoclusters (CuNCs), and the fabrication of the fluorescence "turn off" sensor for hemin was predominately based on the inner filter effect of hemin on the fluorescence of the CuNCs. Finally, the proposed fluorescence "turn on-off" sensor system was successfully applied for detection of Cu 2+ in lake water samples and hemin in duck blood samples.

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Solid Phase Extraction of Cd, Cu, and Ni from Leafy Vegetables and Plant Leaves Using Amberlite XAD-2 Functionalized with 2-Hydroxy-acetophenone-thiosemicarbazone (HAPTSC) and Determination by Inductively Coupled Plasma Atomic Emission Spectroscopy

Cited by 72 publications

References 20 publications

Development of a Biosensor for Copper Detection in Aqueous Solutions Using an Anemonia sulcata Recombinant GFP

Development of a Biosensor for Copper Detection in Aqueous Solutions Using an Anemonia sulcata Recombinant GFP

Highly sensitive fluorescent sensor for copper (II) based on amplified fluorescence quenching of a water-soluble NIR emitting conjugated polymer

Cytidine-stabilized copper nanoclusters as a fluorescent probe for sensing of copper ions and hemin

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