Solid-phase extraction and high-performance liquid chromatography procedures for the analysis of paralytic shellfish toxins

Leão, José Manuel; Gago, Ana; Rodríguez-Vázquez, J.A.; Aguete, E.C; Omil, M.M; Comesana, Mirian Rodriguez

doi:10.1016/s0021-9673(97)01211-9

Cited by 12 publications

(12 citation statements)

References 3 publications

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“…A number of chromatographic methods are available for the analysis of a single cyanotoxin family in cyanobacterial bloom material. These methods are mainly based on high-performance liquid chromatography (HPLC) [10][11][12] and gas chromatography (GC) [13]. Because of the chemical diversity of the cyanobacterial toxins there is no simple and rapid method to determine simultaneously various types of cyanotoxins from a single water bloom sample.…”

Section: Introductionmentioning

confidence: 99%

Analysis of cyanobacterial toxins (anatoxin‐a, cylindrospermopsin, microcystin‐LR) by capillary electrophoresis

Vasas¹,

Gáspár

Páger

et al. 2004

Electrophoresis

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Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) were applied to the simultaneous separation of cyanobacterial toxins (anatoxin-a, microcystin-LR, cylindrospermopsin). The analytical performance data of both methods, optimized for the three toxins, were similar with a precision of migration times smaller than 0.8 RSD% and a detection limit in the range of 1-4 microg/mL, using spectrophotometric detection at 230 nm. Both methods were applied to an analysis of cyanotoxins in water bloom samples and crude cyanobacterial extracts. The results obtained indicate that, for complex matrices, the sequential application of CZE and MEKC is necessary. It is recommended to use both CE techniques for the analysis of the same sample in order to confirm the results by an orthogonal approach.

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Section: Introductionmentioning

confidence: 99%

Analysis of cyanobacterial toxins (anatoxin‐a, cylindrospermopsin, microcystin‐LR) by capillary electrophoresis

Vasas¹,

Gáspár

Páger

et al. 2004

Electrophoresis

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“…Many studies undertaken on molluscan bivalves have shown that microcystins can be accumulated (Sciacchitano & Mopper 1993;Leao et al 1998;Sekiguchi et al 2001;Holmes & Teo 2002;Montojo et al 2006;Naves et al 2006;Etheridge 2010;Vale 2010). However, few of these studies have investigated Pacific oysters.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of freshwater and marine cyanobacteria in the Hokianga region, Northland, New Zealand

Wall

Wood

Orlovich

et al. 2013

New Zealand Journal of Marine and Freshwater Research

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Lake Omapere, Northland, New Zealand, contributes significant inputs of freshwater to Hokianga Harbour, and is subject to toxic cyanobacterial blooms. During summer 2004, ADDA-ELISA analysis indicated microcystins were present in Pacific oysters (Crassostrea gigas) in Hokianga Harbour, leading to farm closures. Once the bloom had abated, continued detection of low microcystin levels suggested toxin production may be from cyanobacteria in the harbour not the lake. In this study, 20 cyanobacterial strains were isolated from Lake Omapere, its outflow and Hokianga Harbour. Morphological and molecular data identified diverse cyanobacterial species (15 Oscillatoriales and five Nostocales), including close relatives of toxin producers. Genes associated with toxin production were not detected in any strains and their cellular extracts were not toxic to Artemia salina larvae. An in vitro feeding trial of Pacific oysters with a microcystin-producing Microcystis aeruginosa strain (CAWBG16) was undertaken. No microcystins were detected in the shellfish using liquid chromatography-mass spectrometry.

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“…To determine the optimum incubation time between sample and Ab-beads for maximum extraction recovery four time points (15,60,120, and 240 mins) were explored (Figure 3). While the 15 minute incubation resulted in a 45 ± 5% recovery of STX, the other three incubation times all resulted in approximately 80% recovery (60 min: 79 ± 4%; 120 min: 81 ± 5%; 240 min: 75 ± 6%).…”

Section: Stx Incubation Time Studymentioning

confidence: 99%

“…Traditionally, solid phase extraction (SPE) has been used extensively in the isolation of STX from various biological matrices in animals [12][13][14][15] and humans including urine, blood, and tissues [16,17] as well as from cyanobacterium cells [18,19]. Different formats of SPE include off-line SPE, where the isolation of analytes and analysis are carried out on different systems, and on-line SPE, where extraction and analysis of the compound of interest is completed by one combined system.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of Saxitoxin in Human Urine Using Immunocapture Extraction and LC–MS

et al. 2018

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Solid-phase extraction and high-performance liquid chromatography procedures for the analysis of paralytic shellfish toxins

Cited by 12 publications

References 3 publications

Analysis of cyanobacterial toxins (anatoxin‐a, cylindrospermopsin, microcystin‐LR) by capillary electrophoresis

Analysis of cyanobacterial toxins (anatoxin‐a, cylindrospermopsin, microcystin‐LR) by capillary electrophoresis

Characterisation of freshwater and marine cyanobacteria in the Hokianga region, Northland, New Zealand

Quantitation of Saxitoxin in Human Urine Using Immunocapture Extraction and LC–MS

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