Poor accessibility and cost of soil testing reduce effectiveness of fertilizer use on small-scale subsistence farms, and inadequate funding promotes adoption of soil tests in developing countries with minimal validation. For example, Mehlich I extraction of phosphorus (P) currently used extensively in Guatemala may not be suitable for Guatemala's broad range of soils. At least four alternatives are available but relatively untested [Bray 1, Mehlich III, Olsen, and pressurized hot water (PHW)]. Pressurized hot water is relatively simple and inexpensive but is not yet tested against other extraction methods under variable P or potassium (K) fertilization levels. To determine whether PHW-extracted nutrients could be used to predict maize yield and nutrient concentration and uptake, soil, plant tissue and grain samples were obtained from a multiple-site field study, and calibration studies were conducted using five rates of P and three rates of K on soils incubated without plants or cropped with maize in greenhouse and field conditions. In the multiple-site field study, maize yield related significantly to PHW-extractable P (r 2 ¼ 0.36) and to leaf P concentration 1815 (r 2 ¼ 0.23), but Mehlich I -extractable P did not. In the two soils used in the greenhouse study, maize yield, vegetative P concentration, and total P uptake by maize were predicted by PHW-extractable P (R 2 ¼ 0.72, 0.75, and 0.90, respectively). In the field experiment, grain yield was not improved by P or K application, but P concentration of maize leaf tissue did relate significantly with PHW-extracted P (R 2 ¼ 0.40). Mehlich I did not. There were no yield responses to K application in any experiment, but relationships defined between extractable K for all five K-extraction procedures and soil-applied K were similarly significant. In comparison, PHW was as good as or better than Olsen whereas Bray 1 and Mehlich III were less consistent. Mehlich I was overall the poorest P extractant. Mehlich I extraction of P should be replaced by one of the four alternatives tested. PHW is the least expensive and, therefore, most viable for use in Guatemala soils.