Sol−Gel Thin-Film Immobilized Soybean Peroxidase Biosensor for the Amperometric Determination of Hydrogen Peroxide in Acid Medium

Wang, Bingquan; Li, Bin; Wang, Zhenxin; Xu, Guobao; Wang, Qun; Dong, Shaojun

doi:10.1021/ac980940q

Cited by 168 publications

(96 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[20][21] Sol-gel electrochemistry that stemmed from the intersection between sol-gel chemistry and electrochemistry has drawn extensive interest over past decades because of its remarkable facilitation of electrochemical studies and applications. [22][23][24][25][26] Increasing interest has been focused on sol-gel-derived carbon ceramic electrodes (CCEs), which are generally prepared by doping carbon or graphite powder, into silicate gel matrix. 27 CCEs have both advantages of carbonbased electrodes and sol-gel electrochemistry.…”

Section: Introductionmentioning

confidence: 99%

Determination of Imidacloprid in Tomato Grown in Greenhouse Based on Copper(II) Phthalocyanine Modified Carbon Ceramic Electrode by Differential Pulse Voltammetry

Majidi

Asadpour‐Zeynali

Bamorowat

et al. 2011

J Chinese Chemical Soc

View full text Add to dashboard Cite

A new sol-gel derived electrocatalytic carbon ceramic electrode was prepared by incorporating copper(II) phthalocyanine (CuPc) in a carbon ceramic network. This electrode was used as a sensitive electrochemical sensor for determination of the insecticide Imidacloprid (1-(6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine) by differential pulse voltammetry (DPV). The resulting modified electrode exhibits a cathodic peak potential shifted positively and an increasing in cathodic peak current in comparison with unmodified electrode. The redox properties of this modified electrode at various pH values and CuPc percentage were investigated. The catalytic current obtained from differential pulse voltammetry is linearly dependent on Imidacloprid concentration over the two linear ranges of 0.67-17 µM and 17-93 µM with correlation coefficient of R 2 = 0.9999 and R 2 = 0.990, respectively. The detection limit for Imidacloprid was found to be 0.28 mM according to lower linear range. Possible interferences from several common pesticides were also evaluated. The inherent stability, high sensitivity, low detection limit and low cost for each preparation are advantages of this sensor. Determination of Imidacloprid in commercial formulation and residual Imidacloprid in tomato grown in greenhouse (protected cultivation) was also conducted. The results obtained from commercial formulation were completely consistent with those obtained through the standard high-performance liquid chromatography (HPLC) method.

show abstract

Section: Introductionmentioning

confidence: 99%

Determination of Imidacloprid in Tomato Grown in Greenhouse Based on Copper(II) Phthalocyanine Modified Carbon Ceramic Electrode by Differential Pulse Voltammetry

Majidi

Asadpour‐Zeynali

Bamorowat

et al. 2011

J Chinese Chemical Soc

View full text Add to dashboard Cite

show abstract

“…Many methods such as titrimetry, UV-vis spectrophotometry and electrochemistry have been developed for this purpose [1][2][3]. Among these methods, the amperometric biosensors based on the direct electron transfer between an electrode and the immobilized enzyme/protein is especially promising because of its simplicity and high sensitivity [4][5][6][7][8][9][10][11][12][13][14][15][16][17]. Horseradish peroxidase (HRP), a prototypical hemeprotein peroxidase (FW ca.…”

Section: Introductionmentioning

confidence: 99%

“…Horseradish peroxidase (HRP), a prototypical hemeprotein peroxidase (FW ca. 44000), is one of the most widely used enzymes for analytical purposes and biosensors [4,5,[7][8][9]11,12,14,17]. This technique has good sensitivity and accuracy, but at the same time, they suffer from some shortcomings.…”

Section: Introductionmentioning

confidence: 99%

A Novel Hydrogen Peroxide Sensor via the Direct Electrochemistry of Horseradish Peroxidase Immobilized on Colloidal Gold Modified Screen-printed Electrode

Liu

2003

Sensors

View full text Add to dashboard Cite

Abstract:The direct electrochemistry of horseradish peroxidase (HRP) immobilized on a colloidal gold modified screen-printed carbon electrode (HRP-Au-SPCE) and its application as a disposable sensor were studied. The immobilized HRP displayed a couple of stable and well-defined redox peaks with a formal potential of -0.338 V (vs. SCE) and a heterogeneous electron transfer rate constant of (0.75±0.04) s -1 in 0.1 M pH 7.0 PBS. It showed a highly thermal stability, fast amperometric response and an electrocatalytic activity to the reduction of hydrogen peroxide (H 2 O 2 ) without the aid of an electron mediator. The biosensor exhibited high sensitivity, good reproducibility, and long-term stability for the determination of H 2 O 2 with a linear range from 0.8 M to 1.0 mM and a detection limit of 0. 4 M at 3σ. The variation coefficients are 2.7 % and 2.3 % for over 10 successive assays at the H 2 O 2 concentrations of 8.0 and 20 M, respectively. The K M app for H 2 O 2 sensor was determined to be 1.3 mM.

show abstract

“…Several analytical methods have been proposed for the determination of H2O2, including spectrophotometry, [5][6][7][8] spectrofluorometry, 9-13 chemiluminescence [14][15][16][17] and electrochemical techniques. [18][19][20][21] Most of these methods are enzymatic methods that are of interest because of their high sensitivity and selectivity.…”

mentioning

confidence: 99%

Application of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Rich Source of Peroxidase in the Spectrofluorometric Determination of Hydrogen Peroxide in Honey Samples

2006

View full text Add to dashboard Cite

Crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared by a simple procedure and its enzymatic activity and total protein concentration were determined. It was found that this crude extract is a rich source of peroxidase (POx) and has high specific activity. Cross-linked polyvinylpyrrolidone was used as a stabilizer in the preparation of the crude extract. The POx activity of kohlrabi crude extract did not vary for at least 2 months when deoxygenated and stored at 4˚C. This extract was applied for the spectrofluorometric determination of hydrogen peroxide using homovanillic acid as a fluorogenic substrate. POx catalyzes the hydrogen peroxide oxidation of homovanillic acid to produce a dimer which shows strong fluorescence at 420 nm with excitation at 312 nm. In the optimum conditions, the calibration graph for hydrogen peroxide was linear up to 190 ng mL -1 , with a detection limit of 4.4 ng mL -1 . The relative standard deviation (RSD) was 1.48% for 50 ng mL -1 hydrogen peroxide. The proposed method was successfully applied to the determination of hydrogen peroxide in honey. The concentration-time profile of H2O2 produced upon dilution of honey was studied and H2O2 contents of some different honeys from various areas of Iran were determined.

show abstract

Sol−Gel Thin-Film Immobilized Soybean Peroxidase Biosensor for the Amperometric Determination of Hydrogen Peroxide in Acid Medium

Cited by 168 publications

References 34 publications

Determination of Imidacloprid in Tomato Grown in Greenhouse Based on Copper(II) Phthalocyanine Modified Carbon Ceramic Electrode by Differential Pulse Voltammetry

Determination of Imidacloprid in Tomato Grown in Greenhouse Based on Copper(II) Phthalocyanine Modified Carbon Ceramic Electrode by Differential Pulse Voltammetry

A Novel Hydrogen Peroxide Sensor via the Direct Electrochemistry of Horseradish Peroxidase Immobilized on Colloidal Gold Modified Screen-printed Electrode

Application of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Rich Source of Peroxidase in the Spectrofluorometric Determination of Hydrogen Peroxide in Honey Samples

Contact Info

Product

Resources

About