Solid-Phase Extraction Coupled with HPLC-DAD for Determination of B Vitamin Concentrations in Halophytes

Chamkouri, Narges; Khodadoust, Saeid; Ghalavandi, Forogh

doi:10.1093/chromsci/bmv080

Cited by 8 publications

(9 citation statements)

References 18 publications

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“…Castañeda-Loaiza et al [32] and Lima et al [5] use the extraction and analytical methods proposed by Santos et al [88] for fat-soluble and watersoluble vitamins. Various HPLC methods involving a photodiode array detector (PDA) or fluorescence detector (FL) are widely used; only Chamkouri et al [89] use a DAD and Zaier et al [90] an ultraviolet (UV) detector in their analytical methods.…”

Section: Vitaminsmentioning

confidence: 99%

“…Compounds considered as B complex vitamins are antioxidants and essential coenzymes, supporting vital biological processes [80,89]. For example, thiamine (B1) is essential to carbohydrate metabolism, pyridoxine (B6) to protein metabolism, folate (B9) to the synthesis of nucleic acids, and cobalamin (B12) for the nervous system, cell growth, and bone health [5,89].…”

Section: Vitaminsmentioning

confidence: 99%

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Extraction and Quantification of Chlorophylls, Carotenoids, Phenolic Compounds, and Vitamins from Halophyte Biomasses

2022

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Halophytes are salt-tolerant plants, and they have been utilised as healthy, nutritious vegetables and medicinal herbs. Various studies have shown halophytes to be rich in health-beneficial compounds with antioxidant activity, anti-inflammatory and antimicrobial effects, and cytotoxic properties. Despite their potential, these plants are still underutilised in agriculture and industrial applications. This review includes the state-of-the-art literature concerning the contents of proanthocyanidins (also known as condensed tannins), total phenolic compounds, photosynthetic pigments (chlorophyll and carotenoids), and vitamins in various halophyte biomasses. Various extraction and analytical methods are also considered. The study shows that various species have exhibited potential for use not only as novel food products but also in the production of nutraceuticals and as ingredients for cosmetics and pharmaceuticals.

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Section: Vitaminsmentioning

confidence: 99%

Section: Vitaminsmentioning

confidence: 99%

Extraction and Quantification of Chlorophylls, Carotenoids, Phenolic Compounds, and Vitamins from Halophyte Biomasses

2022

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“…The instrument was operated with frequency range of 500-4000 cm -1 . The resulted peaks in the FTIR spectrum were used to derive a dose-response curve and provide pharmacological information [33].…”

Section: Fourier Transformation Infrared Spectrometry (Ftir) Analysismentioning

confidence: 99%

Chitosan Nanogel Design on Gymnema sylvestre Essential Oils to Inhibit Growth of Candida albicans Biofilm and Investigation of Gene Expression ALS1, ALS3

Akbari

Bayat

Roudbarmohammadi

et al. 2019

Period. Polytech. Chem. Eng.

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Chitosan (CS) is a polycation with a pka of about 6.3 with a charge density dependent on pH and the %DA-value which can interact with polyanions to form complex and gels. Nanoparticles (CNPs) in addition can increase the antifungal potential of bioactive compounds like essential oils by increasing cellular interactions between them and the fungal as a result of the very small size that enhances cellular uptake. In this study, was set to investigate the encapsulation of the Gymnema sylvestre essential oils (G.EOs) using Chitosan and Myrestic acid made Nanogel in order to enhance its antifungal activity and stability to the oil against C. albicans strain (ATCC 10231). To procedure this, the self-assembled process of Chitosan and Myrestic acid Nanogel (CS-MA) through the 1- ethyl - 3- (3 dimethyl aminoprophyl) carbodiimide (EDC) was designed. Its physicochemical properties were determined by Fourier Transforms Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and microscopic methods by Atomic force microscopy (AFM), Transmission Electron Microscopy (TEM), and Scanning Electron Microscopy (SEM). Minimum inhibitory concentration (MIC) at 18.7 to 37.5 µg/ml and 2.3 to 4.6 µg/ml and minimal fungicidal concentration (MFC) at 75 µg/ml and 5.38 µg/ml using by broth micro dilution (BMD) method for G. sylvestre oils (G.EOs) and oil-loaded Nanogels (G.OLNPs) were measurement. The susceptibility of C. albicans biofilm to fractions was examined by 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[8phenylamino) carbonyl] - 2H - tetrazolium hydroxide (XTT) quantitatively.The concentration of G.OLNPs required to inhibit 50 % biofilm formation was 4.68 µg/ml, while that to remove 90 % biofilm growth was 18.07 µg/ml. In addition, it was observed that cell uptake of G.OLNPs was much higher compared with free G.EOs. Reverse transcription polymerase chain reaction (RT-PCR) analysis was performed to determine the effect of sub-MIC concentrations of G.EOs and G.OLNPs on expression of the biofilm–related gene ALS1 / ALS3, and indicated the G.OLNPs down-regulated the expression of hypha-specific gene ALS3. Furthermore, the data strongly suggested that G.OLNPs more effective suppressed C. albicans planktonic cells and reduction biofilm biomass.

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“…These secondary metabolites apparently act as a defense mechanism and are produced in human and animal food when suitable conditions exist. Obtaining of polyacetyl-derived mycotoxins is the most important pathway for mycotoxin biosynthesis due to the involvement of acetyl coenzyme A, which produces mycotoxins such as aflatoxin, ochratoxin, patulin, citrinin, and pentanedioic acid [6,7].…”

Section: Introductionmentioning

confidence: 99%

The eliminatory effects of cold argon plasma jet on aflatoxin B1 produced by different isolates of Aspergillus section nigri

Hassanpour

Bayat

Nosrati

et al. 2019

BCC

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Aflatoxin and ochratoxin are among the most resistant and severe toxins produced by Aspergillus spp. The aim of this study was an evaluation of eliminatory effects of cold argon plasma jet on aflatoxin B1. Sampling of wheat, corn, oatmeal, flour and rice products from northern cities of Iran was carried out. The fungal species were cultured on CHAPK medium and next to sabouraud dextrose broth + malt extract (SB + ME) and also SB + yeast extract (SB + YE) media to obtain aflatoxin B1. The ELISA test was conducted to measure the aflatoxin level. The mean initial concentration of the aflatoxin from charts analysis in the SB +ME medium was 16.106 μg / kg (10.4, 8.32 and 7.55 μg / kg at 30, 60 and 360 sec, respectively), and in the SB +YE medium from 23.699 μg / kg (12.82, 9.93 and 9.54 μg / kg, respectively), at the same time. Furthermore, the mean concentrations of aflatoxin B1 by tables analysis in the SB+ ME medium changed from 20.02 μg / kg to 6.87, 10.46 and 7.55 μg / kg, at 30, 60 and 360 sec, respectively, and in the medium SB +YE from 28.85 μg / kg reached 6.56.6, 8.43 and 7.02 μg / kg, respectively, at the same time. In this study, the change or decrease in the concentration of aflatoxin B1, from Lin/Log and Log/Lin analyses of charts was significant (p<0.05) at 60 s and 360 s of application of the cold argon plasma jet in both media. The cold plasma as a new technology in various fields of food and agriculture can provide effective and suitable solutions in order to promote the goals of food industry, especially in the areas of mycotoxin elimination.

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Solid-Phase Extraction Coupled with HPLC-DAD for Determination of B Vitamin Concentrations in Halophytes

Cited by 8 publications

References 18 publications

Extraction and Quantification of Chlorophylls, Carotenoids, Phenolic Compounds, and Vitamins from Halophyte Biomasses

Extraction and Quantification of Chlorophylls, Carotenoids, Phenolic Compounds, and Vitamins from Halophyte Biomasses

Chitosan Nanogel Design on Gymnema sylvestre Essential Oils to Inhibit Growth of Candida albicans Biofilm and Investigation of Gene Expression ALS1, ALS3

The eliminatory effects of cold argon plasma jet on aflatoxin B1 produced by different isolates of Aspergillus section nigri

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