Solid lipid nanoparticles produced through a coacervation method

Abstract

Solid lipid nanoparticles (SLN) of fatty acids (FAs) were prepared with a new, solvent-free technique based on FAs precipitation from their sodium salt micelles in the presence of polymeric non-ionic surfactants: this technique was called 'coacervation'. Myristic, palmitic, stearic, arachidic and behenic acid were employed as lipid matrixes. Spherical shaped nanoparticles with mean diameters ranging from 250 to ∼500 nm were obtained. Different aqueous acidifying solutions were used to precipitate various FAs from their sodium salt micellar solution. Good encapsulation efficiency of Nile Red, a lipophilic model dye, in stearic acid nanoparticles was obtained. The coacervation method seems to be a potentially suitable technique to prepare close to monodisperse nanoparticles for drug delivery purposes.

Solid Lipid Nanoparticles for Potential Doxorubicin Delivery in Glioblastoma Treatment: Preliminary In Vitro Studies

“…A selected acidifying solution (coacervating solution) was then added dropwise. 21 The obtained suspension was then cooled in an ice bath under stirring at 300 rpm. For Doxloaded SLN, Dox ion pair was added to the micellar solution prior to acidification, dissolved in ethanol (dimethyl sulfoxide for SDeS-Dox ion pair).…”

“…[18][19][20] Recently, in our research group, a new solvent-free technique was developed to produce SLN of fatty acids by acidification of a micellar solution of their alkaline salts. 21 As pH is lowered, fatty acids precipitate owing to proton exchange between the acid solution and the alkaline salt: this process can be defined as "coacervation." With this technique, lipophilic drugs can be entrapped within nanoparticles by allowing their dissolution in micellar solution prior to acidification.…”

Preparation of solid lipid nanoparticles through various methods using different precursors

“…In this method interaction b/w micellar solution of fatty acid alkaline solution and acid solution takes place. As proton exchange proceed b/w coacervation solution and soap solution, fatty acid get precipitates 11,12 .…”

Bevacizumab loaded solid lipid nanoparticles prepared by the coacervation technique: preliminaryin vitrostudies

“…Briefly, the sodium salt of the fatty acid is dispersed in water and the suspension is heated to a temperature above its Krafft point. Once a clear solution is obtained, it is acidified with a specific acidifying solution, depending on the fatty acid length and the precipitation of SLN is observed [16]. To complete the precipitation of SLN, the solution is placed in an ice bath.…”

“…Recent studies show that SLN can be taken up by cells without altering their morphological and metabolic characteristics [15]. SLN can be produced with different technologies [12]: an innovative method is the fatty acid coacervation, which is based on precipitation of fatty acids SLN, starting from micellar solutions of their sodium salts by simple acidification [16]. Compared to most techniques described in literature [17], this preparation method allows to obtain SLN without using solvents or expensive machinery.…”