Soil nitrogen‐hydrolyzing enzyme activity and stoichiometry following a subtropical land use change

Zhang, Qian; Zhang, Dandan; Wu, Jian; Li, Jinsheng; Feng, Jiao; Cheng, Xiaoli

doi:10.1002/ldr.4034

Cited by 17 publications

(15 citation statements)

References 51 publications

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“…In our study, BG, CBH, AP, and (BG + CBH): (NAG + LAP) positively correlated with SWC, similar to the results reported in Larix principisrupprechtii plantations (Qiu et al, 2021). We also observed a negative correlation between pH and enzyme activities and their stoichiometry, consistent with previous findings (Zhang et al, 2021), thus confirming the assumption that soil pH strongly controls enzymatic activities (Cenini et al, 2016). Soil C-, N-, and P-acquiring extracellular enzymes and their stoichiometry varied with elevation in the Helan Mountains.…”

Section: Factors Affecting Enzyme Activities and Stoichiometrysupporting

confidence: 92%

Features and driving factors of microbial metabolic limitation in mountain ecosystems in arid areas: A case study on the Helan Mountains, Northwest China

Pang

Liu²,

Li³

et al. 2022

Front. For. Glob. Change

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Insights into what limits the growth of soil microorganisms in mountain ecosystems increase our understanding of microbial functions and processes. Although the distribution pattern of soil microorganisms in mountain ecosystems has been widely studied, their role in biogeochemical cycles along elevation gradients of mountain ecosystems in arid regions is poorly understood. In this study we analyzed the soil physicochemical properties, soil microbial community structure, extracellular enzymatic activities, ecoenzymatic stoichiometry, microbial metabolism, and their relationships along the 1,300–2,500 m elevational gradient of the Helan Mountains, northwest China. The results showed that the total microbial biomass and its components did not significantly vary with elevation. The GP:GN (gram-positive: gram-negative bacteria) ratios at low elevations were higher than those at the mid and high elevations, indicating enrichment of oligotrophic bacteria at low elevations. The five extracellular enzymes significantly differed with elevation gradient, while the levels of carbon (C)- and nitrogen (N)- acquiring enzymes first increased and then decreased with increasing elevations. Ecoenzymatic stoichiometry indicated that significant limitation of microbial growth by carbon (C) and phosphorus (P) levels occurred at high and medium elevations. Soil physicochemical characteristics, microbial community composition, and ecoenzymatic activities accounted for 43.94 and 22.21% of the microbial C and P restriction, respectively. Our study suggests that mountain ecosystems with high organic C storage possess abundant microbial populations limited by relative C and P. The study also provides important insights linking microbial metabolisms to the environmental gradients in arid mountain ecosystems.

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Section: Factors Affecting Enzyme Activities and Stoichiometrysupporting

confidence: 92%

Features and driving factors of microbial metabolic limitation in mountain ecosystems in arid areas: A case study on the Helan Mountains, Northwest China

Pang

Liu²,

Li³

et al. 2022

Front. For. Glob. Change

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show abstract

“…Moreover, the standard principal axis regression analysis revealed that soil microbes are more highly dependent on (BG + CBH) enzymes than (NAG + LAP) and AP enzymes. Additionally, the vector length was significantly higher at mid and higher altitudes than at lower altitudes, implying a significant C limitation at the mid and higher elevations (Fanin et al, 2016;Zhang et al, 2021). All these results support our first hypothesis.…”

Section: Variations In Ecoenzymatic Stoichiometry and Nutrient Limita...supporting

confidence: 84%

Elevation gradient shapes microbial carbon and phosphorous limitations in the Helan Mountains, Northwest China

Liu

Chen

Pang

et al. 2022

Front. Environ. Sci.

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Soil extracellular ecoenzymatic activities (EEA) are major players in the biogeochemical cycles and are closely related to the metabolic demand and nutrient supply in microbes. However, their effects on biogeochemistry along the elevation gradient on mountain ecosystems in arid regions remain unclear. To address this, we investigated the variations of soil microbial resource limitation and the relative contributing factors along the elevation gradient of the Helan Mountains, northwest China. The results showed that the relative abundance of total microbial, bacterial, fungal, actinomycetes, and N-acquiring enzymatic activities (N-Acetyl-β-D-glucosaminidase and Leucine- α-aminopeptidase, that are NAG and LAP, respectively) in the soil first increased and then decreased with an increase in elevation. This variation pattern could be due to the changes in soil temperature and moisture along the elevation gradient. Soil enzyme stoichiometry and resource allocation further revealed that the microbial metabolism activity in the Helan Mountains was limited by carbon (C) and phosphorus (P). Furthermore, the two limited elements were significantly higher at the mid and high altitudes (2,139–2,438 m) than at low altitudes (1,380–1,650 m). Additionally, redundancy analysis revealed that the soil water content and bulk density played a crucial role in microbial community structures, while the soil pH had the most influence on soil EEA and ecoenzymatic stoichiometry. Our findings revealed the patterns of soil microbial community structure, extracellular enzyme activities, and microbial metabolism at various elevations, which will help in understanding the microbial resource limitation and nutrient cycling in mountain ecosystems in arid regions.

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“…Following this, Jing et al (2019) conducted a vector analysis to determine the spatial distributions of soil microbial metabolic limitations in eight forest ecosystems and found that the relative C limitation of soil microbes was greater in the temperate forests than that in the tropical forests, while soil microbial P versus N limitation decreased with latitude. In addition, based on the vector analysis, some studies showed that soil microbial metabolic limitation sensitively reflected grassland degradation (Dong et al, 2019), vegetation restoration time (Yang et al, 2020), and land use change (Zhang et al, 2021). In comparison with the EES method, vector analysis can synchronously mirror the relative metabolism demands of microbial community, and it was independent of the variations of total enzyme activity (Moorhead et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The pattern of soil microbe metabolic limitation was altered by the increased sheep grazing intensity in two contrasting grasslands: Implications for grassland management in semiarid regions

et al. 2022

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Soil extracellular enzyme stoichiometry (EES) and microbial metabolic limitation deeply reflect soil quality. However, knowledge about the impacts of different grazing intensities on microbial metabolic limitation has not been well-documented.Herein, the influences of four sheep grazing intensities (ungrazed, UG; lightly grazed,LG; moderately grazed, MG; and heavily grazed, HG) on microbial metabolic limitation were investigated in typical steppe (14-year grazing) and desert steppe (17-year grazing). The activities of an extracellular enzyme (EEAs) involved in soil C, N, and P transformation were determined by fluorimetric microplate assay. We found that different grazing intensities significantly affected extracellular enzyme activities and EES. Compared to the treatments of UG, LG, and MG, microbial relative C limitation

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Soil nitrogen‐hydrolyzing enzyme activity and stoichiometry following a subtropical land use change

Cited by 17 publications

References 51 publications

Features and driving factors of microbial metabolic limitation in mountain ecosystems in arid areas: A case study on the Helan Mountains, Northwest China

Features and driving factors of microbial metabolic limitation in mountain ecosystems in arid areas: A case study on the Helan Mountains, Northwest China

Elevation gradient shapes microbial carbon and phosphorous limitations in the Helan Mountains, Northwest China

The pattern of soil microbe metabolic limitation was altered by the increased sheep grazing intensity in two contrasting grasslands: Implications for grassland management in semiarid regions

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