Solid phase extraction—Non-aqueous capillary electrophoresis for determination of metformin, phenformin and glyburide in human plasma

Lai, Edward P. C.; Feng, S.

doi:10.1016/j.jchromb.2006.05.030

Cited by 57 publications

(37 citation statements)

References 21 publications

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“…There are numerous methods that can measure metformin within biological fluids. These include capillary electrophoresis [10,11] and high performance liquid chromatography (HPLC) [12][13][14]. The latter being the most widely applied technique and is routinely linked to tandem mass spectrometry (MS/MS).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Michel

Gaunt

Arnason

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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A simple, fast and sensitive quantification method for the drug metformin in dog serum was developed using flow injection analysis (FIA)-tandem mass spectrometry (MS/MS). The method was fully validated according to industry standards. It is the first time that FIA-MS/MS for metformin was developed surpassing all existing methods in terms of time of analysis. The quantification method was dependent on the formation of [M+H] + using electrospray ionization (ESI) and employing multiple reaction monitoring (MRM) using quadrupole-linear ion trap (4000 QTRAP ® ) instrument. A deuterated internal standard (IS) of metformin bearing six deuterium atoms was used to compensate for matrix effects and for variation in ion current within the ESI source. The ion transitions that were monitored were m/z 130.1 m/z 71.0 and m/z 130.1 m/z 60.1 for metformin and m/z 136.0 m/z 77.0 for the internal standard. A linear response (r = 0.9966) was established for a range of concentrations of 5-2340 ng/ml. The inter-and intra-day variations were within the acceptable criteria for all quality control samples. The method was successfully applied for measurement of serum metformin concentration in dogs after intravenous injection.

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Section: Introductionmentioning

confidence: 99%

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Michel

Gaunt

Arnason

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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“…Few CE methods have been reported for the determination of metformin or with other anti-diabetic agents in plasma [27,28]. There are also involved sample pretreatment procedures prior to analysis for plasma.…”

Section: Introductionmentioning

confidence: 99%

CE with direct sample injection for the determination of metformin in plasma for type 2 diabetic mellitus: An adequate alternative to HPLC

Wei

Yeh

Liao

et al. 2009

J of Separation Science

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We developed a simple and selective CE with UV detection at 233 nm for the analysis of metformin in plasma based on direct sample injection without any pretreatment. The sample was employed with an electrokinetic injection of 10 kV for 100 s. CE separation of metformin from biological matrix was performed at 25 degrees C using a BGE consisting of 25 mM Tris buffer at pH 4.0. The linear range of the CE method for the determination of metformin in plasma was over the range of 0.1-2.0 mug/mL; the LOD of the drug in plasma (S/N = 3; injection 10 kV, 100 s) was 30 ng/mL. Data by CE were compared with the results obtained by a validated HPLC method. CE assay of metformin exhibited a very good correlation (r(2 )= 1) with respect to HPLC. CE determination of metformin is a robust, sensitive, and reproducible method with the advantage over HPLC of being fast, without prior extraction, or precipitation of proteins, also enabling quick assessment of metformin for pharmacokinetic and clinical studies.

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“…Various methods for determination and quantitation of MET in biological samples have been published [5][6][7][8][9][10][11][12][13][14][15][16]. However, most of them have a complex sample preparation.…”

Section: Introductionmentioning

confidence: 99%

“…The usual methods are precipitation [5][6][7][8][9][10][11], liquid-liquid extraction [12] or solid phase extraction [13][14][15]. Therefore, the actual trends have an approach that follows these kinds of procedure.…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid method determination for metformin in human plasma using high performance liquid chromatography tandem mass spectrometry: Application to pharmacokinetic studies

Marques

Soares

Pinto

et al. 2007

Journal of Chromatography B

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Solid phase extraction—Non-aqueous capillary electrophoresis for determination of metformin, phenformin and glyburide in human plasma

Cited by 57 publications

References 21 publications

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

CE with direct sample injection for the determination of metformin in plasma for type 2 diabetic mellitus: An adequate alternative to HPLC

Simple and rapid method determination for metformin in human plasma using high performance liquid chromatography tandem mass spectrometry: Application to pharmacokinetic studies

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