Sol-gel (template) synthesis of osteoplastic CaSiO3/HAp powder biocomposite: “In vitro” and “in vivo” biocompatibility assessment

Папынов, Е. К.; Shichalin, O.O.; Апанасевич, В. И.; Mayorov, V. Yu.; I.Yu., Buravlev; Merkulov, E. B.; Kaidalova, T. A.; Modin, Evgeny; Afonin, I.S.; Evdokimov, I.O.; Geltser, B.I.; Zinoviev, S.V.; Stepanyugina, Alexandra; Kotciurbii, E.A.; Bardin, A.A.; Korshunova, Oksana

doi:10.1016/j.powtec.2020.04.040

Cited by 28 publications

(16 citation statements)

References 40 publications

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“…Powder CaSiO 3 /HAp biocomposite was obtained as a structured macropore powder by an original method of sol-gel (template) synthesis proposed earlier by the authors [ 40 ]. The method was based on calcium silicate and Hap hydrogel mixture fabrication with a pore-forming template (siloxane-acrylate latex) additive for macroporous structure organization after thermal treatment, according to the scheme; 150 mL of siloxane-acrylate latex water solution (latex: water ratio 1:30) was added to 50 mL of 1.0 M calcium chloride and 50 mL of 1.0 M sodium metasilicate dropwise under intense stirring.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies conducted by the authors presented original methods for the synthesis of CaSiO 3 /HAp biocomposite as an osteoplastic powder material and ceramic volume matrices with exceptional mechanical characteristics and biocompatible properties [ 40 ]. The results of the study on the disperse system obtained by sol-gel (template) synthesis showed that it was not toxic in the “in vitro” model in the conditions for influencing immune cells.…”

Section: Introductionmentioning

confidence: 99%

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Morphological Characteristics of the Osteoplastic Potential of Synthetic CaSiO3/HAp Powder Biocomposite

Апанасевич

Папынов

Плехова

et al. 2020

JFB

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The study describes the influence of synthetic CaSiO3/HAp powder biocomposite on the process of regeneration in osseous tissue in the alveolar ridges in terms of the morphological characteristics of the osteoplastic potential. The authors investigated the osteoinduction and osteoconduction “in vivo” processes during bone tissue regeneration in the mandible defect area of an experimental animal (rabbit). The possibility of angiogenesis in the graft as an adaptation factor was studied in the process of bone tissue regeneration. The results of the histological study that included the qualitative parameters of bone tissue regeneration, the morphometric parameters (microarchitectonics) of the bone, the parameters of osteosynthesis (thickness of the osteoid plates), and resorption (volume density of the eroded surface) were presented. The results allowed the authors to characterize the possibility of the practical adaptation for synthetic powder biocomposite as an osteoplastic graft for the rehabilitation of osseous defects in dentistry.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological Characteristics of the Osteoplastic Potential of Synthetic CaSiO3/HAp Powder Biocomposite

Апанасевич

Папынов

Плехова

et al. 2020

JFB

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“…Bioactive ceramics and glass-ceramics are a unique group of synthetic materials that react with biological fluids, presenting enhanced capability of biointegration [1][2][3][4][5][6]. Numerous studies, both in vitro and in vivo, have demonstrated the ability of these materials to bond with living tissues, especially bone, through the development of a surface apatite layer [7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties

et al. 2021

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Glass-ceramic nanopowder with a composition of 55SiO2-35CaO-10MgO (mol %) was synthesized by the sol–gel method and was heat treated at three temperatures (T1 = 835 °C, T2 = 1000 °C, T3 = 1100 °C) in order to obtain different materials (C1, C2, C3, respectively) varying in crystal structure. Bioactivity and oxidative stress were evaluated in simulated body fluid (SBF) for various time periods (up to 10 days). The structure of the synthesized materials and their apatite-forming ability were investigated by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy and Energy Dispersive Spectroscopy (SEM/EDS). The antibacterial properties of the synthesized materials were evaluated against three Gram-positive and four Gram-negative bacterial strains and their biocompatibility was verified on a primary cell line of human gingival fibroblasts (HGFs) by the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay. The crystallization of the materials was increased by sintering temperature. Heat treatment did not inhibit the bioactive behavior of the materials as apatite formation started after 3 days in SBF. C2, C3 showed some indications of apatite forming even from the first day. Regarding cell viability, a variety of biological behaviors, concerning both dose and time points, was observed between the positive control and the tested materials by both the MTT assay and oxidative stress analysis. In conclusion, the nanobioceramic materials of this study possess a multitude of attractive physicochemical and biological properties that make them suitable candidates for bone regeneration applications, fillers in nanocomposite scaffolds, or as grafts in bone cavities and periodontal lesions.

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“…Calcium monosilicate, β-wollastonite (CaSiO 3 ), is actively studied at the moment due to its applications in traumatology, orthopedics, dentistry, maxillofacial surgery, and other areas of medicine for the recovery, replacement, and reconstruction of the damaged tissue in a living organism [ 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. Owing to β-wollastonite’s ability to activate the growth of the apatite layer on its surface due to the pronounced osteoconduction and bio-resorption via exchange of Ca 2+ and SiO 3 2− with the bioorganic medium, the material is promising as an artificial bone substitute [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…From an implant manufacturing standpoint, fabrication of the β-wollastonite powder and its composites with the HAp-required characteristics and properties is not difficult. This can be done by sol-gel, hydrothermal, and precipitative synthesis technologies [ 6 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. These approaches are easy to implement and allow to vary the size and shape of the crystallites and to tailor the surface morphology.…”

Section: Introductionmentioning

confidence: 99%

CaSiO3-HAp Structural Bioceramic by Sol-Gel and SPS-RS Techniques: Bacteria Test Assessment

Папынов

Shichalin

Buravlev

et al. 2020

JFB

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The article presents an original way of getting porous and mechanically strong CaSiO3-HAp ceramics, which is highly desirable for bone-ceramic implants in bone restoration surgery. The method combines wet and solid-phase approaches of inorganic synthesis: sol-gel (template) technology to produce the amorphous xonotlite (Ca6Si6O17·2OH) as the raw material, followed by its spark plasma sintering–reactive synthesis (SPS-RS) into ceramics. Formation of both crystalline wollastonite (CaSiO3) and hydroxyapatite (Ca10(PO4)6(OH)2) occurs “in situ” under SPS conditions, which is the main novelty of the method, due to combining the solid-phase transitions of the amorphous xonotlite with the chemical reaction within the powder mixture between CaO and CaHPO4. Formation of pristine HAp and its composite derivative with wollastonite was studied by means of TGA and XRD with the temperatures of the “in situ” interactions also determined. A facile route to tailor a macroporous structure is suggested, with polymer (siloxane-acrylate latex) and carbon (fibers and powder) fillers being used as the pore-forming templates. Microbial tests were carried out to reveal the morphological features of the bacterial film Pseudomonas aeruginosa that formed on the surface of the ceramics, depending on the content of HAp (0, 20, and 50 wt%).

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Sol-gel (template) synthesis of osteoplastic CaSiO3/HAp powder biocomposite: “In vitro” and “in vivo” biocompatibility assessment

Cited by 28 publications

References 40 publications

Morphological Characteristics of the Osteoplastic Potential of Synthetic CaSiO3/HAp Powder Biocomposite

Morphological Characteristics of the Osteoplastic Potential of Synthetic CaSiO3/HAp Powder Biocomposite

Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties

CaSiO3-HAp Structural Bioceramic by Sol-Gel and SPS-RS Techniques: Bacteria Test Assessment

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