Soil microbiome-mediated salinity tolerance in poplar plantlets is source-dependent

He, Xinghua; Yang, Yefeng; Wei, Huanshen; Youxiang, Zhuang

doi:10.1016/j.chemosphere.2021.129600

Cited by 16 publications

(10 citation statements)

References 60 publications

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“…The concentration and quality of the DNA were evaluated with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, MA, United States). The V3-V4 region of the 16S rRNA gene was amplified using the universal primers (341F: 5′ -CCTACGGGNBGCASCAG-3′ and 806R: 5′ -GACTACNVGGGTATCTAATCC-3′) (He et al, 2021). Every 50 μl PCR reaction contained 2 μl of diluted DNA, 25 μl of 2X Phanta Max Master Mix (Vazyme P515-01, China), 10 pM of forward and reverse primers, and 21 μl of Nuclease-Free Water (PROMEGA).…”

Section: Measurement Of Plant Phenotypic and Physiological Featuresmentioning

confidence: 99%

Drought-induced recruitment of specific root-associated bacteria enhances adaptation of alfalfa to drought stress

Wang

Tang²,

Wang³

et al. 2023

Front. Microbiol.

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Drought is a major abiotic stress that threatens crop production. Soil microbiomes are thought to play a role in enhancing plant adaptation to various stresses. However, it remains unclear whether soil microbiomes play a key role when plants are challenged by drought and whether different varieties are enriched with specific bacteria at the rhizosphere. In this study, we measured changes in growth phenotypes, physiological and biochemical characteristics of drought-tolerant alfalfa (AH) and drought-sensitive (QS) under sterilized and unsterilized soil conditions with adequate watering and with drought stress, and analyzed the rhizosphere bacterial community composition and changes using 16S rRNA high-throughput sequencing. We observed that the unsterilized treatment significantly improved the growth, and physiological and biochemical characteristics of alfalfa seedlings under drought stress compared to the sterilized treatment. Under drought stress, the fresh and dry weight of seedlings increased by 35.24, 29.04, and 11.64%, 2.74% for unsterilized AH and QS, respectively, compared to sterilized treatments. The improvement was greater for AH than for QS. AH and QS recruited different rhizosphere bacteria when challenged by drought. Interestingly, under well-watered conditions, the AH rhizosphere was already rich in drought-tolerant bacterial communities, mainly Proteobacteria and Bacteroidetes, whereas these bacteria started to increase only when QS was subjected to drought. When drought stress was applied, AH was enriched with more drought-tolerant bacteria, mainly Acidobacteria, while the enrichment was weaker in QS rhizosphere. Therefore, the increase in drought tolerance of the drought-tolerant variety AH was greater than that of the drought-sensitive variety QS. Overall, this study confirmed the key role of drought-induced rhizosphere bacteria in improving the adaptation of alfalfa to drought stress, and clarified that this process is significantly related to the variety (genotype). The results of this study provide a basis for improving drought tolerance in alfalfa by regulating the rhizosphere microbiome.

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Section: Measurement Of Plant Phenotypic and Physiological Featuresmentioning

confidence: 99%

Drought-induced recruitment of specific root-associated bacteria enhances adaptation of alfalfa to drought stress

Wang

Tang²,

Wang³

et al. 2023

Front. Microbiol.

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“…4b), indicating that 84K failed to call abundant bene cial bacteria for help to survive salt stress. Taken together, an association between distinct rhizobacterial communities and gene expression patterns occurred in poplar, implying the possibility of a "gene expression-rhizosphere-microbiota-salt tolerance" regulatory process in plant tolerance responses to salinity (Li et al 2021;Yu et al 2021;He et al 2021). However, the detailed molecular mechanisms by which poplar enlists rhizosphere microbiota to enhance salt tolerance require further functional genetic veri cation using transgenic plants.…”

Section: Discussionmentioning

confidence: 87%

Interaction between rhizobacterial community assembly and host responses determines poplar salt tolerance

Liao

Cao²,

Zhang³

et al. 2023

Preprint

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Background and aims Rhizosphere microbes play important roles in plant toleranceto abiotic stresses. Plants of different genetic backgrounds acquire stress resistance by assembling specific rhizosphere microbial communitiesor typical beneficial microbiota. However, the molecular mechanism by whichplants recruit microbiota in response to environmental stresses is unclear. MethodsHere, we investigated transcript regulation in three poplar genotypes, namely, Populus davidiana × P. bolleana Loucne (SXY), P. deltoides × P. euramericana'Nanlin 895' (NL895) and P. alba × P. glandulosa '84K' (84K), and their effect on the rhizosphere microbial community in response to salinization. ResultsThe results showed that SXY exhibited salt tolerance characterized by the best photosynthesis and antioxidant system performance upon salt stress, while salt stress severely damaged the growth and membrane system of 84K. 16S rRNA sequencing revealed the lowest rhizobacterial community diversity associated with SXY compared to 84K and NL895, implying strong enrichment of certain bacterial taxa by the salt tolerant cultivar. Specifically, SXY recruited higher abundances of Hydrogenophaga and Pseudomonas. Furthermore, RNA-seq analysis of roots combined with weighted gene co-expressionnetwork analysis (WGCNA) identified module eigengene (ME)yellow, a module positively related to the SXY-enriched OTUs. KEGG analysis revealedsignificant enrichment of lipid metabolism and amino acid biosynthesis in MEyellow. Twenty-four genes selected from the pathways enriched in MEyellow showed the highest expression levels in SXY. ConclusionsOur results suggest a “gene expression-rhizosphere-microbiota-salt tolerance” regulatory process in the poplar response to salinization and provide new insights into the mechanisms by which plants reshape the soil microbiome to improve salt tolerance.

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“…None of the ME expression patterns was significantly correlated with the abundance profile of OTU12 (Figure 4b), indicating that 84K failed to call abundant beneficial bacteria for help to survive salt stress. Taken together, an association between distinct rhizobacterial communities and gene expression patterns occurred in poplar, implying the possibility of a "gene expression-rhizosphere-microbiota-salt tolerance" regulatory process in plant (He et al, 2021;Li et al, 2021;Yu et al, 2021). However, the detailed molecular mechanisms by which poplar plants enlist rhizosphere microbiota to enhance salt tolerance require further functional genetic verification using transgenic plants.…”

Section: Discussionmentioning

confidence: 99%

Interaction between rhizobacterial community and host root determines poplar salt tolerance

et al. 2023

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Rhizosphere microbes play important roles in plant tolerance to abiotic stresses. Plants of different genetic backgrounds acquire stress resistance by assembling specific rhizosphere microbial communities or typical beneficial microbiota. However, the molecular mechanisms by which plants recruit microbiota during plant acclimation to environmental stresses are unclear. Here, we investigated transcription pattern in three poplar genotypes, namely Populus davidiana × P. bolleana Loucne (SXY), P. deltoides × P. euramericana ‘Nanlin 895’ (NL895) and P. alba × P. glandulosa ‘84K’ (84K), and their effect on the rhizosphere microbial community under salinity. The results showed that SXY exhibited salt tolerance characterized by the best performance of photosynthesis and antioxidant system upon salt stress, while salt stress severely damaged the growth and membrane system of 84K. 16S rRNA sequencing revealed the lowest rhizobacterial community diversity associated with SXY compared to 84K and NL895, implying strong enrichment of certain bacterial taxa by the salt tolerant cultivar. Specifically, SXY recruited higher abundances of Hydrogenophaga and Pseudomonas. Furthermore, RNA‐seq analysis of roots combined with weighted gene co‐expression network analysis (WGCNA) identified module eigengene (ME)yellow, a module positively correlated to the SXY‐enriched OTUs. KEGG analysis revealed significant enrichment of lipid metabolic and amino acid biosynthetic pathways in MEyellow. Twenty‐four genes selected from the pathways enriched in MEyellow showed the highest expression levels in SXY. Our results suggest a “gene expression‐rhizosphere‐microbiota‐salt tolerance” regulatory process in the poplar under salinity and provide new insights into the mechanisms by which plants shape the soil microbiome to improve salt tolerance.

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Soil microbiome-mediated salinity tolerance in poplar plantlets is source-dependent

Cited by 16 publications

References 60 publications

Drought-induced recruitment of specific root-associated bacteria enhances adaptation of alfalfa to drought stress

Drought-induced recruitment of specific root-associated bacteria enhances adaptation of alfalfa to drought stress

Interaction between rhizobacterial community assembly and host responses determines poplar salt tolerance

Interaction between rhizobacterial community and host root determines poplar salt tolerance

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