Solid acids assisted matrix solid‐phase dispersion microextraction of alkaloids by capillary electrophoresis coupled with quadrupole time‐of‐flight mass spectrometry

Abstract

The quantification of three alkaloids is important because quantitative study is a means of assessing the reliability of the experimental method, and three alkaloids of peimine, peiminine, and peimisine are main active ingredients in Chinese Pharmacopoeia 2015. An effective method based on the matrix solid-phase dispersion microextraction was developed for the extraction of alkaloid compounds in Fritillariae Thunbergii Bulbus. Target analytes were analyzed by capillary electrophoresis coupled with quadrupole time-of-flight mass spectrometry. The optimized experimental condition was that 50 mg Fritillariae Thunbergii Bulbus was blended homogeneously with 10 mg citric acid for 5 min. Two hundred microliters of water acidized by 1 mol/L hydrochloric acid (pH = 4.5) was selected to elute tested alkaloids. The results demonstrated that the investigated method had low limits of detection (1.32-1.59 ng/mL), good recoveries (86.63-98.12%), and reproducibility (relative standard deviations of peak areas < 0.87%). The proposed matrix solid-phase dispersion microextraction coupled with capillary electrophoresis combined with quadrupole time-of-flight mass spectrometry was successfully applied for the extraction of alkaloids in plants. K E Y W O R D Scapillary electrophoresis, isosteroidal alkaloids, matrix solid-phase dispersion microextraction, quadrupole time-of-flight mass spectrometry, traditional Chinese medicine

Recent Applications of Capillary Electrophoresis in the Determination of Active Compounds in Medicinal Plants and Pharmaceutical Formulations

“…Wang et al proposed matrix solid-phase dispersion microextraction combined with CE in conjunction with quadrupole time-of-flight mass spectrometry for the quantification of three alkaloids in Fritillariae Thunbergii bulbus . It is noteworthy that in this method the reported LOQ value is in the ng mL −1 level [ 137 ]. All reported CE–MS methods were effectively employed for qualitative and quantitative analysis of bioactive components in plant extract and pharmaceutical preparations (see Table 5 ).…”

Advances and strategies for capillary electrophoresis in the characterization of traditional Chinese medicine: A review of the past decade (2011–2021)

“…Excellent sorbent separation is critical in MSPD because they act as an abrasive substance and a bound sorbent. A practical method based on the MSPD coupled with CE-TOF-MS was developed to extract alkaloid compounds in Fritillariae Thunbergii Bulbus (Wang et al, 2019).…”

“…An effective UAE was also quickly adopted in the sample preparation of TCMs with various physicochemical effects, including ultrasonic cavitation, fragmentation, erosion, and shear forces (Vinatoru et al, 2017). This technique was efficiently utilized to extract active components such as Fufang Danshen (Ji et al, 2016), Schisandra sphenanthera (Yuan et al, 2011), Evodiae Fructus (Liu et al, 2019), Fritillariae Thunbergii Bulbus (Wang et al, 2019), Fructus Psoraleae (Zhang and Chen, 2012), Catha edulis (Roda et al, 2013), and Goji berry (Wang et al, 2018).…”

Simultaneous extraction and determination of alkaloids and organic acids in Uncariae Ramulas Cum Unicis by vortex-assisted matrix solid phase dispersion extraction coupled with UHPLC-MS/MS

“…Recently, an increasing number of modified MSPD extraction methods have been established by researchers, including ultrasound-assisted MSPD (UA-MSPD) (Dos Santos et al, 2019), vortex-assisted MSPD (VA-MSPD) (de Melo Malinowski et al, 2022), and micro salting-out assisted MSPD (µ-SOA-MSPD) (Zhang et al, 2021), which simplify the extraction procedures of traditional MSPD and reduce both the loss of target components and the sample pretreatment time. Currently, these modified MSPD methods have been used for the extraction of active components from various herbs medicines such as terpenoids, flavonoids, and alkaloids (Wang et al, 2019;Chen et al, 2021;Zheng et al, 2021). The modified MSPD method is a good way to avoid the mutual transformation of isomeric alkaloids in Gouteng under heating conditions.…”