Sol–gel immobilized ovarian follicles: collaboration between two different cell types in hormone production and secretion

Catalano, Paolo N.; Bourguignon, Natalia; Álvarez, Gisela Solange; Libertun, Carlos; Díaz, Luis Eduardo; Desimone, Martín Federico; Lux‐Lantos, Victoria

doi:10.1039/c2jm30888f

Cited by 13 publications

(8 citation statements)

References 72 publications

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“…Silicon dioxide, also known as silica is used as a hydrophilic, biocompatible, mechanically strong, pore-size controllable and thermal stable and microbial resistant scaffold [44]. Based on these properties, silica matrices of tetraethoxysilane and tetrakis (2-hydroxyethyl) orthosilicate are applied for encapsulation of rat mature ovarian follicles and steroid hormones are secreted by the follicles [45].…”

Section: Silicamentioning

confidence: 99%

Efficient biomaterials for tissue engineering of female reproductive organs

Tamadon

Park

Kim

et al. 2016

Tissue Eng Regen Med

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Current investigations on the bioengineering of female reproductive tissues have created new hopes for the women suffering from reproductive organ failure including congenital anomaly of the female reproductive tract or serious injuries. There are many surgically restore forms that constitute congenital anomaly, however, to date, there is no treatment except surgical treatment of transplantation for patients who are suffering from anomaly or dysfunction organs like vagina and uterus. Restoring and maintaining the normal function of ovary and uterus require the establishment of biological substitutes that can cover the roles of structural support for cells and passage of secreting molecules. As in the case of constructing other functional organs, reproductive organ manufacturing also needs biological matrices which can provide an appropriate condition for attachment, growth, proliferation and signaling of various kinds of grafted cells. Among the organs, uterus needs special features such as plasticity due to their amazing changes in volume when they are in the state of pregnancy. Although numerous natural and synthetic biomaterials are still at the experimental stage, some biomaterials have already been evaluated their efficacy for the reconstruction of female reproductive tissues. In this review, all the biomaterials cited in recent literature that have ever been used and that have a potential for the tissue engineering of female reproductive organs were reviewed, especially focused on bioengineered ovary and uterus.

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Section: Silicamentioning

confidence: 99%

Efficient biomaterials for tissue engineering of female reproductive organs

Tamadon

Park

Kim

et al. 2016

Tissue Eng Regen Med

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“…From the time Carturan et al [14] first introduced the encapsulation of living microorganisms in sol-gel silica matrices, other researchers reported the extension of this process to other cell types [15,16] such as bacteria, yeast, algae and mammalian cells. These were all successfully immobilized in silica matrices [9,[17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Two schemes for production of biosurfactant from Pseudomonas aeruginosa MR01: Applying residues from soybean oil industry and silica sol–gel immobilized cells

Lotfabad

Ebadipour

Roostaazad

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Rhamnolipids are the most common biosurfactants and P. aeruginosa strains are the most frequently studied microorganisms for the production of rhamnolipids. Eco-friendly advantages and promising applications of rhamnolipids in various industries are the major reasons for pursuing the economic production of these biosurfactants. This study shows that cultivation of P. aeruginosa MR01 in medium contained inexpensive soybean oil refinery wastes which exhibited similar levels and homologues of rhamnolipids. Mass spectrometry indicated that the Rha-C10-C10 and Rha-Rha-C10-C10 constitute the main rhamnolipids in different cultures of MR01 including one of oil carbon source analogues. Moreover, rhamnolipid mixtures extracted from different cultures showed critical micelle concentrations (CMC) in the range of ≃24 to ≃36mg/l with capability to reduce the surface tension of aqueous solution from 72 to ≃27-32mN/m. However, the sol-gel technique using tetraethyl orthosilicate (TEOS) was used as a gentler method in order to entrap the P. aeruginosa MR01 cells in mold silica gels. Immobilized cells can be utilized several times in consecutive fermentation batches as well as in flow fermentation processes. In this way, reusability of the cells may lead to a more economical fermentation process. Approximately 90% of cell viability was retained during the silica sol-gel immobilization and ≃84% of viability of immobilized cells was preserved for 365days of immobilization and storage of the cells in phosphate buffer at 4°C and 25°C. Moreover, mold gels showed good mechanical stability during the seven successive fermentation batches and the entrapped cells were able to efficiently preserve their biosurfactant-producing potential.

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“…17,18 However, only a very small number of studies have been conducted with silica gel encapsulated eukaryotes and almost all of these studies have focused on cell therapy applications. 14,[19][20][21] The earliest report on successful long-term sustained functionality observed in silica encapsulated mammalian cells is with mouse pancreatic islet cells encapsulated in hydrolyzed silica alkoxide precursors. 22 One downside of this approach (not explored in the report) is the decreased viability and function of the cells due to toxicity of ethanol, which is a condensation product of silica gelation.…”

Section: Introductionmentioning

confidence: 99%