Total amount of soluble seed protein along with its protein profile of nine species of Solanum was investigated through SDS-PAGE. S. nigrum and S. macranthum contain maximum and minimum amount of total soluble seed protein per gm of tissue respectively. A dendrogram based on Jaccard's similarity index and also on the basis of presence and absence of peptide bands revealed two major clusters-upper cluster (UC) and lower cluster (LC). Both the clusters are again subdivided in two sub-clusters like UC1, UC2 and LC1, LC2. S. nigrum being evolutionary more closely (91%) related to S. villosum than S. americanum, has been placed in UC1 while S. americanum along with S. sisymbriifolium, S. macranthum and S. torvum are placed within UC2. S. indicum and S. erianthum showed close resemblance and are placed in LC1 while LC2 contains only S. xanthocarpum, which shows least similarity with other studied species of Solanum and thus occupies a distinct place on the dendrogram. Based on these results, the genus Solanum can be divided into two sub genera and the distribution pattern of these species in the two sub genera does not corroborate with the conventional classification. The present study thus provides useful information for the identification of the taxa, their relationship and delimitation of their taxonomic status. So, this omega taxonomical approach may be very much beneficial for future proteomics study.