Sometimes microorganisms can be utilised for biological conversion of various substrates for the preparation of pharmacologically active compounds. The chemical change of the substrate which is somewhat difficult to accomplish in the chemistry laboratory involving time consuming processes, can easily be achieved by means of enzymatic activities of microorganisms. As these are easy to propagate and study in the laboratory, the knowledge of biological conversion may sometimes be utilised in the commercial production of the pharmaceutically active constituents.Solanum Khasianum C. B. Clarke has been recognised as one of the richest sources of solasodine amongst the indigenous species of the genus so far investigated. The steroidal alkaloid solasodine, a nitrogenous analogue of diosgenin, has immense therapeutic importance which can be utilized as a basic precursor for the synthesis of various corticosteroid drugs and sex hormones including the active constituents of oral contraceptives. Ripe berries of this plant contain various steroidal glycoalkaloids such as solasonine (Choudhuri et al., 1964), solamargine (Seth, 1972, solakhasianin (Maity et al., 1968) and solasurine (Seth, 1972). The aglycone part of all these glycoalkaloids is solasodine, a C2, steroidal alkaloid. Soladodine (AS, 220, 25aspirosolen -38 -01, C27C4302N) can be converted into 36-acyl -A5, 16-pregnadiene-2O-one, from which the corticosteroid compounds and steroidal sex hormones can be synthetized. Though the glycoalkaloids produce solasodine on acid hydrolysis yet an appreciable amount of solasodine is lost due to conversion of the aglycone part to solasodiene, a dehydrated product of solasodine (Briggs et al., 1942; Maity et al., 1968). In this present investigation, attempts have been made to determine the microbiological hydrolysis of the glycoalkaloid of S. khasianum C. B. Clarke into solasodine carried out by a local strain of Aspergillus niger.Air-dried powdered berries (950 gms) were extracted in a 3L Soxhlet with Pharmaceutical Biology Downloaded from informahealthcare.com by Chulalongkorn University on 12/26/14 For personal use only.I54 M. K. LAHA AND P. K. BASUpetroleum ether (B.P. 60"-80") for 48 hrs. The residual meal was again soxhleted with methanol for the same period. The alcoholic fraction was concentrated under reduced pressure. The petrol ether-soluble part was treated with 2% hydrochloric acid and the crude alkaloid was obtained after basification with ammonium hydroxide at 60°C (PH 9.5). The ether-insoluble part was treated with hot water with constant vigorous stirring to remove the traces of petrol ether. The clear liquid was strongly basified with ammonium hydroxide and left overnight at room temperature. TLC showed that the residue containing the glycoalkaloid was devoid of any free solasodine. Crude glycoalkaloid dissolved in saturated solution of sodium chloride was used in malt agar culture medium (pH 4.5) to grow the fungus at 34 * 1°C.After 5 days, a methanolic extract of the culture medium was prepared and concentr...