The vertical and seasonal distribution and diversity of archaeal sequences was investigated in a hypersaline, stratified, monomictic lake, Solar Lake, Sinai, Egypt, during the limnological development of stratification and mixing. Archaeal sequences were studied via phylogenetic analysis of 16S rDNA sequences as well as denaturing gradient gel electrophoresis analysis. The 165 clones studied were grouped into four phylogenetically different clusters. Most of the clones isolated from both the aerobic epilimnion and the sulfide-rich hypolimnion were defined as cluster I, belonging to the Halobacteriaceae family. The three additional clusters were all isolated from the anaerobic hypolimnion. Cluster II is phylogenetically located between the genera Methanobacterium and Methanococcus. Clusters III and IV relate to two previously documented groups of uncultured euryarchaeota, remotely related to the genus Thermoplasma. No crenarchaeota were found in the water column of the Solar Lake. The archaeal community in the Solar Lake under both stratified and mixed conditions was dominated by halobacteria in salinities higher than 10%. During stratification, additional clusters, some of which may possibly relate to uncultured halophilic methanogens, were found in the sulfide-and methane-rich hypolimnion.The Solar Lake is a small monomictic hypersaline lake located on the Sinai coast of the Gulf of Aqaba. The lake is separated from the gulf by a narrow sand bar. Water is supplied via seawater seepage through the sand bar and occasional winter precipitation. The depth of the lake fluctuates between 4 and 6 m. It is regulated by seasonal changes of influx seepage, out-flux of brine back to the gulf, and evaporation. High evaporation rates and arid climatic conditions during the summer months cause the salinity to rise to hypersaline values of up to 20%. Total mixing of the water column then occurs, and the entire water column is completely oxygenated. Temperature and salinity are constant throughout the water column, and sulfide and methane concentrations are very low during holomixis (6). In the fall, the water column becomes stratified due to the salinity gradient between the residual, highly saline water (18 to 20%) and the overlying, newly introduced seawater (5%). The density gradient between the two layers causes development of a pycnocline, which prevents mixing between the epilimnion and the hypolimnion and allows the development of a thermocline with an inverse temperature gradient (16 to 55°C). Oxygen is present throughout the epilimnion of the lake, where oxygen concentrations may reach supersaturation due to the photosynthetic activity of planktonic cyanobacteria (5). The pycnocline prevents atmospheric oxygen from penetrating the hypolimnion, and oxygen is rapidly diminished. High sulfide concentrations of up to 3 mM develop in the hypolimnion due to the activity of sulfate-reducing bacteria (SRB) in the water column (19) and in the surrounding cyanobacterial mats (36). Gradual heliothermal heating of the metalimnio...