The application of two biosurfactants in the removal of copper, zinc, and lead from waste metal dumpsite soil with their efficiencies was compared to soil washing agents as KNO3, Ca(NO3)2 and NaOH. The test soil samples were also spiked separately with different concentrations (50 mg/L, 250 mg/L, 750 mg/L and 1500 mg/L ) of CuSO4, ZnSO4 and Pb(NO3)2. The biosurfactants used were produced by Odoribacter splanchnicus DSM 20712 (WBS1) and an unidentified bacterium clone JX981747 (CMS). Five different treatment set up comprising of different ratios (20:1, 15:1, 10:1, 5:1, and 1:1) with the soil solution constant was respectively used. The heavy metal contents were measured using Atomic Absorption Spectrophotometer and the percentage heavy metal removal efficiency was calculated. The highest concentrations of biosurfactant (20:1) at different spiked concentrations of metallic salts recorded the highest values of copper (95.47%, 95.73%, 91.69%, 78.82%); zinc (97.98%, 98.98%, 97.29%, 96.78%) and lead (97.68%, 93.09%, 88.12%, 84.98%) removal. The percentage metal removed in each treatment increased with increasing concentration of the biosurfactants and washing agents (1:1 to 20:1). The chemical structure of the two biosurfactants analyzed using Gas Chromatography Mass Spectroscopy (GC-MS) depict the major component of biosurfactants produced from Odoribacter splanchnicus DSM 20712 to be Di-n- amyl phthalate while 9, Octadecanoic acid, methyl ester was from unidentified bacterium clone JX981747. The one dimensional paper chromatography showed presence of galactose/glucose, mannose, ribose, rhamnose in the biosurfactants produced from Odoribacter splanchnicus DSM 20712 whereas the unidentified bacterium clone JX981747 produced biosurfactants that contained all sugars except mannose. The test biosurfactants studied showed high levels of copper and lead removal than zinc when compared with the test soil washing agents (KNO3), Ca(NO3)2 and NaOH used in this study. Biosurfactants have thus shown to have the ability to remove metals hence its use requires scaling up for environmental applications.