Soil oxidoreductase zymography: Visualizing spatial distributions of peroxidase and phenol oxidase activities at the root-soil interface

Khosrozadeh, Sajedeh; Guber, Andrey; Kravchenko, Alexandra; Ghaderi, Negar; Blagodatskaya, Evgenia

doi:10.1016/j.soilbio.2022.108610

Cited by 16 publications

(8 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As compared with previous studies on the absorption, distribution, and biotransformation of OPEs in plants, ,− ,− we further found that the lateral root can be a major site for the accumulation of OPEs and the transport from the lateral root to the axial root can be a rate-limiting process for the acropetal translocation. Meanwhile, the biotransformation of OPEs in lateral root is also pronounced, which is likely associated with their high enzyme activity per unit area as compared with axial roots . The biotransformation in lateral roots can significantly increase the overall acropetal translocation potential of OPE metabolites, especially for OPdEs.…”

Section: Resultsmentioning

confidence: 76%

“…Meanwhile, the biotransformation of OPEs in lateral root is also pronounced, which is likely associated with their high enzyme activity per unit area as compared with axial roots. 25 The biotransformation in lateral roots can significantly increase the overall acropetal translocation potential of OPE metabolites, especially for OPdEs. OPdEs showed a higher translocation potential as metabolites of OPEs than their direct root exposure.…”

Section: ■ Materials and Methodsmentioning

confidence: 77%

“…Meanwhile, few studies have considered the influence of the tissue differences in root functions on the uptake and acropetal translocation of OPEs. Lateral roots have higher water absorption capacity 24 and more condensed enzyme activity than axial roots, 25 but it remains unclear whether the root tissue difference would affect the fate of OPEs and OPdEs in plants in addition to the knowledge based on a root−shoot dichotomy plant model.…”

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Novel Organophosphate Esters in Hydroponic Lettuces (Lactuca sativa L.): Biotransformation and Acropetal Translocation

Yao

Chen

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

The absorption, translocation, and biotransformation behaviors of organophosphate esters (OPEs) and diesters (OPdEs) in a hydroponic system were investigated. The lateral root was found as the main accumulation and biotransformation place of OPEs and OPdEs in lettuce. The nontarget analysis using high-resolution mass spectrometry revealed five hydroxylated metabolites and five conjugating metabolites in the OPE exposure group, among which methylation, acetylation, and palmitoyl conjugating OPEs were reported as metabolites for the first time. Particularly, methylation on phosphate can be a significant process for plant metabolism, and methyl diphenyl phosphate (MDPP) accounted for the majority of metabolites. The translocation factor values of most identified OPE metabolites are negatively associated with their predicted logarithmic octanol–water partitioning coefficient (log K ow) values (0.75–2.45), indicating that hydrophilicity is a dominant factor in the translocation of OPE metabolites in lettuce. In contrast, palmitoyl conjugation may lead to an enhanced acropetal translocation and those with log K ow values < 0 may have limited translocation potential. Additionally, OPE diesters produced from the biotransformation of OPEs in lettuce showed a higher acropetal translocation potential than those exposed directly. These results further emphasize the necessity to consider biotransformation as an utmost important factor in the accumulation and acropetal translocation potential of OPEs in plants.

show abstract

Section: Resultsmentioning

confidence: 76%

Section: ■ Materials and Methodsmentioning

confidence: 77%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Novel Organophosphate Esters in Hydroponic Lettuces (Lactuca sativa L.): Biotransformation and Acropetal Translocation

Yao

Chen

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Our results are in close agreement to earlier studies by a number of workers, where it has been shown that depending on the chemical structure and concentration, the use of agrochemicals can provide various results (Jastrzębska, 2011;Micuti et al, 2018). PPO plays an important role in respiration, and its activity is high in cropland (Khosrozadeh et al, 2022). Atrazine significantly affected the activity of PPO in the soil at different periods (Yang et al, 2021).…”

Section: Effect Of Tested Fumigants On Ppo Activitymentioning

confidence: 99%

Side-Effect of Soil Fumigation and Soil Drench by some Methyl Bromide Alternatives on three Soil Enzyme Activities

Aly

El-Aswed

Alsahaty

et al. 2023

Alexandria Science Exchange Journal

View full text Add to dashboard Cite

There is an argent need to find safer, environmentally friendlier as well as efficient alternatives. Effect of some fumigants on activities of dehydrogenase (DHase), peroxidase (POD), and polyphenol oxidase (PPO) in soil was carried out. Three types of fumigants were tested: true-fumigants; dimethyl disulfide (DMDS), fumigant generators, phosphine (PH3), 1,4-Dichlorobenzene (1,4-D), and formaldehyde (CH2O), and bio-fumigants an essential oil of garlic, jojoba, citrus, and argan. Mini-atmosphere method was used for fumigant generators and soil drench method was used for true-and bio-fumigants. The results revealed that DMDS at concentration of 2000 and 20 mg/kg inhibited soil enzymes within the first four days, then reach the level of the enzymatic activity of a control soil sample. Almost the same trend of the effect of phosphine was obtained from either aluminum phosphide (AlP) or zinc phosphide (Zn3P2). Phosphine at 5 mg/L caused an increase in the DHase, POD and PPO activities while, at 150 mg/L, it caused inhibition approximately of 25%. Various essential oils proved to be harmless or very low effect for the soil enzymes. For the effect of all fumigants, the maximum enhancement percentage in the DHase was 37.5% while the maximum inhibition was 25%. Also, the maximum induction was 30% while the maximum reduction in POD activity was 42%. In addition, 48% and 40% were the maximum of stimulation and reduction, respectively in the PPO. Therefore, the activities of DHase, POD, and PPO found to be sensitive to fumigants and proved to be a good indicator of the degree of soil contamination with tested fumigants.

show abstract

“…Nevertheless, research is under way to adapt zymography to the micro-scale environment and resolutions (< 100µm), by preparing thin sections from resin impregnated samples and analysing these with epifluorescence microscopy (Ghaderi et al, 2020). Recently, zymography has been adapted to also visualise oxidoreductases (Khosrozadeh et al, 2022), and time lapse imaging has been implemented allowed to better describe enzyme activities by accounting diffusion losses and the kinetics of signa development (Guber et al, 2021). It has, for example, allowed the visualisation of the distribution of phosphatases at the rhizobox scale Razavi et al, 2016;Liu et al, 2017) (Fig.…”

Section: Enzymatic Activitiesmentioning

confidence: 99%

Opportunities and limits in imaging microorganisms and their activities in soil microhabitats

Védère¹,

Laure²,

Nunan³

et al. 2022

Soil Biology and Biochemistry

View full text Add to dashboard Cite

Soil oxidoreductase zymography: Visualizing spatial distributions of peroxidase and phenol oxidase activities at the root-soil interface

Cited by 16 publications

References 52 publications

Identification of Novel Organophosphate Esters in Hydroponic Lettuces (Lactuca sativa L.): Biotransformation and Acropetal Translocation

Identification of Novel Organophosphate Esters in Hydroponic Lettuces (Lactuca sativa L.): Biotransformation and Acropetal Translocation

Side-Effect of Soil Fumigation and Soil Drench by some Methyl Bromide Alternatives on three Soil Enzyme Activities

Opportunities and limits in imaging microorganisms and their activities in soil microhabitats

Contact Info

Product

Resources

About