Solid-Phase Extraction and High-Performance Liquid Chromatography for Simultaneous Determination of Important Bioactive Ginsenosides in Pharmaceutical Preparations

Lou, Dawei; Saito, Yoshihiro; Jinno, Kiyokatsu

doi:10.1365/s10337-005-0640-6

Cited by 21 publications

(19 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The solid-phase extraction sample (2 mL) was assayed according to the method described by Lou et al 29 using a C18 ODS cartridge (Waters Associates, Milford, MA, USA). The levels of 16 major ginsenosides were analyzed using a high-performance liquid chromatography (HPLC)-based technique developed by Lee et al 22 with a Varian Prostar 200 HPLC system (Varian, Inc., Palo Alto, CA, USA) equipped with a quaternary solvent delivery system, an autosampler, and UV detector, with measurements at 203 nm.…”

Section: High-performance Liquid Chromatography Analysis Of Ginsenosidesmentioning

confidence: 99%

Fermenting Red Ginseng Enhances Its Safety and Efficacy as a Novel Skin Care Anti-Aging Ingredient: In Vitro and Animal Study

Lee

Kim

Park

et al. 2012

Journal of Medicinal Food

View full text Add to dashboard Cite

The objective of this study was to evaluate the anti-aging potential and skin safety of red ginseng (RG) and fermented red ginseng (FRG) using Lactobacillus brevis for use as cosmetic ingredients. Concentrations of uronic acid, polyphenols, and flavonoids, and antioxidant activities were greater in FRG compared to RG. The contents of total ginsenosides were not significantly different. However, the ginsenoside metabolite content was higher in FRG (14,914.3 lg/mL) compared to RG (5697.9 lg/mL). The tyrosinase inhibitory activity (IC50) of FRG was 27.63 lg/mL, and more potent compared with RG (34.14 lg/mL), (P < .05). The elastase inhibitory activity (IC50) of FRG was 117.07 lg/mL also higher compared with RG (157.90 lg/mL). In a primary skin irritation test, 10% RG and 10% FRG were classified as practically nonirritating materials. In a skin sensitization test, the RG group showed a sensitization rate of 100% and its mean evaluation score of irritation was 1.4, whereas the FRG group showed 20% and 0.2%, respectively. By fermentation of RG, FRG has increased contents of ginsenoside metabolites, such as Rg3, Rg5, Rk1, compound K, Rh1, F2, Rg2, and flavonoids content. Therefore, FRG offers increased antiwrinkle efficacy, whitening efficacy, and reduced toxicological potency compared to RG.KEY WORDS: cosmeceutical fermented red ginseng red ginseng skin safety

show abstract

Section: High-performance Liquid Chromatography Analysis Of Ginsenosidesmentioning

confidence: 99%

Fermenting Red Ginseng Enhances Its Safety and Efficacy as a Novel Skin Care Anti-Aging Ingredient: In Vitro and Animal Study

Lee

Kim

Park

et al. 2012

Journal of Medicinal Food

View full text Add to dashboard Cite

show abstract

“…These preparations, namely, ginseng powder, oral solution and fluid extract, were reported to contain the ginsenosides Rf, Rg1, Rd, Re, Rc, Rb2 and Rb1 in varying concentrations. 25,26,41 A mobile phase composed of MeCN/H2O at a ratio of 82:18 was found to give the best separation of all ginsenosides in the temperature range used in this study. Typical chromatograms of the standard and the three different samples at 5˚C are shown in Fig.…”

Section: Retention Prediction In Different Sample Matricesmentioning

confidence: 88%

“…Samples from ginseng power, fluid extract and oral solution were prepared using a method previously developed by our group. 15,41 This method involves ultrasonic extraction of the ginsenosides from the samples, followed by sample clean-up using solid phase extraction procedures.…”

Section: Standard and Sample Solution Preparationmentioning

confidence: 99%

Retention Prediction Modeling of Ginsenosides on a Polyvinyl Alcohol-bonded Stationary Phase at Subambient Temperatures Using Multiple Linear Regression and Artificial Neural Network

et al. 2008

View full text Add to dashboard Cite

“…Down sizing of sample preparation techniques for liquid-phase separations has been studied along with the effective on-line coupling of the miniaturized sample preparation technique to LC separation methods [56][57][58][59][60][61][62][63][64][65]. As similar to the successful applications of particulate chemically-bonded stationary phases in LC to an extraction medium in solid-phase extraction, several types of fiber-packed capillaries were introduced as the extraction medium in microscale sample preparation techniques Chromatograms for the separation of dodecane, tetradecane and hexadecane on a short polymer-coated fiber-packed capillary column with the rapid temperature programming.…”

Section: Microscale Sample Preparation On Fiber-packed Capillarymentioning

confidence: 99%

Miniaturization for the Development of High Performance Separation Systems

Saito

Ueta

2017

Chromatography

View full text Add to dashboard Cite

Miniaturization is a key technological approach to realize high performance separation systems that have several important features required in modern analytical methods. A smaller scale of the stationary phase synthesis enables a systematic analysis of the retention behavior and also a more efficient development of novel stationary phases in liquid chromatography. Development of microscale sample preparation technique has been regarded as a valuable supporting approach to the development of microscale separation methods. Effective on-line coupling of the microscale sample preparation and separation allows an efficient and rapid analysis without a loss of sensitivity for most of the analytical separations. In this review, development of the miniaturized separation techniques and the applications have been described from the view point of the authors' research group, including the development of novel stationary phase, microscale sample preparation techniques for liquid and gas sample matrices.

show abstract

Solid-Phase Extraction and High-Performance Liquid Chromatography for Simultaneous Determination of Important Bioactive Ginsenosides in Pharmaceutical Preparations

Cited by 21 publications

References 31 publications

Fermenting Red Ginseng Enhances Its Safety and Efficacy as a Novel Skin Care Anti-Aging Ingredient: In Vitro and Animal Study

Fermenting Red Ginseng Enhances Its Safety and Efficacy as a Novel Skin Care Anti-Aging Ingredient: In Vitro and Animal Study

Retention Prediction Modeling of Ginsenosides on a Polyvinyl Alcohol-bonded Stationary Phase at Subambient Temperatures Using Multiple Linear Regression and Artificial Neural Network

Miniaturization for the Development of High Performance Separation Systems

Contact Info

Product

Resources

About