Soil Microbial Community Analysis using Soil Enzyme Activities in Red Pepper Field Treated Microbial Agents

Kim, Yo-Hwan; Lim, Jeongheui; An, Chang-Hwan; Jung, Byung-Kwon; Kim, Sang-Dal

doi:10.3839/jabc.2011.058

Cited by 11 publications

(5 citation statements)

References 19 publications

(3 reference statements)

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“…In order to further analyze the effects of CSY-P13 and the mixture of FA and PHBA on rhizospheric microorganisms, we determined the activities of soil enzymes, which reflect the composition of soil microbial community ( Kim et al, 2012 ). Firmicutes have an important role on soil C and N cycling ( Ferreira et al, 2015 ), and Proteobacteria and Bacteroidetes are important contributors to N cycling in soil ( van Spanning et al, 2005 ) and induce phosphatase when inorganic phosphate is insufficient ( Sebastian and Ammerman, 2009 ), while Actinobacteria play a critical role in soil C mineralization ( Tardy et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Acinetobacter calcoaceticus CSY-P13 Mitigates Stress of Ferulic and p-Hydroxybenzoic Acids in Cucumber by Affecting Antioxidant Enzyme Activity and Soil Bacterial Community

et al. 2018

Front. Microbiol.

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Ferulic acid (FA) and p-hydroxybenzoic acid (PHBA) are main phenolic compounds accumulated in rhizosphere of continuously cropped cucumber, causing stress in plants. Microbial degradation of a mixture of FA and PHBA is not well understood in soil. We isolated a strain CSY-P13 of Acinetobacter calcoaceticus, inoculated it into soil to protect cucumber from FA and PHBA stress, and explored a mechanism underlying the protection. CSY-P13 effectively degraded a mixture of FA and PHBA in culture solution under conditions of 39.37°C, pH 6.97, and 21.59 g L-1 potassium dihydrogen phosphate, giving rise to 4-vinyl guaiacol, vanillin, vanillic acid, and protocatechuic acid. During FA and PHBA degradation, activities of superoxide dismutase (SOD), catalase, ascorbate peroxidase, and dehydroascorbate reductase in CSY-P13 were induced. Inoculated into cucumber-planted soil containing 220 μg g-1 mixture of FA and PHBA, CSY-P13 degraded FA and PHBA in soil, increased plant height, and decreased malonaldehyde, superoxide radical, and hydrogen peroxide levels in leaves. CSY-P13 also enhanced SOD, guaiacol peroxidase, catalase, glutathione peroxidase, ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase activities; increased ascorbate and glutathione contents; and elevated transcript levels of copper/zinc SOD, manganese SOD, and catalase in leaves under FA and PHBA. Moreover, CSY-P13 increased phosphatase, catalase, urease, and sucrase activities and changed bacterial richness, diversity, and community composition by high throughput sequencing in cucumber-planted soil supplemented with the mixture of FA and PHBA. So CSY-P13 degrades the mixture of FA and PHBA in soil and mitigates stress from the two phenolic compounds in cucumber by activating antioxidant enzymes, changing soil bacterial community, and inducing soil enzymes.

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Section: Discussionmentioning

confidence: 99%

Acinetobacter calcoaceticus CSY-P13 Mitigates Stress of Ferulic and p-Hydroxybenzoic Acids in Cucumber by Affecting Antioxidant Enzyme Activity and Soil Bacterial Community

et al. 2018

Front. Microbiol.

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“…cucumerinum ) and promoted plant growth in vitro, by manipulating the composition of the rhizosphere microbial community, such as increasing bacterial diversity while reducing fungal diversity. Kim et al (2012) found that inoculation with a mixture of PGPB can increase soil microbial community diversity and promote dehydrogenase, urease and phosphatase activities in the soil, while promoting the growth and yield of red pepper. In Chinese pakchoi (Bok choy) crop fields, the Paenibacillus spp.…”

Section: Discussionmentioning

confidence: 99%

“…have become attractive and effective PGPB and BCAs because members of this genus produce antimicrobial compounds that can suppress F. solani under severe environmental conditions (Chen et al, 2016). For example, Kim et al (2012) reported that B. amyloliquefaciens PT14 produces peptides and shows broad‐spectrum antifungal activity against F. solani . More recently, Kurniawan et al (2018) isolated 83 Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

Combining multiple Bacillus spp. with fish protein hydrolysates mitigates root rot (Fusarium solani) and improves cucumber seedlings growth and substrate nutrients

Lin

et al. 2022

Journal of Applied Microbiology

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Aims:The effect of Bacillus strains combined with fish protein hydrolysates (FPHs) on cucumber root rot disease, seedlings growth and substrate nutrients was investigated. Methods and Results:We isolated three strains capable of mitigating cucumber root rot disease, XY-1 and XY-13 strains were identified as B. amyloliquefaciens, and XY-53 strain as B. subtilis. In the absence of bacteria, The 200×dilution (5 ml L −1 ) of FPHs was the optimum concentration for improving cucumber seedlings growth.In vivo antibiosis tests showed that combined bacteria alongside FPHs inhibited the pathogen growth by 85%~90%, higher than individual bacteria. The FPHs combined either with XY-1 and XY-53 strains or with XY-13 and XY-53 strains promoted seedlings growth under infection, whereas FPHs combined with a mixture of XY-1, XY-13 and XY-53 strains showed the highest total phosphorus and organic matter content in substrate. Moreover, FPHs combined with XY-53 strain increased urease activity, while combined either with XY-13 and XY-53 strains or with XY-1, XY-13 and XY-53 strains increased sucrase activity under infection. Conclusions: FPHs combined with B. amyloliquefaciens and B. subtilis had great potential to suppress growth of root rot and promote cucumber seedlings and increase substrate nutrient content.Significance and Impact of the Study: Co-inoculation of B. amyloliquefaciens and B. subtilis with addition of FPHs is a good strategy for maintaining healthy crops.

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“…The phosphatase (acidic and alkaline) enzyme analysis was done by the method of Egamberdieva et al (2010). The urease activity was done by the method of Tabatabai and Bremner (1972) and β-glucosidase activity was done by the method of Kim et al (2012). The microbial biomass was analyzed by the method of Vance et al (1987).…”

Section: Experimental Designmentioning

confidence: 99%

Exploring the benefits of biochar over other organic amendments for reducing of metal toxicity in Withania somnifera

Nigam

Yadav

Khare

et al. 2019

Biochar

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Biochar is considered as a good metal sequester and ameliorates the metal toxicity and uptake in plants. However, its effectiveness over other organic amendments is not well discussed. The aim of this study is to compare the biochar amendments alleviating the levels of Pb and Cd in Withania somnifera L. Dunal with other organic manure. Farmyard manure, vermicompost, and biochar were applied in the field spiked with highly available Pb and Cd. Metal bioavailability and accumulation, plant growth and plant physiology, antioxidant enzymes and metabolite content of W. somnifera along with soil properties were evaluated in all treatments. Study indicates that the application of organic manures significantly alleviated the metal levels (33-72%) in the plant in comparison with control. The herbage yield was significantly higher (33-69%) under different organic manures as compared to the metal treatments. Secondary metabolite contents and antioxidant enzymes were higher in metal treatments in comparison with control. Organic manures were not only able to restrain the Pb and Cd in soil but also enhanced the soil microbial activities. Results indicate that biochar amendments were more promising than farmyard manure and vermicompost due to the presence of more stable carbon in biochar and more alleviation in metal uptake. In addition, net profit in the cultivation of W. somnifera was higher for biochar amendments (50% higher) compared to control. The study recommended that biochar could be a better option for commercial and safer production of W. somnifera.

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Soil Microbial Community Analysis using Soil Enzyme Activities in Red Pepper Field Treated Microbial Agents

Cited by 11 publications

References 19 publications

Acinetobacter calcoaceticus CSY-P13 Mitigates Stress of Ferulic and p-Hydroxybenzoic Acids in Cucumber by Affecting Antioxidant Enzyme Activity and Soil Bacterial Community

Acinetobacter calcoaceticus CSY-P13 Mitigates Stress of Ferulic and p-Hydroxybenzoic Acids in Cucumber by Affecting Antioxidant Enzyme Activity and Soil Bacterial Community

Combining multiple Bacillus spp. with fish protein hydrolysates mitigates root rot (Fusarium solani) and improves cucumber seedlings growth and substrate nutrients

Exploring the benefits of biochar over other organic amendments for reducing of metal toxicity in Withania somnifera

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