Solid-phase extraction coupling to capillary electrophoresis with emphasis on environmental analysis

Martínez, Diego Luis Valera; Cugat, M. J.; Borrull, Francesc; Calull, Marta

doi:10.1016/s0021-9673(00)00839-6

Cited by 93 publications

(45 citation statements)

References 133 publications

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“…It is intresting to note that among few sorts of sample preparation SPE has achieved a good reputation in analytical science due to its ease of operation, selective, time and costly chemical saving nature [8]. Some reviews have been published on the pre-treatment and sample preparation methodologies for the achiral analysis of pollutants [9][10][11][12][13]. Dabek-Zlotorzynska et al [10] reviewed the sample pretreatment methodologies for environmental analysis prior to CE.…”

Section: Sample Treatmentmentioning

confidence: 99%

Chiral Analyses of Pollutants by Capillary Electrophoresis

Ali¹

2010

TOCBMJ

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Abstract:The determination of enantiomeric composition of chiral xenobiotics and pollutants is a very difficult job due to their low amounts and poor detection by commonly used UV detector. But this sort of analysis is an important issue from the health point of view. The chiral analyses of these notorious pollutants by capillary electrophoresis have been discussed in this article. This review discusses the new trends and advancements, which have been achieved in the analyses of chiral pollutants using capillary electrophoresis and the state-of-art of their enaniomeric resolution. This article focuses on sample treatment, applications, optimization, detection, mechanisms of chiral resolution and future perspectives of CE in chiral resolution of xenobiotics. Besides, efforts have also been made to suggest the improvement in CE machine to make it ideal for the analyses of chiral xenobiotics at trace levels.

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Section: Sample Treatmentmentioning

confidence: 99%

Chiral Analyses of Pollutants by Capillary Electrophoresis

Ali¹

2010

TOCBMJ

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“…Because of their extremely low concentration, a preliminary concentration step i.e., preconcentration is usually necessary prior to the determination of the metals (Yaman 1999;Anezaki et al 1998;Zolotov et al 1995;Karadjova 1999;Shar and Soomaro 2004;Qufen et al 2002). Nowadays, many separation/preconcentration techniques for metal ions determination include liquid-liquid extraction, (Liska 1993;Martinez et al 2000;Bruzzoniti et al 2000) ion-exchange resins, (Cao and Fang 1998;Yang et al 2004) resin chelation and solid-phase extraction (Alonso et al 2001;Henglein 1989;Xue and Xu 2003). Of all theses methods, solid phase extraction has been widely used in comparison with traditional extraction techniques; since it is simple, rapid and inexpensive, less polluting to the environment and can be easily automated.…”

Section: Introductionmentioning

confidence: 99%

Applications of organo-silica nanocomposites for SPNE of Hg(II)

Kaur

2015

Appl Nanosci

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An analytical method using modified SiO 2 nanoparticles as solid-phase extractant has been developed for the preconcentration of trace amounts of Hg(II) in different water samples. Conditions of the analysis such as preconcentration factor, effect of pH, sample volume, shaking time, elution conditions and effects of interfering ions for the recovery of analyte were investigated. The adsorption capacity of nanometer SiO 2 -APTMS was found to be 181.42 lmol g -1 at optimum pH and the detection limit (3r) was 0.45 lg L -1 . The extractant showed rapid kinetic sorption. The adsorption equilibrium of Hg(II) on nanometer SiO 2 -APTMS was achieved just in 15 min. Adsorbed Hg(II) was easily eluted with 4 mL of 2.0 M hydrochloric acid. The maximum preconcentration factor was 75. The method was applied for the determination of trace amounts of Hg(II) in various synthetic samples and water samples.

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“…Generally, it is applied to the 30 selective capture and preconcentration of the target analyte and/or the removal of potentially interfering components from the sample matrix. [1][2][3][4] SPE has been applied as a sample preparation strategy both for small molecules (pharmaceutical, pesticides etc) as well as larger biomolecules (peptides, proteins etc). SPE 35 devices usually comprise a selective adsorbant material encased within a housing such that the sample and subsequent elution buffers can be passed through the bed for collection and analysis.…”

Section: Introductionmentioning

confidence: 99%

Pipette-tip selective extraction of glycoproteins with lectin modified gold nano-particles on a polymer monolithic phase

Alwael

Connolly

Clarke

et al. 2011

Analyst

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The selective extraction of specific proteins (non-glycosylated, glycosylated or different glycoforms) from complex sample matrices utilising selective solid phase extration (SPE) is of significant interest within the fields of proteomics and glycoproteomics. Polymer monoliths have proven to be an excellent solid support for SPE applications in bio-analysis due to their excellent mass transfer characteristics for large biomolecules. Although biorecognition molecules such as lectins can be covalently immobilised directly onto the monolith surface using a variety of chemistries, the relatively low surface area of these monoliths results in a correspondingly low sample 10 capacity. Recently, we have described the covalent attachment of 20 nm gold nanoparticles (AuNPs) upon a polymer monolith with excellent surface coverage leading to a significant increase in surface area. In this work therefore we describe the in-situ preparation of an ethylene dimethacrylate porous polymer monolith within the confines of 20 µL polypropylene pipette tips, onto which methacrylate anchor sites were previously grafted to ensure intimate monolith/wall contact. Then AuNPs were immobilised onto the monolith pore surface utilising azlactone cemistry. Field emission scanning electron microscopy was used to verify the high surface coverage of 15 AuNPs. Erythrina cristagalli lectin (ECL) was immobilised upon the attached AuNPs via a biofunctional linker. The ECL-modified tip was successfully applied for the enrichment of galactosylated protein (desialated transferrin) versus a non-galactosylated protein (ribonuclease B) due to the specificity of ECL. Reversed-phase capillary HPLC was used to validate the efficiency and selectivity of the developed micro-extraction phase which resulted in an increase in extraction recovery of ~95 % in the presence of AuNPs. The specificity of the ECL-modified tip was further studied by using more complex mixture of proteins which included non-glycosylated 20 proteins and glycosylated proteins with different terminal sugar structure. Finally, the lectin affinity extraction device was tested with a real sample (E. coli cell lysate) spiked with target galactosylated glycoproteins.

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Solid-phase extraction coupling to capillary electrophoresis with emphasis on environmental analysis

Cited by 93 publications

References 133 publications

Chiral Analyses of Pollutants by Capillary Electrophoresis

Chiral Analyses of Pollutants by Capillary Electrophoresis

Applications of organo-silica nanocomposites for SPNE of Hg(II)

Pipette-tip selective extraction of glycoproteins with lectin modified gold nano-particles on a polymer monolithic phase

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