Solid lipid nanoparticles as potential tools for gene therapy: In vivo protein expression after intravenous administration

Pozo-Rodríguez, Ana del; Delgado, Diego; Solinís, Maria Ángelés; Pedraz, José Luís; Echevarrı́a, Enrique; Rodríguez, Juan Manuel; Rodríguez-Gascón, Alicia

doi:10.1016/j.ijpharm.2009.10.020

Cited by 72 publications

(33 citation statements)

References 36 publications

(36 reference statements)

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“…cSLN has been shown to condense DNA into nanometric colloidal particles capable of transfecting mammalian cells in vitro (14 ,15, 18, 20). Very recently, cSLN application for gene delivery has been conveniently investigated showing ability to induce the expression of a foreign protein in vivo (16). This supports the potential of SLNs for gene and vaccine delivery (16,23,24).…”

Section: Percent Of Cells Expressed Gfpmentioning

confidence: 73%

“…Application of cetylpalmitate and Tween 80 for SLN formulation was reported elsewhere (14,15,16). We used a slightly modified method to prepare SLN formulation while different process variables were evaluated in an experimental design matrix consisted of 9 runs (3 2 ) which were obtained by Design-Expert 6.0.6 statistical software.…”

Section: Preparation Of Csln Formulationsmentioning

confidence: 99%

“…As a carrier system, solid lipid nanoparticles have many features that are valuable for nanomedicine. So far, SLNs have been investigated as delivery systems mostly for drugs and rarely for genes (14)(15)(16)(18)(19)(20). In both cases, this delivery system is reported to proceed more efficiently compared to the drug or gene alone.…”

Section: Percent Of Cells Expressed Gfpmentioning

confidence: 99%

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Cationic Solid Lipid Nanoparticles Loaded by Cystein Proteinase Genes as a Novel anti-Leishmaniasis DNA Vaccine Delivery System: Characterization and in vitro Evaluations

et al. 2010

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-Purpose. Leishmaniasis is a major health problem in many tropical and sub-tropical countries and development of a safe and easily-available vaccine has high priority. Although several antigens potentially capable of inducing protective immunity have been studied, in the absence of pharmaceutical industry interest they have remained as fine publications only. Amongst them, Cathepsin L-like cysteine proteinases (CPs) have received considerable attention and type I and II CPs have been used in a heterologous prime-boost vaccination regime for experimental visceral leishmaniasis in dogs. Due to the promising results of the mentioned vaccination regime, we aimed to evaluate cationic solid lipid nanoparticles (cSLNs) for in vitro delivery of cpa, cpb and cpb CTE intended to be used as a cocktail DNA vaccine in our forthcoming studies. Methods: cSLNs were formulated of cetyl palmitate, cholesterol, DOTAP and Tween 80 via melt emulsification method followed by high shear homogenization. Different formulations were prepared by anchoring pDNAs on the surface of cSLNs via charge interaction. The formulations were characterized according to their size and zeta potential as well as pDNA integrity and stability against DNase I treatment. Lipoplexes' cytotoxicity was investigated on COS-7 cells by MTT test. The effect of the DOTAP:pDNA ratio on protection ability and cytotoxicity was also studied. In vitro transfection efficiency was qualified by florescent microscopy and quantified using flow cytometry technique. Results: cSLN-pDNA complexes were formulated with suitable size and zeta potential. Efficiency/cytotoxicity ratio of cSLN-pDNAs formulations was comparable to linear PEI-25kDa-pDNAs polyplexes while exhibiting significantly lower cytotoxicity. Conclusion: Tested formulations were able to deliver immunogenic CP genes efficiently. This data proves the ability of this system as a promising DNA vaccine carrier for leishmaniasis to cover the main drawback of naked pDNA delivery that is rapidly elimination from the circulation.

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Section: Percent Of Cells Expressed Gfpmentioning

confidence: 73%

Section: Preparation Of Csln Formulationsmentioning

confidence: 99%

Section: Percent Of Cells Expressed Gfpmentioning

confidence: 99%

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Cationic Solid Lipid Nanoparticles Loaded by Cystein Proteinase Genes as a Novel anti-Leishmaniasis DNA Vaccine Delivery System: Characterization and in vitro Evaluations

et al. 2010

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“…68 Em outro estudo foi demonstrada a capacidade de transfecção in vivo em camundongos e a expressão gênica pôde ser demonstrada após uma semana da injeção intravítrea. 69 …”

Section: óLeos E Tensoativosunclassified

Biomateriais para formulações de base nanotecnológica visando terapia gênica ocular

et al. 2016

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“…Moreover, it has application in petroleum industry which includes enhanced oil recovery (EOR), bioremediation and waste treatment to remove hazardous materials, pharmaceutical-cosmetics formulas, agriculture, food processing industries, detergents, laundry supplies and paint industries [3][4][5][6]. Recently, biosurfactants have received much attention in nanobiotechnology too [7,8].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Nutrient Requirements and Culture Conditions for the Production of Rhamnolipid from Pseudomonas aeruginosa (MTCC 7815) using Mesua ferrea Seed Oil

2013

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Environmental awareness has led to a serious consideration for biological surfactants and hence nonedible vegetable oils may serve as a substitute carbon source for bio-surfactant production (rhamnolipid) which might be an alternative to complex synthetic surfactants. There are reports of rhamnolipid production from plant based oil giving higher production than that of glucose because of their hydrophobicity and high carbon content. Therefore the contribution of non-edible oil such as Mesua ferrea seed oil could serve as a good carbon source for rhamnolipid production. Moreover the use of rhamnolipid production from non-edible plant based seed oil has not been reported elsewhere. The present work focus on the optimal production of rhamnolipid by considering both micro and macro nutrients and culture conditions using response surface methodology. The study observes that micronutrients play a significant role in rhamnolipid production from Pseudomonas aeruginosa (MTCC 7815). The investigation results with the statistically optimize parameters able to produce a higher rhamnolipid production and this methodology could be used to optimize the nutrients requirements and culture conditions. The present findings would assist in bioremediation of crude oil contaminated ecosystems.

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Solid lipid nanoparticles as potential tools for gene therapy: In vivo protein expression after intravenous administration

Cited by 72 publications

References 36 publications

Cationic Solid Lipid Nanoparticles Loaded by Cystein Proteinase Genes as a Novel anti-Leishmaniasis DNA Vaccine Delivery System: Characterization and in vitro Evaluations

Cationic Solid Lipid Nanoparticles Loaded by Cystein Proteinase Genes as a Novel anti-Leishmaniasis DNA Vaccine Delivery System: Characterization and in vitro Evaluations

Biomateriais para formulações de base nanotecnológica visando terapia gênica ocular

Optimization of Nutrient Requirements and Culture Conditions for the Production of Rhamnolipid from Pseudomonas aeruginosa (MTCC 7815) using Mesua ferrea Seed Oil

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