Soil properties affecting adsorption of plasmid DNA and its transformation efficiency in Escherichia coli

Kunito, Takashi; Ihyo, Yasutaka; Miyahara, Hiromu; Seta, Ryo; Yoshida, Seishi; Kubo, Hisahiko; Nagaoka, Kazunari; Sakai, Masao; Saeki, Kazutoshi

doi:10.1007/s00374-015-1068-2

Cited by 10 publications

(4 citation statements)

References 38 publications

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“…However, it is not clear whether these impacts are directly due to the newly introduced gene or indirectly due to the modification of the rhizosphere chemistry of the GM plants (McGregor and Turner 2000). Many constituents of soils, especially colloidal particles including clay minerals and humic substances, have high affinity to adsorb biological molecules such as DNA and proteins originating from soil microorganisms (Cai et al, 2007;Cai et al, 2008;Kunito et al, 2016). Growing scientific evidence demonstrates that soil can safeguard such biomolecules from biological erosion (Cai et al, 2007;Morrissey et al, 2015) and consequently, the soil colloid-mediated protection mechanism might enable soils to retain concerned specific molecule's genetic and toxic properties for a long time (Cai et al, 2007).…”

Section: Impact Of Gm Crops On Soil Bacteriamentioning

confidence: 99%

Impact of genetically modified crops on rhizosphere microorganisms and processes: A review focusing on Bt cotton

Mandal

Sarkar

Owens

et al. 2020

Applied Soil Ecology

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Section: Impact Of Gm Crops On Soil Bacteriamentioning

confidence: 99%

Impact of genetically modified crops on rhizosphere microorganisms and processes: A review focusing on Bt cotton

Mandal

Sarkar

Owens

et al. 2020

Applied Soil Ecology

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“…In this study, all the released DNA was adsorbed quickly on minerals or composites in 15 min varying with their adsorption affinity for DNA (Figure S4). The minerals coated with high HA content (4.0–20.2 mg/g, Table S1) showed a relatively low adsorption affinity for DNA, facilitating the uptake of DNA by bacteria and subsequent transformation. ,, Meanwhile, except for goethite, phyllosilicates and their organic composites hampered transformation by affecting the activity of signal CSF. The strong adsorption of CSF and inhibition of CSF activity by phyllosilicates may be caused by their high negative charges .…”

Section: Discussionmentioning

confidence: 99%

“…Transformation, which involves the direct uptake of eARGs by competent bacteria, is an important HGT pathway to transmit antibiotic resistance in soils. ,− The high adsorption affinity of clay particles for extracellular DNA (eDNA) has been regarded to be responsible for the low transformation frequency of ARGs in soils. ,, A variety of soil attributes and environmental factors, such as soil pH, ionic strength, and particle size of minerals, , could affect the adsorption of eDNA to soil, thereby altering bacterial transformation. In addition, the aggregation of eDNA induced by soil biochar or Al 3+ released from montmorillonite suppressed the transformation of ARGs to Escherichia coli. − Except for eDNA adsorption, the transformation process relies largely on the interaction of soil minerals with bacteria or signal biomolecules. − For example, during the exposure to kaolinite and montmorillonite, the transformation of Bacillus subtilis was inhibited via downregulating the expression of competence genes .…”

Section: Introductionmentioning

confidence: 99%

Dichotomous Role of Humic Substances in Modulating Transformation of Antibiotic Resistance Genes in Mineral Systems

Huang

Zhu

et al. 2022

Environ. Sci. Technol.

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Widespread antibiotic resistance genes (ARGs) have emerged as a focus of attention for public health. Transformation is essential for ARGs dissemination in soils and associated environments; however, the mechanisms of how soil components contribute to the transformation of ARGs remain elusive. Here we demonstrate that three representative mineral− humic acid (HA) composites exert contrasting influence on the transformation of plasmid-borne ARGs in Bacillus subtilis. Mineral surface-bound HA facilitated transformation in kaolinite and montmorillonite systems, while an inhibitory effect of HA was observed for goethite. The elevated transformation by HA-coated kaolinite was mainly attributed to the enhanced activity of competence-stimulating factor (CSF), while increased transformation by montmorillonite−HA composites was assigned to the weakened adsorption affinity of DNA and enhanced gene expression induced by flagella-driven cell motility. In goethite system, HA played an overriding role in suppressing transformation via alleviation of cell membrane damage. The results obtained offer insights into the divergent mechanisms of humic substances in modulating bacterial transformation by soil minerals. Our findings would help for a better understanding on the fate of ARGs in soil systems and provide potentials for the utilization of soil components, particularly organic matter, to mitigate the spread of ARGs in a range of settings.

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“…Genetic engineering provides one to one basis for improving the stability of plant disease management. GE has beneficial effects for the long term and sustainable production of crops [19] .…”

Section: Genetic Engineering Techniquesmentioning

confidence: 99%

Uses of Different Techniques for the Production of Sustainable Soil and Food

Khan

2020

j. of bot. res.

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Due to increase in the population which is estimated that the human papulation will increased 9.7 billion in 2050. There is also increase the demands of the food productions. That’s why there is need to solve the problems regarding to the production of the food. Major problem of the food production is the shortage of the land due to the low and bad soil structure and quality of the soil. Soil erosion is one of the main issue which is caused due to the used of different chemicals, pesticides and fertilizers which are mainly used for the plant growth and protection but they are the main reasons of the production of the pollution in the soil. There is need of the different new technology for the improvement of the soil structure, quality, its fertility and decontamination of pollution from the soil which are eco-friendly to the environment and have no adverse effect. In this study the role of the different techniques in which genetic engineering, Nano technologies, soil and crop management strategies, integrated pest control management strategies, sustainable remediation techniques, microbial management strategies and the different management stairgates. All these techniques aim to the production of the plants and microbes which are effective against plant disease management. The aim of the use nano agrochemicals and nano sensors for sensing environmental and pathogen conditions against disease management. The aim of the paper to provide the production of the disease resistance plant and the provide balanced nutrients supplements to the soil for the improvement of the soil condition and its fertility. These techniques have economic importance due to the use of the nano agrochemicals which are low cost and have effective and reduce the use of the chemicals substances which have negative effect on the soil fertility.. There are sustainable remediations techniques also discussed which are used for the decontamination of the soil pollution. In this study the main focus on the improve and increase soil fertility which enhance the growth of the plants as well the production of the crop production. The production of the stress and degradation resistance microbes which is important factor for the protection of the soil from degradation or contamination. All the techniques which are used in this paper have no adverse effect they are helpful in the tolerance of the stress conditions.

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Soil properties affecting adsorption of plasmid DNA and its transformation efficiency in Escherichia coli

Cited by 10 publications

References 38 publications

Impact of genetically modified crops on rhizosphere microorganisms and processes: A review focusing on Bt cotton

Impact of genetically modified crops on rhizosphere microorganisms and processes: A review focusing on Bt cotton

Dichotomous Role of Humic Substances in Modulating Transformation of Antibiotic Resistance Genes in Mineral Systems

Uses of Different Techniques for the Production of Sustainable Soil and Food

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