The organic fraction of soil is known to be composed of the soil biomass, partially decomposed plant and animal residues, and the materials commonly referred to as humic substances. Knowledge of the persistence of these fractions in soil is vital to the understanding of their contribution to soil fertility and soil genesis. Much information concerning the biochemistry of the humus materials also could be obtained through a knowledge of the mean residence times of the various organic fractions.The nature of the organic compounds and of the organo-mineral bonds varies from soil to soil; consequently, the relative stability of the organic matter of different soils should also vary (8, 16). Tyurin investigated the nature of the humus of the main soil types of the U.S.S.R. He found that the chernozemic humic acids were relatively complex with a preponderance of calcium humates, which were said to be very stable. In contrast, the humus of podzolic soils was associated primarily with nonsilicate forms of iron and aluminum and consisted of relatively simple organic structures. These were thought to be relatively mobile and unstable (7, 17).The availability of the carbon-dating technique has made it feasible to determine the mean residence time of soil humic fractions (2, 4, 11). Thus, the general postulations concerning the relative stability of the soil humic components can now be tested. Campbell and Paul (3) discussed the factors which affect the accuracy of the carbon-dating method of analysis when applied to soils. Isotopic fractionation of the carbon isotopes results in a fairly constant correction to each apparent mean residence time (m.r.t.). No large error was observed to result from the increase of nuclear-bomb-produced C14 in the atmosphere; other errors were relatively small. The present study was undertaken to utilize the natural C14 activity of the carbon in the various organic fractions to provide an accurate comparison of the relative stability of a number of soil organic matter fractions.