Solid-phase extraction and gas chromatographic—mass spectrometric determination after hydrolysis of 2-aminofluorene haemoglobin adducts in blood of rats

Scheepers, Paul T.J.; Velders, D.D.; Straetemans, Marcel M. E.; Ouwerkerk, Joyce C.; Vliet, L.A. van; Bos, R. P.

doi:10.1016/0378-4347(93)80110-p

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…It is also similar to liquid-solid extraction (LSE), where the extraction procedure consists in putting the analytes into a vessel with a suitable adsorbent material (solid phase) and shaking for a fixed time (Scheepers et al, 1993;Rosenfeld et al, 1991). The analytes to be extracted are partitioned between a solid and a liquid -rather than between two immiscible liquids as in LLE -and these analytes must have a greater affinity for the solid phase than for the sample matrix (retention or adsorption step).…”

Section: Conventional Column Technology For Solid-phase Extractionmentioning

confidence: 99%

Sample Preparation Techniques for Mass Spectrometry in Proteomics Using Recently Developed Highly Selective Materials

Huck¹,

Bakry²,

Bonn

2005

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Efficient sample preparation in the micro and nano-litre range, prior to analysis of proteins and peptides of biological origin by liquid chromatography (LC), liquid chromatography coupled to mass spectrometry (LC-MS), micro-liquid chromatography (µ-LC) and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, is the key to success for different applications, such as biomarker discovery. Sample preparation in proteomics mainly comprises purification and preconcentration by solid-phase extraction (SPE) using different polymers (polystyrene, poly acrylate, cellulose, etc.) as a stationary phase following several cleaning-up procedures. These polymers can be derivatized with several functional groups, for example, C18, ion-exchanger or immobilized metal affinity chromatography (IMAC) groups. Based on these "traditional" techniques new methods enabling higher selectivity, sensitivity and speed of separation are required. Capillary coatings, for example, with latex particles has been used successfully. Derivatization of these latex particles, for example, with IMAC-functional groups enables a selective purification of phosphorylated or His-tagged proteins. Alternatively, polymeric disks possessing a thickness of approximately 2 mm can be used even in the case of multidimensional separations. In this review, we summarize currently available sample pretreatment techniques, provide an overview on the technical background and discuss the advantages of the individual methods using recently developed selective stationary phases such as silica based materials, polymers, etc.

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Section: Conventional Column Technology For Solid-phase Extractionmentioning

confidence: 99%

Sample Preparation Techniques for Mass Spectrometry in Proteomics Using Recently Developed Highly Selective Materials

Huck¹,

Bakry²,

Bonn

2005

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show abstract

“…The principle of SPE is similar to that of LLE or LSE, involving a partitioning of compounds between two phases. In liquid-solid extraction (LSE), the extraction procedure consists in putting the analytes into a vessel with a suitable adsorbent material (solid phase) and shaking for a controlled time (130). After the distribution equilibrium of the analyte between the two phases has been reached, the phases are separated by filtration or decantation.…”

Section: Basis Of Solid Phase Extraction-spe Theorymentioning

confidence: 99%

Development and validation of high-performance liquid chromatographic methods for the determination of 14-benzodiazepines in bio-fluids

Uddin¹

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AΡΙΣTOTΕΛΕΙΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΘΕΣΣΑΛΟΝΙΚΗΣ Εργαστήριο Αναλυτικής Χηµείας Tµήµα Χηµείας Ανάπτυξη και επικύρωση αναλυτικών µεθόδων για τον προσδιορισµό 1,4-βενζοδιαζεπινών σε βιολογικά υγρά µε την τεχνική της Υγρής Χρωµατογραφίας Υψηλής Πίεσης (HPLC) MOHAMMAD NASIR UDDIN (∆ιδακτορική διατριβή) Ηµεροµηνία της εξέτασης-23/10/2009 Επιτροπή εξέτασης Παπαδογιάννης Ιωάννης, (επιβλέπων) Καθηγητής Αναλυτικής Χηµείας, Tµήµα Χηµείας, Αριστοτέλειο Πανεπιστήµιο Θεσσαλονίκης AΠΘ

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A review of solid phase extraction: Basic principles and new developments

1995

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Solid-phase extraction and gas chromatographic—mass spectrometric determination after hydrolysis of 2-aminofluorene haemoglobin adducts in blood of rats

Cited by 7 publications

References 22 publications

Sample Preparation Techniques for Mass Spectrometry in Proteomics Using Recently Developed Highly Selective Materials

Sample Preparation Techniques for Mass Spectrometry in Proteomics Using Recently Developed Highly Selective Materials

Development and validation of high-performance liquid chromatographic methods for the determination of 14-benzodiazepines in bio-fluids

A review of solid phase extraction: Basic principles and new developments

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