Sol–gel process: the inorganic approach in protein imprinting

Gutiérrez-Climente, Raquel; Clavié, Margaux; Dumy, Pascal; Mehdi, Ahmad; Subra, Gilles

doi:10.1039/d0tb02941f

Cited by 13 publications

(10 citation statements)

References 126 publications

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“…In our group, we consider that the grafting of amino acids on a material surface is a way to mimic a protein surface, and may favor interaction with other relevant proteins. This can be applied for the development of protein-imprinted materials by the inorganic approach, where the necessity of new sol–gel precursors has been highlighted [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Amino Acids Functionalized SBA-15 for the Improvement of Protein Adsorption

et al. 2021

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Ordered mesoporous materials and their modification with multiple functional groups are of wide scientific interest for many applications involving interaction with biological systems and biomolecules (e.g., catalysis, separation, sensor design, nano-science or drug delivery). In particular, the immobilization of enzymes onto solid supports is highly attractive for industry and synthetic chemistry, as it allows the development of stable and cheap biocatalysts. In this context, we developed novel silylated amino acid derivatives (Si-AA-NH2) that have been immobilized onto SBA-15 materials in biocompatible conditions avoiding the use of toxic catalyst, solvents or reagents. The resulting amino acid-functionalized materials (SBA-15@AA) were characterized by XRD, TGA, EA, Zeta potential, nitrogen sorption and FT-IR. Differences of the physical properties (e.g., charges) were observed while the structural ones remained unchanged. The adsorption of the enzyme lysozyme (Lyz) onto the resulting functionalized SBA-15@AA materials was evaluated at different pHs. The presence of different functional groups compared with bare SBA-15 showed better adsorption results, for example, 79.6 nmol of Lyz adsorbed per m2 of SBA-15@Tyr compared with the 44.9 nmol/m2 of the bare SBA-15.

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Section: Introductionmentioning

confidence: 99%

Development of Amino Acids Functionalized SBA-15 for the Improvement of Protein Adsorption

et al. 2021

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“…Under optimized conditions, the biosensor provided suitable stability, wide linear range, short response time, and low detection limit, revealing the potential of siloxane-based materials for biomedical and clinical applications. These aspects were also comprehensively discussed in an excellent review by Gutierrez-Climente and co-workers [ 34 ].…”

Section: Molecularly Imprinted Siloxane Layersmentioning

confidence: 99%

“…It has to be pointed out that excellent and complete outlooks of the history of molecular imprinting of silica materials were presented by Cameron and co-workers [ 33 ] as well as by Gutierrez-Climente and co-workers [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

A Fusion of Molecular Imprinting Technology and Siloxane Chemistry: A Way to Advanced Hybrid Nanomaterials

2023

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Molecular imprinting technology is a well-known strategy to synthesize materials with a predetermined specificity. For fifty years, the “classical” approach assumed the creation of “memory sites” in the organic polymer matrix by a template molecule that interacts with the functional monomer prior to the polymerization and template removal. However, the phenomenon of a material’s “memory” provided by the “footprint” of the chemical entity was first observed on silica-based materials nearly a century ago. Through the years, molecular imprinting technology has attracted the attention of many scientists. Different forms of molecularly imprinted materials, even on the nanoscale, were elaborated, predominantly using organic polymers to induce the “memory”. This field has expanded quickly in recent years, providing versatile tools for the separation or detection of numerous chemical compounds or even macromolecules. In this review, we would like to emphasize the role of the molecular imprinting process in the formation of highly specific siloxane-based nanomaterials. The distinct chemistry of siloxanes provides an opportunity for the facile functionalization of the surfaces of nanomaterials, enabling us to introduce additional properties and providing a way for vast applications such as detectors or separators. It also allows for catalyzing chemical reactions providing microreactors to facilitate organic synthesis. Finally, it determines the properties of siloxanes such as biocompatibility, which opens the way to applications in drug delivery and nanomedicine. Thus, a brief outlook on the chemistry of siloxanes prior to the discussion of the current state of the art of siloxane-based imprinted nanomaterials will be provided. Those aspects will be presented in the context of practical applications in various areas of chemistry and medicine. Finally, a brief outlook of future perspectives for the field will be pointed out.

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“…MIPs have found vast applications in the fields of solid phase extraction of natural products [28–33], environmental applications [34–36] and as carriers for drug delivery [37–41]. Also, MIPs were reported to be excellent modifiers in electrochemical sensors designed for different analytes including: pharmaceutical compounds [42–45], metal ions [46, 47] and biological molecules as virus [48, 49], bacteria [50, 51] and proteins [52, 53].…”

Section: Introductionmentioning

confidence: 99%

“…excellent modifiers in electrochemical sensors designed for different analytes including: pharmaceutical compounds [42][43][44][45], metal ions [46,47] and biological molecules as virus [48,49], bacteria [50,51] and proteins [52,53].…”

Section: Introductionmentioning

confidence: 99%

Design and application of molecularly imprinted electrochemical sensor for the new generation antidiabetic drug saxagliptin

et al. 2023

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Saxagliptin (Saxa) belongs to a new generation of antidiabetic pharmaceutical compounds used in combination with healthy diet and exercise to lower blood glucose levels in patients with type 2 diabetes mellitus (T2DM). In this work, we report for the first time a molecularly imprinted polymer (MIP) based electrochemical sensor for the determination of Saxa. Computational calculations were performed, based on which five MIPs were synthesized using Saxa as a template, itaconic acid as a monomer, crosslinked with ethylene glycol dimethacrylate and Di methyl sulfoxide (DMSO) as a porogen with different ratios. Non‐covalent interaction (NCI) analysis has been also conducted, and the obtained isosurface analysis was used for graphical visualization of NCI that could occur in real space as well as for the discrimination between hydrogen bond interaction, Van Der Waals attraction and spatial repulsion. The optimized polymer was incorporated as a modifier for designing an electrochemical sensor comprising MIP and Multiwalled carbon nanotubes (MwCNT) within carbon paste electrode (CPE). The operational variables including incubation time, pH, scan rate, and accumulation time were optimized. The sensor showed linearity over the concentration range (1 × 10−9–1 × 10−15 M) with low limit of detection (LOD) 8 × 10−16 and 2 × 10−16 M on using DPV and EIS, respectively. The sensor was successfully applied for pharmaceutical formulations, urine, and human serum samples with recovery range between 97.45–100.64 %.

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Sol–gel process: the inorganic approach in protein imprinting

Abstract: Proteins play a central role for the signal transmission in living systems since they are able to recognize specific biomolecules acting as cellular receptors, antibodies or enzymes; or being themselves...

Cited by 13 publications

References 126 publications

Development of Amino Acids Functionalized SBA-15 for the Improvement of Protein Adsorption

Development of Amino Acids Functionalized SBA-15 for the Improvement of Protein Adsorption

A Fusion of Molecular Imprinting Technology and Siloxane Chemistry: A Way to Advanced Hybrid Nanomaterials

Design and application of molecularly imprinted electrochemical sensor for the new generation antidiabetic drug saxagliptin

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