Solid-phase extraction in bioanalytical applications

Sentellas, Sònia; Saurina, Javier; Núñez, Óscar

doi:10.1016/b978-0-12-816906-3.00025-x

Cited by 5 publications

(5 citation statements)

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“…Solid‐phase extraction (SPE) is a common sample preparation technique for the determination of analytes from biomatrices by HPLC (Sentellas, Saurina, & Núñez, 2020). It is mainly used for the isolation and concentration of organic species from complex samples, but SPE can be combined with derivatization leading to a specific derivatization, known as solid‐phase analytical derivatization (SPAD) (Atapattu & Rosenfeld, 2018).…”

Section: Derivatization and Solid‐phase Extractionmentioning

confidence: 99%

“…Some of the recent applications and the performances of derivatization in bioanalysis based on LLE procedure are included in Table 1. S C H E M E 4 The principle of derivatization combines with liquidliquid extraction6 | DERIVATIZATION AND SOLID-PHASE EXTRACTIONSolid-phase extraction (SPE) is a common sample preparation technique for the determination of analytes from biomatrices by HPLC(Sentellas, Saurina, & Núñez, 2020). It is mainly used for the isolation and concentration of organic species from complex samples, but SPE can be combined with derivatization leading to a specific derivatization, known as solid-phase analytical derivatization (SPAD)(Atapattu & Rosenfeld, 2018).…”

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confidence: 99%

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Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

David

Moldoveanu

Galaon

2020

Biomedical Chromatography

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Derivatization, or chemical structure modification, is often used in bioanalysis performed by liquid chromatography technique in order to enhance detectability or to improve the chromatographic performance for the target analytes. The derivatization process is discussed according to the analytical procedure used to achieve the reaction between the reagent and the target compounds (containing hydroxyl, thiol, amino, carbonyl and carboxyl as the main functional groups involved in derivatization). Important procedures for derivatization used in bioanalysis are in situ or based on extraction processes (liquid-liquid, solid-phase and related techniques) applied to the biomatrix. In the review, chiral, isotope-labeling, hydrophobicity-tailored and post-column derivatizations are also included, based on representative publications in the literature during the last two decades. Examples of derivatization reagents and brief reaction conditions are included, together with some bioanalytical applications and performances (chromatographic conditions, detection limit, stability and sample biomatrix).

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Section: Derivatization and Solid‐phase Extractionmentioning

confidence: 99%

mentioning

confidence: 99%

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

David

Moldoveanu

Galaon

2020

Biomedical Chromatography

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“…Solid-phase extraction (SPE) is a popular option for the preparation of various types of samples, including human plasma-the most commonly used sample in TDM. Its selectivity, purification efficacy, versatility, and ease of automation are among the most prominent advantages that make it suitable for complex bioanalytical sample preparation [6].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of Solid-Phase Extraction and LC-MS/MS Analysis of Six Breast Cancer Drugs in Patient Plasma Samples

Turković,

Mutavdžić Pavlović,

Mlinarić

et al. 2023

Pharmaceuticals

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In the development of bioanalytical LC-MS methods for the determination of drugs in plasma samples in a clinical setting, adequate sample preparation is of utmost importance. The main goals are to achieve the selective extraction of the analytes of interest and attain thorough matrix removal while retaining acceptable ecological properties, cost-effectiveness, and high throughput. Solid-phase extraction (SPE) offers a versatile range of options, from the selection of an appropriate sorbent to the optimisation of the washing and elution conditions. In this work, the first SPE method for the simultaneous extraction of six anticancer drugs used in novel therapeutic combinations for advanced breast cancer treatment—palbociclib, ribociclib, abemaciclib, anastrozole, letrozole, and fulvestrant—was developed. The following sorbent chemistries were tested: octylsilyl (C8), octadecylsilyl (C18), hydrophilic–lipophilic balance (HLB), mixed-mode cation-exchange (MCX and X-C), and mixed-mode weak cation-exchange (WCX), with different corresponding elution solvents. The samples were analysed using LC-MS/MS, with a phenyl column (150 × 4.6 mm, 2.5 μm). The best extraction recoveries (≥92.3%) of all analytes were obtained with the C8 phase, using methanol as the elution solvent. The optimised method was validated in the clinically relevant ranges, showing adequate precision (inter-day RSD ≤ 14.3%) and accuracy (inter-day bias −12.7–13.5%). Finally, its applicability was successfully proven by the analysis of samples from breast cancer patients.

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“…This separation method is one of the most important for extracting species from liquid media because the retained specie is concentrated in a solid phase. Afterward, a volume of an extract is passed by the solid phase, removing the retained specie to a volume that is considerably lower when compared to the initial volume [ 18 , 19 ]. Thus, several adsorbents have been utilized for the uptake of REEs, such as zeolites [ 20 ], amino-functionalized silica materials [ 21 ], composite materials of CaCO 3 :Eu 3+ @SiO 2 [ 22 ], organofunctionalized minerals [ 23 ], polymers [ 24 ], algae [ 25 ], fungus [ 26 ], and others.…”

Section: Introductionmentioning

confidence: 99%

Biosorption of Neodymium (Nd) from Aqueous Solutions Using Spirulina platensis sp. Strains

et al. 2022

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Rare earth elements such as neodymium (Nd) are important elements used mainly in developing new technologies. Although they are found in low concentrations in nature, they can be obtained by extracting solid samples such as phosphogypsum. Among the techniques, adsorption has been used successfully with several adsorbent materials. In this work, two strains of Spirulina platensis (LEB-18 and LEB-52) were employed as biosorbents for efficiently removing the Nd element from the aqueous media. Biosorption tests were carried out in a batch system, and the results of the biosorption kinetics showed that for both materials, the biosorption of Nd was better described by the Avrami model. Moreover, it could be considered that 80 min would be necessary to attain the equilibrium of Nd(III) using both biosorbents. The result of the biosorption isotherms showed that for both strains, the best-fitted model was the Liu model, having a maximum biosorption capacity of 72.5 mg g−1 for LEB-18 and 48.2 mg g−1 for LEB-52 at a temperature of 298 K. Thermodynamics of adsorption showed that for both LEB-18 and LEB-52 the process was favorable (∆G° < 0) and exothermic (∆H° −23.2 for LEB-18 and ∆H° −19.9 for LEB-52). Finally, both strains were suitable to uptake Nd, and the better result of LEB-18 could be attributed to the high amount of P and S groups in this biomass. Based on the results, a mechanism of electrostatic attraction of Nd3+ and phosphate and sulfate groups of both strains of Spirulina platensis was proposed.

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Solid-phase extraction in bioanalytical applications

Cited by 5 publications

References 45 publications

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

Optimisation of Solid-Phase Extraction and LC-MS/MS Analysis of Six Breast Cancer Drugs in Patient Plasma Samples

Biosorption of Neodymium (Nd) from Aqueous Solutions Using Spirulina platensis sp. Strains

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